Metabolismo oxidativo e perfil bioquímico de ovelhas santa inês no período periparto: efeito da suplementação parenteral com vitamina E

Abstract

ResumoForam utilizadas 14 ovelhas Santa Inês, hígidas, no último mês de gestação que receberam duas aplicações de solução fisiológica (grupo controle-GC), ou 200 mg de acetato de α-tocoferol (vitamina E) (grupo tratado-GT), com intervalo de 14 dias, aos 21 e de 1 a 7 dias antes do parto, respectivamente para a primeira e segunda doses. As amostras de sangue foram coletadas previamente à primeira aplicação (M0), duas semanas após a primeira aplicação (M1), no parto (M2), uma semana (M3), duas semanas (M4) e quatro semanas após o parto (M5). Foram analisadas as concentrações de proteína total, albumina, globulina, ureia, creatinina, ácido úrico, colesterol, triglicérides, glicose, beta hidroxibutirato (BHB), ácidos graxos não esterificados (AGNEs); e as atividades séricas de creatinofosfoquinase (CK), aspartatoaminotransferase (AST) e gamaglutamiltransferase (GGT). Do metabolismo oxidativo foram determinadas as atividades da superóxido dismutase (SOD), glutationa peroxidase (GSH-Px), glutationa reduzida (GSH) e habilidade de redução férrica plasmática (HRFP). Não foram observadas diferenças entre GT e GC nas concentrações de proteína total, globulinas, CK, ácido úrico, glicose, triglicérides, BHB, AGNES, SOD, GSH-Px e GSH. Porém foram observadas maiores concentrações de albumina em M0 (P=0,039); ureia em M1 (P=0,018), M2 (P=0,005) e M3 (P=0,040); creatinina em M2 (P=0,030) e M3 (P=0,047); GGT em M1 (P= 0,01) e M2 (P=0,024), colesterol em M2 (P=0,041) e HRFP em M3 (P= 0,022) para GT em relação ao GC. A AST foi maior para o GC em relação ao GT em M2 (P=0,030). A aplicação de vitamina E (200 UI, via IM) aumentou a HRFP no pós-parto. Palavras-chave: Antioxidante, perfil metabólico, ruminantes AbstractFourteen healthy Santa Inês sheep, in the last month of pregnancy, were used. They were divided into two groups who received or two injections of saline solution (control group -CG), or 200 mg of α-tocopherol acetate (vitamin E) (treated group -TG) with range of 14 days at 21 and 1 to 7 days before delivery, respectively, to the first and second doses. Blood samples were collected prior to the first application (T0) two weeks after the first application (M1), at delivery (M2), one week (M3), two

Serum biochemical reference ranges for pregnant sheep

“…In an assay with Sakiz-Awassi crossbreed sheep in early and late pregnancy, the serum creatinine levels were 0.84 and 0.65 mg/dL, respectively (Yokus et al 2006). The values were higher in Santa Inês hair sheep at late gestation (Nascimento et al 2015) reaching 0.95 mg/dL, but still within limits determined by our study. However, for sheep diagnosed with pregnancy toxemia, Santos et al (2011) reported a mean serum creatinine concentration of 2.01 mg/dL.…”

“…Creatinine excretion is primarily modified by the urinary flow velocity, and levels above the physiological values have been observed as the glomerular filtration rate decreases (Ognik et al 2015). High levels of creatinine may indicate renal failure (Nascimento et al 2015). For serum creatinine, the values found in the literature are lower than that reported by Kaneko, Harvey and Bruss (2008).…”

“…Despite the physiological ranges determined in this study for serum aspartate aminotransferase in pregnant sheep were similar to those of Kaneko, Harvey and Bruss (2008), some studies reported values more like the interval found in this work. Nascimento et al (2015) observed a serum aspartate aminotransferase level of 53.7 U/L in Santa Inês sheep three weeks before lambing, and Yokus et al (2006) reported a mean of 46.23 U/L in Sakiz-Awassi crossbreed sheep during early pregnancy. In Merino landschaf pregnant ewes on the 20th day before lambing, the serum AST concentration was 104.87 U/L), whereas it was 97.20 U/L in Tsigai ewes (Antunović et al 2011).…”

Pre- and post-partum seric biochemical variables of Lacaune ewes naturally infected by gastrointestinal parasites

“…In a similar study conducted by Nascimento et al (2014) in sheep, oxidative metabolism was evaluated by the activities of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), as well as reduced glutathione concentration (GSH) and ferric reducing ability of plasma (FRAP), where FRAP level was the only variable influenced, and this reduced because of the lower antioxidant capacity, especially at the time when there were greater energy demand (partum/lactation) and, therefore, increased metabolic activity of these animals. The degree of parasitism by gastrointestinal helminths did not affect the status oxidant/antioxidant, which may be a consequence since all animals from both groups had higher EPG counts.…”

“…The transition period in dairy sheep can be a critical time due to many physiological changes that an animal goes through from pregnancy, labor, lambing, and milking with significant changes on seric biochemical variables (Nascimento et al 2014). Furthermore, the degree of infection by gastrointestinal parasites affects these changes, causing adverse effects for the animal.…”