Metabolism of Alkanes by Acinetobacter

Asperger, O.; Kleber, Hans‐Peter

doi:10.1007/978-1-4899-3553-3_22

Cited by 29 publications

(14 citation statements)

References 88 publications

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“…This might be due to the preference of Acinetobacter sp. to degrade alkanes over aromatic compounds [34]. The general degradation attributes of the recombinant strain A. baumannii pJES remained unchanged and the preferential alkane degradation of the recombinant strain agrees with the previous observations for the wild-type strain [1].…”

Section: Discussionsupporting

confidence: 89%

Crude oil degradation efficiency of a recombinant Acinetobacter baumannii strain and its survival in crude oil-contaminated soil microcosm

Mishra¹,

Sarma

Lal

2004

FEMS Microbiology Letters

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A hydrocarbon degrading Acinetobacter baumannii S30 strain, isolated from crude oil-contaminated soil, was inserted with the lux gene from the luciferase gene cassette luxCDABE. Soil microcosms were designed to study the degradation efficacy for total petroleum hydrocarbon (TPH) of crude oil by lux-tagged A. baumannii S30 pJES. Bioaugmentation of a TPH-contaminated microcosm with A baumannii S30 pJES showed that TPH levels were reduced from 89.3 to 53.9 g/kg soil in 90 days. Biodegradation of TPH by A baumannii S30 pJES was also monitored in shake flask conditions, which showed a reduction of initial TPH levels by over 50% at the end of 120 h. A lux-PCR-based approach along with the standard dilution plating with selective antibiotics was successfully utilized to monitor the survivability of the lux-tagged strain A. baumannii S30 pJES in soil microcosms and stability of the lux insert in the host strain A. baumannii S30. The selective plating technique indicated the population of A. baumannii S30 pJES to be 6.5+/-0.13 x 10(8) CFU/g at day zero (just after bioaugmentation) and 2.09+/-0.08 x 10(8) CFU/g of soil after 90 days of incubation. lux-PCR confirmed the stability of the insert in all the randomly selected colonies of A. baumannii strains from the antibiotic plates. The lux insert was stable after 50 generations in Luria Bertini broth and storage at -70 degrees C as glycerol stocks for over a year. These results revealed that the lux insert was stable and lux-tagged A. baumannii S30 strain could survive in a TPH-contaminated soil microcosm and could degrade TPH in the soil microcosm conditions. It can be used as an effective marker to monitor the survival of augmented strains at a bioremediation site.

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Section: Discussionsupporting

confidence: 89%

Crude oil degradation efficiency of a recombinant Acinetobacter baumannii strain and its survival in crude oil-contaminated soil microcosm

Mishra¹,

Sarma

Lal

2004

FEMS Microbiology Letters

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“…Future experiments with truncated forms of CYP153 as well as elucidation of its structure should shed light on these questions. Independently of this, the features deduced from the AA sequence including the unusual length correspond to the peculiar properties of isolated CYP153 mentioned above (40).…”

Section: Figmentioning

confidence: 91%

Molecular Characterization of the 56-kDa CYP153 from Acinetobacter sp. EB104

Maier

Förster

Asperger

et al. 2001

Biochemical and Biophysical Research Communications

124

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“…The primary attack on the chemically inert alkane is achieved by hydroxylation involving either a cytochrome P450 as shown for A , calcoacetict/s EB104 or a rubredoxin-dependent hydroxylase (Asperger & Kleber, 1991 ;Leahy & Colwell, 1990 ; Claus e t a/., 1980). P450-dependent alkane hydroxylases also occur in Candida tropicalis (Sanglard & Loper, 1989), for example.…”

Section: Introductionmentioning

confidence: 99%

Two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in Acinetobacter calcoaceticus ADP1

1995

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Mutants of Acinetobacter calcoaceticus ADP1 unable to grow on dodecane, but retaining the ability to grow on lauric acid were isolated after ethylmethanesulphonate (EMS) treatment. This growth deficiency was complemented by a clone from a gene library constructed from chromosomal DNA of the wild-type strain. The complementing DNA mapped in a gene encoding a polypeptide with homology to rubredoxins. The deduced putative rubredoxin amino acid sequence is more similar to related proteins from Gram-positive bacteria than to the Pseudomonas oleovorans rubredoxin involved in alkane oxidation. An adjacent gene encodes a protein with similarity to rubredoxin reductase from Pseudomonas oleovorans and related NAD(P)-dependent reductases. Disruption of the rubredoxin-encoding gene by insertion of a KmR/lacZ cassette rendered the resulting strain unable to grow on dodecane or hexadecane. This demonstrates that these genes are necessary for alkane degradation. Transcriptional fusion of lacZ to the rubredoxin-encoding gene led to low level constitutive beta-galactosidase expression, whereas the fusion oriented in the opposite direction was not expressed.

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Metabolism of Alkanes by Acinetobacter

Cited by 29 publications

References 88 publications

Crude oil degradation efficiency of a recombinant Acinetobacter baumannii strain and its survival in crude oil-contaminated soil microcosm

Crude oil degradation efficiency of a recombinant Acinetobacter baumannii strain and its survival in crude oil-contaminated soil microcosm

Molecular Characterization of the 56-kDa CYP153 from Acinetobacter sp. EB104

Two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in Acinetobacter calcoaceticus ADP1

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