Molecular Characterization of the 56-kDa CYP153 from Acinetobacter sp. EB104

Maier, Τ.; Förster, Hans-Heinrich; Asperger, O.; Hahn, Ulrich

doi:10.1006/bbrc.2001.5449

Cited by 124 publications

(103 citation statements)

References 35 publications

(18 reference statements)

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“…EB104 (Fig. 3b), which catalyzes the terminal oxidation of alkanes 22 . Thus, the three P450 systems are likely to account for an important part of the expanded hydrocarbon degradation capacity of A. borkumensis SK2.…”

Section: Genomic Islands and Mobile Genetic Elementsmentioning

confidence: 99%

Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis

et al. 2006

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Alcanivorax borkumensis is a cosmopolitan marine bacterium that uses oil hydrocarbons as its exclusive source of carbon and energy. Although barely detectable in unpolluted environments, A. borkumensis becomes the dominant microbe in oil-polluted waters. A. borkumensis SK2 has a streamlined genome with a paucity of mobile genetic elements and energy generation–related genes, but with a plethora of genes accounting for its wide hydrocarbon substrate range and efficient oil-degradation capabilities. The genome further specifies systems for scavenging of nutrients, particularly organic and inorganic nitrogen and oligo-elements, biofilm formation at the oil-water interface, biosurfactant production and niche-specific stress responses. The unique combination of these features provides A. borkumensis SK2 with a competitive edge in oil-polluted environments. This genome sequence provides the basis for the future design of strategies to mitigate the ecological damage caused by oil spills. Supplementary information The online version of this article (doi:10.1038/nbt1232) contains supplementary material, which is available to authorized users.

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Section: Genomic Islands and Mobile Genetic Elementsmentioning

confidence: 99%

Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis

et al. 2006

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“…EB104 [205]. In contrast to the eukaryotic membrane-bound P450 systems, the CYP153 appeared to occur as a soluble protein [206] like other bacterial P450s involved in other Chapter 1 -General Introduction 33 metabolic pathways.…”

Section: Cytochrome P450 Alkane Hydroxylasesmentioning

confidence: 99%

“…In contrast to the eukaryotic membrane-bound P450 systems, the CYP153 appeared to occur as a soluble protein [206] like other bacterial P450s involved in other Chapter 1 -General Introduction 33 metabolic pathways. This purified P450 exhibited n-alkane-oxidizing activity in the presence of ferredoxin and ferredoxin reductase isolated from the same bacterium [207] and it seemed to be induced by a variety of hydrocarbons [205,207]. Subsequently, highly degenerate primers were designed based on conserved sequence motifs in CYP153A1 and two CYP153…”

Section: Cytochrome P450 Alkane Hydroxylasesmentioning

confidence: 99%

“…Different degenerate primer pairs have been described in literature to be able to amplify conserved regions of different classes of P450 cytochromes. Indeed, the P450 cytochromes are a large and diverse group of enzyme that catalyzes the oxidation of a big variety of organic compounds [205]. We decided to use the oligonucleotides described in literature that seemed to have the most suitable characteristics for our purpose that included either targeting a n-alkane degradation system or being specific for Rhodococcus genus peculiar codon usage.…”

Section: Appendix -Detection Of Other Monooxygenases In Rhodococcus Smentioning

confidence: 99%

“…By contrast, only limited evidence showed the presence of P450 cytochromes involved in n-alkane metabolism in bacteria [30]. In 2001, a cytochrome P450 belonging to a new family (CYP153A1) was reported to be involved in n-hexane oxidation in Acinetobacter sp.EB104 [205]. In contrast to the eukaryotic membrane-bound P450 systems, the CYP153 appeared to occur as a soluble protein [206] like other bacterial P450s involved in other Chapter 1 -General Introduction 33 metabolic pathways.…”

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Monooxygenases involved in the n-alkanes metabolism by Rhodococcus sp. BCP1: molecular characterization and expression of alkB gene

Cappelletti

Fedi

Honda

et al. 2010

Journal of Biotechnology

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Discussion 107 Summary 113Chapter 5 -Analysis of the alkB gene expression Summary 157Chapter 6 -Proteomic analysis of n-alkanes growth of Rhodococcus sp. BCP1 Chapter 1 -General Introduction PrefaceThe quality of life on Earth is tightly related with the overall quality of the environment [1]. During the last century it was commonly believed that the abundance of land and resources were unlimited and it was rarely acknowledged that the production, use, and disposal of hazardous substances had environmental and health effects [1][2][3]. Owing to this, human activity has deliberately or inadvertently released into waters and soil a variety of toxic compounds as refrigerants, paints, solvents, herbicides and pesticides that can cause considerable environmental pollution and human health problems as a result of their persistence, toxicity, and transformation into hazardous metabolites [4,5]. Very soon, however, it became clear how the carelessness and negligence in handling the industrial wastes and gas emissions had caused dramatic effect on the nature and on human health [1].Nowadays, the concern about the global warming and the climate changing induced the government regulatory agencies to establish procedures for assessing the environmental impact and health hazards of chemicals and for controlling/limiting their utilization.International efforts have been applied to remedy many contaminated sites all over the world, either as a response to the risk of adverse health or environmental effects caused by contamination or to enable the site to be redeveloped for use [5]. In response to public and government concern and because of the intriguing research problems presented, environmental scientists, biologists and chemists have been giving increased attention to identifying and determining the behavior and fate of organic compounds in natural ecosystems [5].Bioremediation procedures offer an economical and effective possibility to degrade or render innocuous toxic pollutants using natural biological activity [1]. By definition, bioremediation implies the use of living organisms, primarily microorganisms, to degrade the Since contaminant compounds are transformed by living organisms through reactions that take place as a part of their metabolic processes, environmental biotechnology focuses on the development of techniques to optimize environmental parameters to allow these metabolic pathways to proceed faster and, therefore, to improve the bio-degradation [1]. As a result, the removal of the contaminated soil can be necessary to let the biodegradation proceed in controlled sites (such as bioreactors). These ex situ approaches provide optimal conditions out one single step of the entire bio-degradation process. Hydrocarbons in the environmentHydrocarbons are energy-rich organic compounds consisting of carbon and hydrogen atoms and they can be saturated (only single C-H bounds) or unsaturated (one ore more double or triple C-H bounds). Alkanes are saturated hydrocarbons with the general formula C n H 2n+2 ; they can b...

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