Metabolism of rubella virus-infected BHK21 cells Enhanced glycolysis and late cellular inhibition

Vaheri, Antti; Cristofalo, Vincent J.

doi:10.1007/bf01241741

Cited by 22 publications

(14 citation statements)

References 22 publications

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“…There were further suggestions of mitochondrial involvement in RV infection when the same group reported a decrease in the level of ATP in RV-infected BHK-21 cells within the first hour of infection coincident with the period of viral adsorption and penetration (6). In addition, there appeared to be an increase in respiration, glycolysis, and alanine synthesis during the same period (8,145).…”

Section: Mitochondrial Changesmentioning

confidence: 95%

“…In addition, organs of congenitally infected infants are smaller than those of uninfected infants and contain fewer cells (159). A depressed mitotic activity has been shown in congenitally infected embryonic primary cell cultures (128), and a slowing of cell division has also been shown for in vitro RV infections of human fetal cells (117) and BHK-21 cells (145). Furthermore, Plotkin and Vaheri (118) claimed to have extracted a protein from RV-infected human fetal cells that was capable of inhibiting mitosis in uninfected cells.…”

Section: Teratogenicity: Concluding Remarksmentioning

confidence: 99%

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Rubella Virus Replication and Links to Teratogenicity

Lee

Bowden

2000

Clinical Microbiology Reviews

152

115

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Section: Mitochondrial Changesmentioning

confidence: 95%

Section: Teratogenicity: Concluding Remarksmentioning

confidence: 99%

Rubella Virus Replication and Links to Teratogenicity

Lee

Bowden

2000

Clinical Microbiology Reviews

152

115

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“…Glycolysis is also influenced by various virus infections [28,29,30,31,32,33]. Ritter et al [31] reported a significant increase in the extracellular release of glucose and lactate and an increase in intracellular metabolite concentrations during the initial steps of the glycolytic pathway in IAV-infected cells from those parameters in the control cells at 12 hpi.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have revealed that mitochondria are responsible for regulating the mammalian innate immune response [20,21], particularly with regard to infection with RNA viruses (including influenza virus) [22,23,24,25,26,27]. Further, the effect of viruses on glycolytic metabolism has also been reported [28,29,30,31,32,33]. …”

Section: Introductionmentioning

confidence: 99%

Hochuekkito, a Japanese Herbal Medicine, Restores Metabolic Homeostasis between Mitochondrial and Glycolytic Pathways Impaired by Influenza A Virus Infection

Takanashi

Dan²,

Kanzaki³

et al. 2017

Pharmacology

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Background: Hochuekkito (HKT), a traditional Japanese herbal medicine (Kampo), has been used to treat symptoms of several diseases. In a recent clinical study, HKT was shown to be protective against the influenza virus infection. However, the underlying mechanism of the prophylactic effect is not clear. Mitochondrial and glycolytic pathways play important roles in cellular energy metabolism to maintain biological functions. These metabolic pathways are affected by the influenza virus infection. In this study, we examined the relationship between the preventive effects of HKT against the influenza virus infection and cellular energy metabolism in mitochondria and glycolysis using Madin-Darby canine kidney cells and influenza A/PR/8/34 (H1N1) virus (IAV). Methods: Mitochondrial and glycolytic metabolic pathways were evaluated on the basis of the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), respectively, using the XF24 Extracellular Analyzer. Results: The OCR/ECAR ratio in IAV-infected cells was lower than that in control cells. Cells that were treated with HKT before IAV infection showed a metabolic pattern similar to that in the control cells (increase in both OCR and ECAR). Conclusions: Our results suggest that HKT not only activates both mitochondrial and glycolytic energy metabolism in IAV-infected cells but also helps maintain metabolic homeostasis similar to that in noninfected cells.

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“…No overall inhibition of protein or RNA synthesis has been obser ved during the first 70 h after infection [2], Vaheri and C ristofalo [3] have shown that an increase of the glycolysis rate, an inhibition of mitosis and macromolecular synthesis took place not earlier than 10 h after infection.…”

mentioning

confidence: 99%

Respiration and ATP Level in BHK21/13S Cells during the Earliest Stages of Rubella Virus Replication

Bardeletti¹

1977

Intervirology

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This study is concerned with the effect of virus infection on the energy balance of the cell and the possible involvement of mitochondria in virus replication. The process of rubella virus adsorption and penetration is immediately associated with a stimulation of O₂ uptake. Shortly afterwards, ATP levels decline, possibly because of increased ATP utilization. Virus infection appears to protect mitochondria against the uncoupling effect of dinitrophenol.

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Metabolism of rubella virus-infected BHK21 cells Enhanced glycolysis and late cellular inhibition

Cited by 22 publications

References 22 publications

Rubella Virus Replication and Links to Teratogenicity

Rubella Virus Replication and Links to Teratogenicity

Hochuekkito, a Japanese Herbal Medicine, Restores Metabolic Homeostasis between Mitochondrial and Glycolytic Pathways Impaired by Influenza A Virus Infection

Respiration and ATP Level in BHK21/13S Cells during the Earliest Stages of Rubella Virus Replication

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