The relationship between octanol/water partition coefficient (KO,) and bioconcentration factor (BCF) of 14 monochloro-to pentachloronitrobenzenes were examined in rainbow trout through waterborne and dietary exposure studies. The results indicated BCF was not significantly correlated with KO, for this chemical group. The role of chemical half-lives and chemical absorption efficiencies are examined as factors that could explain why the BCF of some chemical groups are lower than predicted.