Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development

Abstract

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Extensin arabinoside chain length is modulated in elongating cotton fibre

“…Based on the profile of extractable callose, we recently estimated the transition from primary to secondary cell wall deposition occurs approximately at 17 DPA ( Guo et al, 2019 ). CDTA-extractable extensins displayed a peak coinciding with onset of secondary wall deposition.…”

“…And 3) the side-chains might be degraded by appropriate hydrolases. Expressed arabinofuranosidase candidates of GH3 and GH51 have been reported and biochemical evidence for arabinofuranosidase activity in crude fibre extracts provided ( Guo et al, 2019 ). The activities were implied in pectic arabinan metabolism, which accompany cotton fibre middle lamella degradation.…”

“…30834 and JWF15 are diploid cotton and PimaS7 and FM966 are allopolyploid cotton cultivars. The transcriptomic dataset analysed for transcripts of genes encoding extensin glycosyltransferases are identical to the one analysed previously for transcripts of relevance to the cotton fibre middle lamella metabolism and so is the material analysed by CoMPP ( Guo et al, 2019 ).…”

“…A number at the top of each Y-axis indicates how they are scaled relative to one another. Expression of a xyloglucan fucosyltransferase was displayed with a Y-axis of 1 in ( Guo et al, 2019 ) and these authors regarded this the lowest expression level that could reliably be measured; and this scaling is used also here. Some transcripts that might have been of interest, like SGT1 , fell below this threshold.…”

Experimental Manipulation of Pectin Architecture in the Cell Wall of the Unicellular Charophyte, Penium Margaritaceum

“…Recently, the study of pectins in muro has benefited significantly from the application of analyses of mutants associated with pectin biosynthesis ( Francocci et al., 2013 ; Biswal et al., 2018 ; Wang et al., 2019 ), high resolution microscopy using pectin-specific probes ( Ralet et al., 2010 ; Anderson et al., 2012 ; Mravec et al., 2014 ; Mravec et al., 2017a ; Guo et al., 2019 ; Zhao et al., 2019 ), super resolution three-dimensional direct stochastic optical reconstruction microscopy (3D-dSTORM; Haas et al., 2020 ), atomic force microscopy ( Kirby et al., 2008 ; Paniagua et al., 2014 ; Imaizumi et al., 2017 ), and solid state nuclear magnetic resonance spectroscopy ( Wang et al., 2015 ). These studies have shown that the microarchitecture of pectic polysaccharides in the wall is highly complex and modulates during cell expansion, development and in response to external abiotic and biotic stress.…”