Metabolism profiling of nevadensin in vitro and in vivo by UHPLC-Q-TOF-MS/MS

Liang, Caijuan; Zhang, Xia; Diao, Xingxing; Liao, Man; Sun, Yupeng; Zhang, Lantong

doi:10.1016/j.jchromb.2018.03.032

Cited by 29 publications

(18 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Peak 12 was identified as rutin (C 27 H 29 O 16 ) and peak 13 has been characterized as the methylated quercetin derivative isorhamnetin (C 16 H 11 O 7 ), while peak 10 showing a parent ion at m / z : 447.09088 and a daughter ion at m / z : 285.03894 (kaempferol) as a kaempferol hexoside, possibly kaempferol 3- O -glucoside or kaempferol 3- O -galactoside, the first one has been regarded as antiaging compound [40] and affects the endothelial function of Ginkgo biloba extract [41] and the second prevented carbon tetrachloride-induced liver injury in mice, being regarded as a liver protective compound [42]. Peak 24 was tentatively identified as the demethylated rhamnacin (3,7-dimethyl quercetin, C 17 H 13 O 7 ), and peak 29 as nevadesin (5,7-dihydroxy-6,8,4′-trimethoxyflavone) which has a variety of pharmacological effects such as anti-mycobacterium tuberculosis, antitussive, anti-inflammatory, antihypertensive and free radical-scavenging activities effects [43,44,45]. Likewise, peaks 22 and 23 were identified as dimethyl myricetin derivatives one of them probably syringetin [46] (demethylated molecules at 330.03610, (demethylated molecule) 315.01309 (didemethylated molecule).…”

Section: Resultsmentioning

confidence: 99%

Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

et al. 2019

View full text Add to dashboard Cite

The decoction of the local plant Baccharis grisebachii is used as a digestive, gastroprotective, external cicatrizing agent and antiseptic in Argentine. A lyophilized decoction (BLD) from the aerial parts of this plant was evaluated regarding its anti-ulcer, antioxidant and cytotoxic activities and the bioactivities were supported by UHPLC-MS metabolome fingerprinting which revealed the presence of several small bioactive compounds. The antioxidant properties were evaluated by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The BLD showed a moderate free radical scavenging activity in the DPPH (EC50 = 106 µg/mL) and lipid peroxidation in erythrocytes assays (67%, at 250 µg/mL). However, the BLD had the highest gastroprotective effect at a dose of 750 mg/kg with a ninety-three percent inhibition of damage through a mechanism that involve NO and prostaglandins using the ethanol-induced gastric damage in a standard rat model. On the other hand, BLD does not induce cytotoxic changes on human tumor and no-tumor cell lines at the concentrations assayed. Regarding the metabolomic analysis, thirty-one compounds were detected and 30 identified based on UHPLC-OT-MS including twelve flavonoids, eleven cinnamic acid derivatives, one coumarin, one stilbene and two other different phenolic compounds. The results support that the medicinal decoction of Baccharis grisebachii is a valuable natural product with gastroprotective effects and with potential to improve human health that opens a pathway for the development of important phytomedicine products.

show abstract

Section: Resultsmentioning

confidence: 99%

Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The product ion at m / z 285.0398 was created by dropping CO from the ion at m / z 313.0348. Last but not the least, the conspicuous product ion at m / z 147.0461 was formed because of the Retro-Diels-Alder (RDA) reaction in ring C of the flavonoid, which gained the ion at m / z 132.0214 by loss of CH 3 [28]. The MS/MS spectrum and the fragmentation pathways of eupatorin are shown in Figure 2.…”

Section: Resultsmentioning

confidence: 99%

Identification of Metabolites of Eupatorin in Vivo and in Vitro Based on UHPLC-Q-TOF-MS/MS

Chen

Xue

et al. 2019

Molecules

Self Cite

View full text Add to dashboard Cite

Eupatorin is the major bioactive component of Java tea (Orthosiphon stamineus), exhibiting strong anticancer and anti-inflammatory activities. However, no research on the metabolism of eupatorin has been reported to date. In the present study, ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) combined with an efficient online data acquisition and a multiple data processing method were developed for metabolite identification in vivo (rat plasma, bile, urine and feces) and in vitro (rat liver microsomes and intestinal flora). A total of 51 metabolites in vivo, 60 metabolites in vitro were structurally characterized. The loss of CH2, CH2O, O, CO, oxidation, methylation, glucuronidation, sulfate conjugation, N-acetylation, hydrogenation, ketone formation, glycine conjugation, glutamine conjugation and glucose conjugation were the main metabolic pathways of eupatorin. This was the first identification of metabolites of eupatorin in vivo and in vitro and it will provide reference and valuable evidence for further development of new pharmaceuticals and pharmacological mechanisms.

show abstract

“…All animal experiments were performed according to the guidelines of the experimental animal management committee of Hebei Medical University (Shijiazhuang, China). 41,42 This study was also performed in strict accordance with the NIH guidelines for the care and use of laboratory animals (NIH publication no. 85-23 Rev.…”

Section: Methods Validation Of the Pharmacokinetics Study Of Buddleja Lindleyana Fort Extractmentioning

confidence: 99%

The development and validation of a sensitive HPLC-MS/MS method for the quantitative and pharmacokinetic study of the seven components of Buddleja lindleyana Fort.

Zhang

et al. 2021

RSC Adv.

Self Cite

View full text Add to dashboard Cite

show abstract

Metabolism profiling of nevadensin in vitro and in vivo by UHPLC-Q-TOF-MS/MS

Cited by 29 publications

References 17 publications

Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

Identification of Metabolites of Eupatorin in Vivo and in Vitro Based on UHPLC-Q-TOF-MS/MS

The development and validation of a sensitive HPLC-MS/MS method for the quantitative and pharmacokinetic study of the seven components of Buddleja lindleyana Fort.

Contact Info

Product

Resources

About