Metabolism of fensulfothion by a soil bacterium, Pseudomonas alcaligenes C1

Sheela, S.; Pai, S.Balakrishna

doi:10.1128/aem.46.2.475-479.1983

Cited by 13 publications

(5 citation statements)

References 10 publications

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“…The effect of various substrates on the microbial degradation of organic pollutants has been the subject of a number of studies (3,15,23,26,30,34,36,37), many of which used pseudomonads. Pseudomonads have demonstrated the ability to degrade a wide variety of aromatic compounds (6,20,21,23,27,35,38). However, utilization of these compounds by members of the genus Pseudomonas apparently can be altered by the presence of more easily metabolized compounds, such as acetate (23,34), glucose (26,30), pyruvate (27), arginine (30), and unidentified dissolved organic carbon (34).…”

mentioning

confidence: 99%

Solid-state 13C nuclear magnetic resonance spectroscopy of simultaneously metabolized acetate and phenol in a soil Pseudomonas sp

Heiman¹,

Cooper²

1987

Appl Environ Microbiol

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We investigated concentration-dependent primary and secondary substrate relationships in the simultaneous metabolism of the ubiquitous pollutant phenol and the naturally occurring substrate acetate by a Pseudomonas sp. soil isolate capable of utilizing either substance as a sole source of carbon and energy. In addition to conventional analytical techniques, solid-state 13C nuclear magnetic resonance spectroscopy was used to follow the cellular distribution of [1-_3C]acetate in the presence of unlabeled phenol. With 5 mM acetate as the primary substrate, Pseudomonas sp. 9S8D2 removed 1 mM phenol (secondary substrate) at a rate of 2 nmol/mg of total cell protein. Although extensive acetate metabolism was indicated by a significant redistribution of the carboxyl label, this redistribution was not affected by the presence of phenol as a secondary substrate. When the primary and secondary substrate roles were reversed, however, the presence of 1 mM phenol altered the metabolism of 0.1 mM acetate, as evidenced by both the two-to fourfold increases in carboxyl label that appeared in terminal methyl and acyl chain methylene carbon resonances and the decrease in label that occurred in the carbohydrate spectral region. These results suggest that, when phenol is present as the primary substrate, acetate is preferentially shuttled into fatty acyl chain synthesis, whereas phenol carbon is funnelled into the tricarboxylic acid cycle. Thus, simultaneous use of a xenobiotic compound and a natural substrate apparently does occur, and the relative concentrations of the two substrates do influence the rate and manner in which the compounds are utilized. These results also demonstrate the unique advantage of using solid-state nuclear magnetic resonance techniques combined with 13C labeling of specific sites in substrates when doing microbial degradation studies. In this work, the entire cellular biomass was examined directly without extensive extraction, fractionation, or isolation of subcellular units; thus, there is no uncertainty about chemical alteration of substrate metabolites as a result of these often harsh treatments.

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confidence: 99%

Solid-state 13C nuclear magnetic resonance spectroscopy of simultaneously metabolized acetate and phenol in a soil Pseudomonas sp

Heiman¹,

Cooper²

1987

Appl Environ Microbiol

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“…Pseudomonas spp. have an ability to degrade a wide variety of aromatic compounds, e.g., chlorocatechols (16), 4-chloro-2-methylphenoxyacetate (10), 4-chlorophenoxyacetate (9), fensulfothion (26), and naphthalenesulfonic acids (5). All of these compounds must be regarded as hazardous to the environment, and they are resistant to biological degradation.…”

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confidence: 99%

Degradation of phenol by Pseudomonas putida ATCC 11172 in continuous culture at different ratios of biofilm surface to culture volume

