Metabolism of arachidonic acid by isolated rabbit ciliary epithelium

Constitutive Expression and Localization of COX-1 and COX-2 in Rabbit Iris and Ciliary Body

“…The epithelial layers of both the iris and the ciliary body including its processes do not react for COX-1 or COX-2 antibodies (Figs 3±5).This is in contrast to our results in man (Maiho Èfner et al, 2000), showing COX-1 and -2 in the non-pigmented epithelial layer, but is in line with other observations in rabbits by King et al (1992). These authors have separated rabbit ciliary epithelium from iridial and ciliary stroma, homogenized and incubated both layers in vitro and observed the production of lipoxygenase, but not cyclooxygenase products in the epithelial preparations; the production of both metabolites of arachidonic acid was demonstrated in iris/ciliary stroma.…”

“…These authors have separated rabbit ciliary epithelium from iridial and ciliary stroma, homogenized and incubated both layers in vitro and observed the production of lipoxygenase, but not cyclooxygenase products in the epithelial preparations; the production of both metabolites of arachidonic acid was demonstrated in iris/ciliary stroma. We show a lack of COX production in ciliary epithelial cells of the rabbit as an explanation for the ®ndings of King et al (1992). This disparity of COX production and prostaglandin synthesis between man (Maiho Èfner et al, 2000) and rabbit is interesting, because a strong species variation has been observed in the reaction and anatomy of the eye between man and rabbit.…”

A Second Generation Prostanoid Receptor Antagonist Acting at Multiple Receptor Subtypes

“…Thus, in the arachidonic acid induced uveitis model in rabbits, the prostanoid mediated inflammatory response may be formed in a very discrete fashion. 13 This permitted the effectiveness of prostanoid biosynthesis inhibition and blockade of multiple target prostanoid receptors to be definitively compared. Graded doses of AGN 225660 (0.1%, 0.3%), 0.4% ketorolac, and a combination of 0.3% doses of BW A868C, SC-51322, GW627368X, AS604872, and SQ 29548 were examined in this model.…”

“…In the rabbit eye, arachidonic acid is converted to pro-inflammatory prostaglandins. Thus, in the arachidonic acid induced uveitis model in rabbits, the prostanoid mediated inflammatory response may be formed in a very discrete fashion . This permitted the effectiveness of prostanoid biosynthesis inhibition and blockade of multiple target prostanoid receptors to be definitively compared.…”

Role of protein kinase C in regulation of Na+- and K+-dependent ATPase activity and pump function in corneal endothelial cells

“…Both the release of arachidonic acid from phospholipids catalyzed by phospholipase A 2 and the subsequent metabolism of arachidonic acid are promoted by the phorbol ester-induced activation of PKC in various cell types. [21][22][23] Metabolism of arachidonic acid generates a wide range of products, including prostaglandin E 2 , prostaglandin F 2α , 5-hydroxyeicosatetraenoic acid (5-HETE), and 12-HETE, 24 and these metabolites infl uence cell function. 20 Such arachidonic acid metabolites thus inhibit Na,K-ATPase activity [25][26][27] as well as promote infl ammation.…”