Experimental Manipulation of Pectin Architecture in the Cell Wall of the Unicellular Charophyte, Penium Margaritaceum

Palacio‐López, Kattia; Sun, Li; Reed, Reagan; Kang, Eunchai; Sørensen, Iben; Rose, Jocelyn K. C.; Domozych, David S.

doi:10.3389/fpls.2020.01032

Cited by 20 publications

(6 citation statements)

References 72 publications

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“…The possible inferences that could be drawn from these observations are that during the process of extraction, a certain loss of crystallinity comes into picture. It could be postulated that by subjecting the molecule to stresses encountered during extraction condition can cause change in molecule lattice exposing some of the components in an irregular manner such that an ordered structure is lost, which can possess more crystallinity otherwise (Palacio‐Lopez et al ., 2020). Reduction in crystalline nature due to extraction is because of hydrolysis of crystalline areas (Rahimi & Behrooz, 2011).…”

Section: Resultsmentioning

confidence: 99%

Pectin recovery from apple pomace: physico‐chemical and functional variation based on methyl‐esterification

Naqash

Masoodi

Gani

et al. 2021

Int J of Food Sci Tech

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Summary This study explored the valorisation of apple pomace by extracting pectin with both high and low degrees of esterification (DM). Two types of pectin were extracted with a DM of 43.29% being low methoxyl (LM) pectic polysaccharide and high methoxyl (HM) pectin with a DM of 65.88%. HM pectin was characterised by a WHC and OHC of 1.3 g g−1 and 0.4 g g−1, respectively, compared to 0.32 g g−1 and 0.14 g g−1, respectively, for LM pectic polysaccharide. HM pectin possessed greater purity indicated by 69.18% GalA, whereas LM pectic polysaccharide possessed 41.26% GalA. HM pectin revealed better functionality in terms of high emulsifying activity which was concentration‐dependent, and emulsion stability than LM pectic polysaccharide. The solubility of HM pectin was 90.8%, which is preferable for various industrial applications. LM pectic polysaccharide was characterised by a higher % radical‐scavenging activity in a concentration‐dependent manner. XRD analysis revealed LM pectic polysaccharide to possess higher crystallinity (53%) and SEM analysis revealed its surface to be rough and coarse.

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Section: Resultsmentioning

confidence: 99%

Pectin recovery from apple pomace: physico‐chemical and functional variation based on methyl‐esterification

Naqash

Masoodi

Gani

et al. 2021

Int J of Food Sci Tech

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“…2, F and G, and figs. S7 and S8) ( 26 , 27 ). This observed architecture is reminiscent of previously described ring like pectin bands in pollen tubes ( 28 , 29 ).…”

Section: Lrx8 Ralf4 and Pectin Form A Reticulated Pattern In Vivomentioning

confidence: 99%

Plant cell wall patterning and expansion mediated by protein-peptide-polysaccharide interaction

Moussu,

Lee,

Haas

et al. 2023

Science

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Assembly of cell wall polysaccharides into specific patterns is required for plant growth. A complex of RAPID ALKALINIZATION FACTOR 4 (RALF4) and its cell wall–anchored LEUCINE-RICH REPEAT EXTENSIN 8 (LRX8)–interacting protein is crucial for cell wall integrity during pollen tube growth, but its molecular connection with the cell wall is unknown. Here, we show that LRX8-RALF4 complexes adopt a heterotetrametric configuration in vivo, displaying a dendritic distribution. The LRX8-RALF4 complex specifically interacts with demethylesterified pectins in a charge-dependent manner through RALF4’s polycationic surface. The LRX8-RALF4-pectin interaction exerts a condensing effect, patterning the cell wall’s polymers into a reticulated network essential for wall integrity and expansion. Our work uncovers a dual structural and signaling role for RALF4 in pollen tube growth and in the assembly of complex extracellular polymers.

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“…The same plants not only express high levels of OG-oxidases but also an oxidase acting on CDs (Locci et al, 2019). Notably, recent studies show that cellulose and pectin interact (Du et al, 2020;Palacio-Lopez et al, 2020) and that cellulose microfibers and HG nanofilaments form a single cohesive network (Haas et al, 2020;Zhang and Zhang, 2020).…”

Section: Final Considerations: the Role Of Oxidases In The Maintenancmentioning

confidence: 99%

Dampening the DAMPs: How Plants Maintain the Homeostasis of Cell Wall Molecular Patterns and Avoid Hyper-Immunity

et al. 2020

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Several oligosaccharide fragments derived from plant cell walls activate plant immunity and behave as typical damage-associated molecular patterns (DAMPs). Some of them also behave as negative regulators of growth and development, and due to their antithetic effect on immunity and growth, their concentrations, activity, time of formation, and localization is critical for the so-called “growth-defense trade-off.” Moreover, like in animals, over accumulation of DAMPs in plants provokes deleterious physiological effects and may cause hyper-immunity if the cellular mechanisms controlling their homeostasis fail. Recently, a mechanism has been discovered that controls the activity of two well-known plant DAMPs, oligogalacturonides (OGs), released upon hydrolysis of homogalacturonan (HG), and cellodextrins (CDs), products of cellulose breakdown. The potential homeostatic mechanism involves specific oxidases belonging to the family of berberine bridge enzyme-like (BBE-like) proteins. Oxidation of OGs and CDs not only inactivates their DAMP activity, but also makes them a significantly less desirable food source for microbial pathogens. The evidence that oxidation and inactivation of OGs and CDs may be a general strategy of plants for controlling the homeostasis of DAMPs is discussed. The possibility exists of discovering additional oxidative and/or inactivating enzymes targeting other DAMP molecules both in the plant and in animal kingdoms.

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Experimental Manipulation of Pectin Architecture in the Cell Wall of the Unicellular Charophyte, Penium Margaritaceum

Cited by 20 publications

References 72 publications

Pectin recovery from apple pomace: physico‐chemical and functional variation based on methyl‐esterification

Pectin recovery from apple pomace: physico‐chemical and functional variation based on methyl‐esterification

Plant cell wall patterning and expansion mediated by protein-peptide-polysaccharide interaction

Dampening the DAMPs: How Plants Maintain the Homeostasis of Cell Wall Molecular Patterns and Avoid Hyper-Immunity

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