Degradation of phenol by Pseudomonas putida ATCC 11172 in continuous culture at different ratios of biofilm surface to culture volume

Molin, Göran; Nilsson, Inge

doi:10.1128/aem.50.4.946-950.1985

Cited by 45 publications

(10 citation statements)

References 27 publications

(25 reference statements)

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“…A more accurate value could not be obtained because of limitations in the sensitivity of the method at low phenol concentrations. Less precise K, values determined from carbon-limited continuous cultures of several Pseudomonas species and Trichosporon cutaneum ranged from less than 10 to about 30 ,uM (4,10,18). Inhibition of phenol degradation 10 aC) Rates of disappearance were determined from eight sets of duplicate samples over 4 h for initial TCE concentrations (5 to 50 FiM).…”

Section: Resultsmentioning

confidence: 98%

Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates

Folsom

Chapman

Pritchard

1990

Appl Environ Microbiol

306

113

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Intact cells of Pseudomonas cepacia G4 completely degraded trichloroethylene (TCE) following growth with phenol. Degradation kinetics were determined for both phenol, used to induce requisite enzymes, and TCE, the target substrate. Apparent Ks and Vma. values for degradation of phenol by cells were 8.5 ,LM and 466 nmol/min per mg of protein, respectively. At phenol concentrations greater than 50 ,uM, phenol degradation was inhibited, yielding an apparent second-order inhibitory value, Ks,, of 0.45 mM as modeled by the Haldane expression. A partition coefficient for TCE was determined to be 0.40 0.02, [TCEair]/[TCEwartr, consistent with Henry's law. To eliminate experimental problems associated with TCE volatility and partitioning, a no-headspace bottle assay was developed, allowing for direct and accurate determinations of aqueous TCE concentration. By this assay procedure, apparent Ks and Vm, values determined for TCE degradation by intact cells were 3 ,uM and 8 nmol/min per mg of protein, respectively. Following a transient lag period, P. cepacia G4 degraded TCE at concentrations of at least 300 ,uM with no apparent retardation in rate. Consistent with K3 values determined for degradation, TCE significantly inhibited phenol degradation.

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Section: Resultsmentioning

confidence: 98%

Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates

Folsom

Chapman

Pritchard

1990

Appl Environ Microbiol

306

113

View full text Add to dashboard Cite

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“…We observed that these strains likely issued from the phyllosphere; P. graminis P. syringae, P. fluorescence, P. poae, and P. viriflava were able to degrade phenol. In the literature, P. aeruginosa and P. putida are the most popular phenol degraders (Basha et al, 2010;Der Yang and Humphrey, 1975;Erhan et al, 2004;Gami et al, 2014;Kumar et al, 2005;Molin and Nilsson, 1985). Interestingly, Bartoli et al (2015) showed that the genome of several P. syringae pathogens of woody plants contained a catechol operon, while it was not the case for other P. syringae strain pathogens of herbaceous plants.…”

Section: Discussionmentioning

confidence: 99%

Potential for phenol biodegradation in cloud waters

et al. 2018

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Abstract. Phenol is toxic and can be found in many environments, in particular in the atmosphere due to its high volatility. It can be emitted directly from manufacturing processes or natural sources, and it can also result from benzene oxidation. Although phenol biodegradation by microorganisms has been studied in many environments, the cloud medium has not been investigated yet as the discovery of active microorganisms in cloud is rather recent. The main objective of this work was to evaluate the potential degradation of phenol by cloud microorganisms. Phenol concentrations were measured by GC-MS on two cloud samples collected at the PUY station (summit of Puy de Dôme, 1465 m a.s.l., France): they ranged from 0.15 to 0.21 µg L−1. The strategy for investigating its potential biodegradation involved a metatranscriptomic analysis and metabolic screening of bacterial strains from cloud water collected at the PUY station for phenol degradation capabilities (from the 145 tested strains, 33 were isolated for this work). Among prokaryotic messenger RNA-enriched metatranscriptomes obtained from three cloud water samples, which were different from those used for phenol quantification, we detected transcripts of genes coding for enzymes involved in phenol degradation (phenol monooxygenases and phenol hydroxylases) and its main degradation product, catechol (catechol 1,2-dioxygenases). These enzymes were likely from Gammaproteobacteria, a dominant class in clouds, more specifically the genera Acinetobacter and Pseudomonas. Bacterial isolates from cloud water samples (Pseudomonas spp., Rhodococcus spp., and strains from the Moraxellaceae family) were screened for their ability to degrade phenol: 93 % of the 145 strains tested were positive. These findings highlight the possibility of phenol degradation by microorganisms in clouds. Metatranscriptomic analysis suggested that phenol could be biodegraded in clouds, while 93 % of 145 bacterial strains isolated from clouds were able to degrade phenol.

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“…A number of studies have been instrumental in characterizing the genes encoding phenol dissimilation and in determining how these genes function in pure culture (8,9,11). However, our study is the first report of the expression of the dmpN gene under conditions representative of a mixed-culture environment.…”

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confidence: 95%

Application of reverse transcriptase PCR for monitoring expression of the catabolic dmpN gene in a phenol-degrading sequencing batch reactor

Selvaratnam

Schoedel

McFarland

et al. 1995

Appl Environ Microbiol

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A modified freeze-thaw method in combination with reverse transcriptase PCR was developed for monitoring gene expression in activated sludge. The sensitivity of the methodology was determined by inoculating nonsterile activated sludge samples with 3-chlorobenzoate-degrading Pseudomonas putida PPO301(pRO103), which contains the catabolic tfdB gene. tfdB mRNA was detected in 10 mg of activated sludge inoculated with 10 4 CFU of the target organism. This technique was subsequently utilized to analyze the in situ expression of the catabolic dmpN gene in a sequencing batch reactor (SBR) bioaugmented with phenol-degrading P. putida ATCC 11172. Greatest dmpN expression was observed 15 min after maximum phenol concentration was reached in the reactor and 15 min after the start of aeration. Decreased phenol concentrations in the reactor corresponded to reduced levels of dmpN expression, although low levels of dmpN mRNA were observed throughout the SBR cycle. These results indicate that concentration of phenol in the reactor and the onset of aeration stimulated transcriptional activity of the dmpN gene. The information obtained from this study can be used to alter SBR operational strategies so as to lead to more effective bioaugmentation practices.

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Degradation of phenol by Pseudomonas putida ATCC 11172 in continuous culture at different ratios of biofilm surface to culture volume

Cited by 45 publications

References 27 publications

Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates

Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates

Potential for phenol biodegradation in cloud waters

Application of reverse transcriptase PCR for monitoring expression of the catabolic dmpN gene in a phenol-degrading sequencing batch reactor

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