Metabolite analysis and nucleoside determination using reproducible UHPLC-Q-ToF-IMS in <i>Ophiocordyceps sinensis</i>

Joshi, Robin; Sharma, Aakriti; Thakur, Krishana; Kumar, Dinesh; Nadda, Gireesh

doi:10.1080/10826076.2018.1541804

Cited by 12 publications

(13 citation statements)

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“…The nucleosides/nucleobases determination was performed according to the earlier validated method [57] . Briefly, the concentration of 1 mg/mL of each stock solution for the standards were prepared in HPLC grade methanol.…”

Section: Methodsmentioning

confidence: 99%

“…Metabolites profiling was performed using gradient elution and mass parameters described by Joshi et al. 2018 [57] . Briefly, a high‐resolution 6560 Ion Mobility Q‐TOF/MS (Agilent, Santa Clara, USA) tandem with Agilent 1290 Infinity II UHPLC system and Mass Hunter software (Agilent Technologies, USA) was used to acquire and process data.…”

Section: Methodsmentioning

confidence: 99%

“…The nucleosides/nucleobases determination was performed according to the earlier validated method. [57] Briefly, the concentration of 1 mg/mL of each stock solution for the standards were prepared in HPLC grade methanol. The identification and quantification were performed on the Eclipse Plus C18 RRHD column (2.1 × 150 mm, 1.8 mm, Agilent Technologies), operated by Agilent 1290 Infinity II UHPLC system (Agilent, Santa Clara, USA).…”

Section: Determination Of Nucleosides/nucleobasesmentioning

confidence: 99%

“…2018. [57] Briefly, a high-resolution 6560 Ion Mobility Q-TOF/MS (Agilent, Santa Clara, USA) tandem with Agilent 1290 Infinity II UHPLC system and Mass Hunter software (Agilent Technologies, USA) was used to acquire and process data. The drying gas temperature: 350 °C, gas flow: 12.0 L/min, nebulizing gas pressure: 30 psi, capillary voltage: 3500 V, and fragmentor voltage: 400 V. The scanning of the mass spectrometer was ranging from 100 -1700 m/z on full scan mode at a speed of 1.50 spectra/s.…”

Section: Metabolites Profiling Using Uhplc-qtof-msmentioning

confidence: 99%

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A Comprehensive Search of the Primary and Secondary Metabolites and Radical Scavenging Potential of Trillium govanianum Wall. ex D. Don

Kumar

Joshi

Sharma

et al. 2021

Chemistry & Biodiversity

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Trillium govanianum rhizomes are traditionally consumed as a raw powder and decoction for the treatment of health complications. Hence, the present study aimed to investigate whether aqueous and alcoholic extracts of T. govanianum rhizomes under hot and cold extraction conditions have similar or dissimilar chemical, nutrient, and antioxidant profiles. The total phenolics, flavonoids, carbohydrates, proteins, fats, and energy values were estimated in all the conditionally prepared samples. The total phenolics (21.23±1.4 mg GAE/g extract), flavonoids (70.57±3.24 mg RE/g extract) were found higher in hot ethanolic extract (TGHEt), while cold water extract (TGGC) showed higher nutrients including amino acids (10.545±0.219 mg/g) and nucleosides (1.803±0.018 mg/g). The nutrient energy value (2.60 and 2.49 Kcal/g extract) was higher in cold and hot ethanolic extracts. Further, TGHEt scavenged the DPPH. (IC50; 870±22 μg/mL) and ABTS.+ (IC50; 80±1.49 μg/mL) effectively and proved its highest antioxidant activity compared to other samples. In LC/MS/MS‐based metabolite profiling, twenty‐six metabolites (fatty acids, steroidal saponins, triterpene saponins, ecdysteroid hormones) were confirmed with mass fragmentation and literature, while one hundred nine metabolites were identified using the METLIN database. The principal component analysis showed clustering of hot condition extracts while cold extracts were differentially located in quadrants. The heatmaps exhibited the associations and differences between metabolite composition, solvents, and extraction conditions. The identified metabolites speculatively predicted the biosynthesis pathway of T. govanianum. Findings also illustrated that T. govanianum is a source of bioactive nutritional components and saponins. The current metabolite profiling of T. govanianum will help in its agricultural and biotechnological interventions for higher quality produce.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Determination Of Nucleosides/nucleobasesmentioning

confidence: 99%

Section: Metabolites Profiling Using Uhplc-qtof-msmentioning

confidence: 99%

See 2 more Smart Citations

A Comprehensive Search of the Primary and Secondary Metabolites and Radical Scavenging Potential of Trillium govanianum Wall. ex D. Don

