Metabolism of nitazoxanide in rats, pigs, and chickens: Application of liquid chromatography coupled to hybrid linear ion trap/Orbitrap mass spectrometer

Huang, Xianhui; Guo, Chunna; Zhang-liu, Chen; Liu, Yahong; He, Lili; Zeng, Zhenling; Yan, Chaoqun; Pan, Guangfang; Li, Shuaipeng

doi:10.1016/j.jchromb.2015.05.025

Cited by 11 publications

(13 citation statements)

References 23 publications

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“…A similar phenomenon has been found in metabolism of nitazoxanide in pigs and chicken. The conjugated metabolites of nitazoxanide are predominantly glucuronide in pigs but sulfate in chickens (Huang et al, 2015). There is limited information of age-dependent phase II metabolism of drugs in pigs or chicken.…”

Section: Discussionmentioning

confidence: 99%

“…Hepatic o-aminophenyl glucuronidation activity rapidly increases posthatch in a domestic fowl from virtually zero in chick embryo livers to its adult values (Dutton & Ko, 1966). Its activity in embryonic and adult chicken livers is induced by phenobarbital, hexabromocyclododecane, and polychlorinated biphenyls (Roth & Levine, 1983;van den Hurk et al, 2007;Crump et al, 2010). The mean hepatic activity of 4-nitrophenol glucuronidation in male Sasso Broiler chickens increases from 3 to 12 weeks posthatch (Coulet et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Hepatic mRNA expression of most SULT isoforms in mice is low in fetal livers, reaches its highest expression around 15-22 days, and then reduces to their adult level (Alnouti & Klaassen, 2006;Kocarek et al, 2008). Information regarding developmental changes of SULT is limited in livestock, although SULT activity has been detected in chickens (van den Hurk et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Age‐related change of hepatic uridine diphosphate glucuronosyltransferase and sulfotransferase activities in male chickens and pigs

2016

Vet Pharm & Therapeutics

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The hepatic activities of uridine diphosphate glucuronosyltransferase (UGT) and sulfotransferase (SULT) of male Ross 708 broiler chickens at the age of 1, 7, 14, 28, and 56 days and male Camborough-29 pigs at the age of 1 day and 2, 5, 10, and 20 weeks were investigated. Glucuronidation and sulfation of 4-nitrophenol were used to evaluate the activities. Porcine hepatic UGT and SULT activities were low at birth, peaked at around 5-10 weeks, and then declined. Both hepatic UGT and SULT activities of chickens were high at hatch and declined. Chicken hepatic UGT activity had a peak at the age of 28 days. Affinity of hepatic SULT to 4-nitrophenol is similar in chickens and pigs, but the affinity of hepatic UGT in pigs was about 10 times higher than that in chickens. 4-nitrophenol was predominantly conjugated by SULT instead of UGT in chicken livers from hatch to day 56. Conversely, hepatic UGT contributed predominantly in 4-nitrophenol conjugation than the SULT in pigs from birth to 20 weeks. Therefore, age has significant impact on hepatic activities of UGT and SULT, and the importance of UGT and SULT on conjugation is different in chickens and pigs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Age‐related change of hepatic uridine diphosphate glucuronosyltransferase and sulfotransferase activities in male chickens and pigs

2016

Vet Pharm & Therapeutics

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“…Tizoxanide glucuronide (TG, Fig. 1C) has been identified as the main metabolite in plasma, urine and bile (Huang et al, 2015). Thus, it is necessary to monitor the plasma concentration-time curves of T and TG in clinical and preclinical pharmacokinetic studies.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of tizoxanide and tizoxanide glucuronide in mouse plasma by liquid chromatography–tandem mass spectrometry

Jin-qiu

Meng²,

et al. 2016

Biomedical Chromatography

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Nitazoxanide (NTZ) is a broad-spectrum antimicrobial agent. Tizoxanide (T) and tizoxanide glucuronide (TG) are the major circulating metabolites after oral administration of NTZ. A rapid and specific LC-MS/MS method for the simultaneous quantification of T and TG in mouse plasma was developed and validated. A simple acetonitrile-induced protein precipitation method was employed to extract two analytes and the internal standard glipizide from 50 μL of mouse plasma. The purified samples were resolved using a C column with a mobile phase consisting of acetonitrile and 5 mm ammonium formate buffer (containing 0.05% formic acid) following a gradient elution. An API 3000 triple quadrupole mass spectrometer was operated under multiple reaction-monitoring mode with electrospray ionization. The precursor-to-product ion transitions m/z 264 → m/z 217 for T and m/z 440 → m/z 264 for TG were used for quantification. The developed method was linear in the concentration ranges of 1.0-500.0 ng/mL for T and 5.0-1000.0 ng/mL for TG. The intra- and inter-day precision and accuracy of the quality control samples at low, medium and high concentrations exhibited an RSD of <13.2% and the accuracy values ranged from -9.6 to 9.3%. We used this validated method to study the pharmacokinetics of T and TG in mice following oral administration of NTZ. Copyright © 2016 John Wiley & Sons, Ltd.

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“…Orbitrap with its resolution of resolving power in excess of 1,000,000 FWHM excels in qualitative analyses and is widely used for metabolite identification (Herrero, Borrull, Pocurull, & Marce, 2014;Vallverdu-Queralt et al, 2015). LTQ-Orbitrap, containing a Orbitrap and a linear ion trap, combines both qualitative and quantitative analysis and is widely used in metabolism research (Farre, Pico, & Barcelo, 2014;Huang et al, 2015;Lopez-Gutierrez, Romero-Gonzalez, Plaza-Bolanos, Vidal, & Frenich, 2015). An ultra-high-pressure liquid chromatography (UHPLC)-LTQ-Orbitrap mass spectrometer acquired full-scan MS data for quantification, and data-dependent (dd) MS 2 and MS 3 product ion spectra for identification.…”

mentioning

confidence: 99%

Simultaneous quantification and semi‐quantification of amentoflavone and its metabolites in human intestinal bacteria by liquid chromatography Orbitrap high‐resolution mass spectrometry

Qian

Jiang

Zhu

et al. 2017

Biomedical Chromatography

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A quick, easy, effective method followed by ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap MS) was developed for the simultaneous identification and quantification of the metabolites produced by amentoflavone (AMF) in human intestinal bacteria from human feces. The method validated for quantification of AMF concerning precision, accuracy, recovery, matrix effect, stability and limits showed acceptable results. Compared with blank human intestinal bacteria chromatography, three metabolites were identified based on high-accuracy protonated precursors and multi-stage mass spectrometry (MS ) using the proposed strategy. At the same time, a new method was developed for semi-quantification of three metabolites. We describe the trend over 24 h of concentration-time curves for AMF and its metabolites. Moreover, the main metabolic pathway of AMF was clarified in human intestinal bacteria. The method was validated and successfully applied to the detection and quantification of AMF and its metabolites.

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Metabolism of nitazoxanide in rats, pigs, and chickens: Application of liquid chromatography coupled to hybrid linear ion trap/Orbitrap mass spectrometer

Cited by 11 publications

References 23 publications

Age‐related change of hepatic uridine diphosphate glucuronosyltransferase and sulfotransferase activities in male chickens and pigs

Age‐related change of hepatic uridine diphosphate glucuronosyltransferase and sulfotransferase activities in male chickens and pigs

Simultaneous quantification of tizoxanide and tizoxanide glucuronide in mouse plasma by liquid chromatography–tandem mass spectrometry

Simultaneous quantification and semi‐quantification of amentoflavone and its metabolites in human intestinal bacteria by liquid chromatography Orbitrap high‐resolution mass spectrometry

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