Age‐related change of hepatic uridine diphosphate glucuronosyltransferase and sulfotransferase activities in male chickens and pigs

Hu, Steve

doi:10.1111/jvp.12355

Cited by 9 publications

(8 citation statements)

References 67 publications

(101 reference statements)

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“…Age has significant impact on hepatic activities of glucuronidation and sulfation. For example, porcine hepatic glucuronidation and sulfation activities were low at birth, peaked at 5–10 weeks, and then declined at 20 weeks (Hu, 2017), similar to the observations in the present study.…”

Section: Discussionsupporting

confidence: 89%

Age-associated changes of cytochrome P450 and related phase-2 gene/proteins in livers of rats

Xu¹,

Hu²,

Xie³

et al. 2019

PeerJ

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Cytochrome P450s (CYPs) are phase-I metabolic enzymes playing important roles in drug metabolism, dietary chemicals and endogenous molecules. Age is a key factor influencing P450s expression. Thus, age-related changes of CYP 1–4 families and bile acid homeostasis-related CYPs, the corresponding nuclear receptors and a few phase-II genes were examined. Livers from male Sprague-Dawley rats at fetus (−2 d), neonates (1, 7, and 14 d), weanling (21 d), puberty (28 and 35 d), adulthood (60 and 180 d), and aging (540 and 800 d) were collected and subjected to qPCR analysis. Liver proteins from 14, 28, 60, 180, 540 and 800 days of age were also extracted for selected protein analysis by western blot. In general, there were three patterns of their expression: Some of the drug-metabolizing enzymes and related nuclear receptors were low in fetal and neonatal stage, increased with liver maturation and decreased quickly at aging (AhR, Cyp1a1, Cyp2b1, Cyp2b2, Cyp3a1, Cyp3a2, Ugt1a2); the majority of P450s (Cyp1a2, Cyp2c6, Cyp2c11, Cyp2d2, Cyp2e1, CAR, PXR, FXR, Cyp7a1, Cyp7b1. Cyp8b1, Cyp27a1, Ugt1a1, Sult1a1, Sult1a2) maintained relatively high levels throughout the adulthood, and decreased at 800 days of age; and some had an early peak between 7 and 14 days (CAR, PXR, PPARα, Cyp4a1, Ugt1a2). The protein expression of CYP1A2, CYP2B1, CYP2E1, CYP3A1, CYP4A1, and CYP7A1 corresponded the trend of mRNA changes. In summary, this study characterized three expression patterns of 16 CYPs, five nuclear receptors, and four phase-II genes during development and aging in rat liver, adding to our understanding of age-related CYP expression changes and age-related disorders.

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Section: Discussionsupporting

confidence: 89%

Age-associated changes of cytochrome P450 and related phase-2 gene/proteins in livers of rats

Xu¹,

Hu²,

Xie³

et al. 2019

PeerJ

View full text Add to dashboard Cite

show abstract

“…Age has significant impact on hepatic activities of glucuronidation and sulfation. For example, porcine hepatic glucuronidation and sulfation activities were low at birth, peaked at 5-10 weeks, and then declined at weeks, (Hu 2017) similar to the observations in the present study.…”

Section: Ugt and Sultsupporting

confidence: 90%

Age-associated changes of cytochrome P450 and related phase-2 gene/proteins in livers of rats