Molin¹,

Nilsson²

1985

Appl Environ Microbiol

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Pseudomonas putida ATCC 11172 was grown in continuous culture with phenol as the only carbon and energy source; a culture practically without biofilm was compared with biofilm cultures of differing surface area/volume ratios. The biofilm did not significantly affect the maximal suspended cell concentration in the effluent, but it increased the maximal phenol reduction rate from 0.23 g/liter per h (without biofilm) to 0.72 g/liter per h at the highest biofilm level (5.5 cm2 of biofilm surface per ml of reactor volume). The increase in phenol reduction rate was linear up to the surface area/volume ratio of 1.4 cm2/ml. The continuous cultures with biofilms' could tolerate a higher phenol concentration of the medium (3.0 g/liter) than the nonbiofilm system (2.5 g/liter). At higher dilution rates an intermediate product, 2-hydroxymuconic semialdehyde, accumulated in the culture. When the biomass of the effluent started to decrease, the concentration of 2-hydroxymuconic semialdehyde reached a peak value. We conclude that biofilms in continuous culture have the potential to enhance the aerobic degradation of aromatic compounds.

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“…Timely detection of an increased concentration of pesticides in the soil allows for measures to reduce their concentration. Currently, it is proposed achieve this by biological means of protection, such as, for example, the enzymatic hydrolysis of organophosphorus compounds in the soil [30] or the application of exogenous organic matter [31].…”

Section: Introductionmentioning

confidence: 99%

“…Timely detection of an increased content of pesticide in the soil will allow farm producers to reduce the negative impact on the environment and yield. For example, a method of enzymatic hydrolysis of organophosphorus compounds in the soil was proposed [30]. At the same time, a biocatalyst is introduced into the soil-an uncleaned polyhistidine-containing polypeptide with the properties of organophosphate hydrolase, obtained from a strain of Escherichia coli bacteria CCMIBX 29 and immobilized on a cellulose-containing carrier.…”

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confidence: 99%

Improvement of the Integral Indicator of the Ecological and Toxicological Assessment of the Danger of the Use of Pesticides in Agriculture and the Creation of an Information System for Their Monitoring

Slastya,

Khudyakova,

Vasenev

et al. 2023

Agriculture

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The aim of the work was to assess the ecological and toxicological dangers of pesticides using the value of a complex indicator determined by the calculation method. An integral indicator of the relative ecological and toxicological danger of pesticide use (Hr) is proposed, which allows taking into account the acute oral and chronic toxicity of the pesticide for mammals and its impact on the environment (half-life in soil and chronic toxicity for aquatic organisms), as well as the rate of application of the drug. The computation was performed on fungicides and insecticides that are most commonly used in grain crop cultivation and approved to be applied in the Russian Federation. The research was carried out in 2022–2023. The results showed that the determined indicator takes values from 0.02 for the fungicide benomyl to 26950 for the insecticide chlorpyrifos. Pesticides were ranked according to the Hr index, and four hazard groups were identified, as well as the main factors that determine them. The first hazard group should include drugs with a Hr greater than 1000; the second hazard group—with Hr from 100 to 1000; the third—with Hr from 1 to 100; and the fourth—with Hr less than 1. The first group includes pesticides with multiple adverse safety profiles, such as chlorpyrifos. The second group includes insecticides fipronil, lambda-cyhalothrin, gamma-cyhalothrin, imidacloprid and the fungicide flutriafol. The indicator can be used for agro-ecological substantiation of the choice of pesticides for the treatment of agricultural crops and for the selection of priority pesticides for regular monitoring of their content in the environment—primarily the first and second hazard groups. It can be performed remotely by appropriate detectors and sensors. All data about farm pollution can be monitored using an information server monitoring system, the architecture of which has been proposed.

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Metabolism of fensulfothion by a soil bacterium, Pseudomonas alcaligenes C1

Cited by 13 publications

References 10 publications

Solid-state 13C nuclear magnetic resonance spectroscopy of simultaneously metabolized acetate and phenol in a soil Pseudomonas sp

Solid-state 13C nuclear magnetic resonance spectroscopy of simultaneously metabolized acetate and phenol in a soil Pseudomonas sp

Degradation of phenol by Pseudomonas putida ATCC 11172 in continuous culture at different ratios of biofilm surface to culture volume

Improvement of the Integral Indicator of the Ecological and Toxicological Assessment of the Danger of the Use of Pesticides in Agriculture and the Creation of an Information System for Their Monitoring

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