Kumar

Joshi

Sharma

et al. 2021

Chemistry & Biodiversity

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“…To date, scientists have developed a series of techniques to separate plant metabolites, such as HPLC [8,9], CE [10], HPLC–MS [11, 12], and HPLC–ion mobility spectrometry (IMS) [13]. Although HPLC and CE are two common methods for analyzing plant metabolites, the interferes and coeluted compounds based on the consistency in retention time may lead to false‐positive results.…”

Section: Introductionmentioning

confidence: 99%

A rapid liquid chromatography‐electrospray ionization‐ion mobility spectrometry method for monitoring nine representative metabolites in the seedlings of cucumber and wheat

Yuan

et al. 2020

J of Separation Science

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A fast and convenient high‐performance liquid chromatography‐electrospray ionization‐ion mobility spectrometry method was developed to determine nine representative metabolites in the seedlings of cucumber and wheat. The analytical conditions were obtained by optimizing the parameters of high‐performance liquid chromatography and ion mobility spectrometry. Briefly, acetonitrile–0.1% formic acid solution was selected as the mobile phase for gradient elution at a flow velocity of 0.4 mL/min. Under negative electrospray ionization mode, spray voltage of ion mobility spectrometry was 4.5 kV, and drift tube temperature was set at 90°C. The metabolites from seedling leaves were extracted using 80% acetonitrile as the solvent at 4°C for 12 h. Results showed that under soilless culture conditions, the contents of maltose, citric acid, and p‐hydroxybenzoic acid in the seedlings of cucumber and wheat were reduced by low concentration of itaconic acid, succinic acid, and citric acid. Importantly, this analytical approach demonstrated high sensitivity, good linear response, and high selectivity. The lowest limit of detection was 0.004 μg for p‐hydroxybenzoic acid. Overall, this high‐performance liquid chromatography‐electrospray ionization‐ion mobility spectrometry method is sensitive and efficient for rapid separation and identification of plant metabolites.

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Identification and quantification of eight alkaloids in Aconitum heterophyllum using UHPLC‐DAD‐QTOF‐IMS: A valuable tool for quality control

Punia

Joshi

Kumar

2022

Phytochemical Analysis

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Introduction: Aconitum spp. are prime medicinal plants rich in alkaloids and have been used as the main constituents of traditional medicine in India and China. The whole plant can be toxic and creates pathophysiological conditions inside the human body. Therefore, simultaneous quantification of alkaloids within plant parts and herbal medicines associated with this genus is essential for quality control.Objective: We aimed to develop and validate methods using ultra-high-performance liquid chromatography-diode array detector-quadrupole time-of-flight ion mobility mass spectrometry (UHPLC-DAD-QTOF-IMS) and to develop an analytical strategy for the identification and quantification of alkaloid compounds (aconitine, hypaconitine, mesaconitine, aconine, benzoylmesaconitine, benzoylaconine, bulleyaconitine A, and deoxyaconitine) from Aconitum heterophyllum. Methodology:We developed a simultaneous identification and quantification method for eight alkaloids using UHPLC-DAD-QTOF-IMS. The method was validated as per International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines and also in IMS mode. Results:The developed method has good linearity (r 2 = 0.997-0.999), LOD (0.63-8.31 μg/mL), LOQ (0.63-2.80 μg/mL), recovery (86.01-104.33%), reproducibility, intra-and inter-day variability (<3.25%), and stability. Significant qualitative and quantitative variations were found among different plant parts (flower, leaf, stem, root, and tuber) and five market products of A. heterophyllum. Furthermore, a total of 21 metabolites were also profiled based on the fragmentation pattern of MS 2 using the validated method.Conclusion: An appropriate mobile phase using acetonitrile and water in a gradient elution gave a satisfactory chromatographic separation of eight Aconitum alkaloids with their adjacent peaks. Therefore, this method could provide a scientific and technical platform for quality control assurance.

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Metabolite analysis and nucleoside determination using reproducible UHPLC-Q-ToF-IMS in Ophiocordyceps sinensis

Cited by 12 publications

References 58 publications

A Comprehensive Search of the Primary and Secondary Metabolites and Radical Scavenging Potential of Trillium govanianum Wall. ex D. Don

A Comprehensive Search of the Primary and Secondary Metabolites and Radical Scavenging Potential of Trillium govanianum Wall. ex D. Don

A rapid liquid chromatography‐electrospray ionization‐ion mobility spectrometry method for monitoring nine representative metabolites in the seedlings of cucumber and wheat

Identification and quantification of eight alkaloids in Aconitum heterophyllum using UHPLC‐DAD‐QTOF‐IMS: A valuable tool for quality control

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