Xie

et al. 2019

Preprint

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Cytochrome P450s (CYPs) are phase-I metabolic enzymes playing important roles in drug metabolism, dietary chemicals and endogenous molecules. Age is a key factor influencing P450s expression. Thus, age-related changes of CYP 1-4 families and bile acid homeostasis-related CYPs, the corresponding nuclear receptors and a few phase-II genes were examined. Livers from male Sprague-Dawley rats at fetus (-2 d), neonates (1, 7, and 14 d), weanling (21 d), puberty (28 and 35 d), adulthood (60 and 180 d), and aging (540 and 800 d) were collected and subjected to qPCR analysis. Liver proteins from 14, 28, 60, 180, 540 and 800 days of age were also extracted for selected protein analysis by Western-blot. In general, there were three patterns of their expression: Some of the drug-metabolizing enzymes and related nuclear receptors were low in fetal and neonatal stage, increased with liver maturation and decreased quickly at aging (AhR, Cyp1a1, Cyp2b1, Cyp2b2, Cyp3a1, Cyp3a2, Ugt1a2); the majority of P450s (Cyp1a2, Cyp2c6, Cyp2c11, Cyp2d2, Cyp2e1, CAR, PXR, FXR, Cyp7a1, Cyp7b1. Cyp8b1, Cyp27a1, Ugt1a1, Sult1a1, Sult1a2) maintained relatively high levels throughout the adulthood, and decreased at 800 days of age; and some had an early peak between 7 and 14 days (CAR, PXR, PPARα, Cyp4a1, Ugt1a2). The protein expression of CYP1A2, CYP2B1, CYP2E1, CYP3A1, CYP4A1, and CYP7A1 corresponded the trend of mRNA changes. In summary, this study characterized three expression patterns of 16 CYPs, 5 nuclear receptors, and 4 phase-II genes during development and aging in rat liver, adding to our understanding of age-related CYP expression changes and age-related disorders.

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“…Similarly, low hepatic levels of UGT enzymes have described in fetuses and newborn sheep [ 38 ]. Recently, significant age-dependent hepatic activities of UGT were reported for pigs [ 39 ], with glucuronidation achieving peak activity at approximately 5–10 weeks after birth. The piglets after intrauterine DON exposure in our experiment were able to eliminate DON from their blood between 7 and 14 weeks.…”

Section: Discussionmentioning

confidence: 99%

Maternal Exposure Results in Long-Term Deoxynivalenol Persistence in Piglets’ Plasma and Modulates the Immune System

Štěpánová

Hlavová

Šťastný

et al. 2020

Toxins

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Deoxynivalenol (DON)-contaminated feed represents a serious problem for pigs due to their high sensitivity to its toxicological effects. The aim of the present study was to evaluate the impact of intrauterine DON exposure on the immune system of piglets. Pure DON was intravenously administered to sows at the end of gestation (during the last 2–3 days of gestation, one dose of 300 µg per day). The plasma concentration of DON was analyzed using liquid chromatography combined with high-resolution Orbitrap-based mass spectrometry (LC–MS/MS (HR)) and selected immune parameters were monitored six times in piglets from birth to 18 weeks. DON was found in the plasma of 90% of newborn piglets at a mean concentration of 6.28 ng/mL and subsequently, at one, three, and seven weeks after birth with decreasing concentrations. Trace amounts were still present in the plasma 14 weeks after birth. Flow cytometry revealed a significant impact of DON on T lymphocyte subpopulations during the early postnatal period. Lower percentages of regulatory T cells, T helper lymphocytes, and their double positive CD4+CD8+ subset were followed by increased percentages of cytotoxic T lymphocytes and γδ T cells. The capacity to produce pro-inflammatory cytokines was also significantly lower after intrauterine DON exposure. In conclusion, this study revealed a long-term persistence of DON in the plasma of the piglets as a consequence of short-term intrauterine exposure, leading to altered immune parameters.

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Age‐related change of hepatic uridine diphosphate glucuronosyltransferase and sulfotransferase activities in male chickens and pigs

Cited by 9 publications

References 67 publications

Age-associated changes of cytochrome P450 and related phase-2 gene/proteins in livers of rats

Age-associated changes of cytochrome P450 and related phase-2 gene/proteins in livers of rats

Age-associated changes of cytochrome P450 and related phase-2 gene/proteins in livers of rats

Maternal Exposure Results in Long-Term Deoxynivalenol Persistence in Piglets’ Plasma and Modulates the Immune System

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