Metabolism and Disposition of Isoflavone Conjugated Metabolites in Humans after Ingestion of Kinako

Hosoda, Kaori; Furuta, Takahisa; Ishii, Kazuo

doi:10.1124/dmd.111.038281

Cited by 52 publications

(49 citation statements)

References 30 publications

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“…The similarities in T max values and variation in plasma concentrations observed between Te-7G-4 S and Te-7G were observed between Te-7S and tectorigenin. Similar pharmacokinetic data have been previously described for plasma concentrations of daidzein, genistein, and their conjugated metabolites in humans after Kinako administration [27]. These results suggest the differential biliary excretion and reabsorption of conjugated tectoridin metabolites and tectorigenin [35], and further study to compare biliary excretion and urinary excretion in bile duct-cannulated rats and control animals is needed to confirm the enterohepatic recirculation of the metabolites.…”

Section: Discussionsupporting

confidence: 84%

See 1 more Smart Citation

Pharmacokinetics of conjugated metabolites in rat plasma after oral administration of tectoridin

Qu¹,

Gao²,

Sun³

et al. 2012

Journal of Chromatography B

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Section: Discussionsupporting

confidence: 84%

“…The same pathways were also demonstrated in the metabolism of daizein, genistein, and quercetin glucosides after their oral intake in humans [25][26][27]. Thus, tectoridin undergoes extensive conjugation to produce its glucuronide, sulfate, and glucuronide-sulfate bis-conjugate in the body after hydrolysis by intestinal flora.…”

Section: Discussionsupporting

confidence: 52%

Pharmacokinetics of conjugated metabolites in rat plasma after oral administration of tectoridin

Qu¹,

Gao²,

Sun³

et al. 2012

Journal of Chromatography B

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“…However, in pharmacokinetic studies, IF concentrations in urine are usually very high. Therefore, urine samples have to be diluted [24][25][26]. We investigated the matrix effect in the same blank urine samples after diluting these 10 times with water.…”

Section: Recovery and Matrix Effectmentioning

confidence: 99%

Quantification of soy isoflavones and their conjugative metabolites in plasma and urine: an automated and validated UHPLC-MS/MS method for use in large-scale studies

et al. 2014

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The biotransformation of isoflavones by gut microbiota and by drug metabolizing enzymes plays a crucial role in the understanding of their potential health-promoting effects. The purpose of our work was to develop a simultaneous, sensitive, and robust automated ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method to quantify the soy isoflavones daidzein and genistein, their conjugative metabolites, as well as their major microbial degradation products in order to provide a method for use in large clinical trials or animal studies. An automated, 96-well solid-phase extraction method was used to extract the isoflavone analytes from plasma and urine. Separation of genistein, daidzein, and 19 of its metabolites, including five glucuronides, seven sulfates, and two sulfoglucuronides, as well as five microbial metabolites, was achieved in less than 25 min using a sub-2 μm particle column and a gradient elution with acetonitrile/methanol/water as mobile phases. Analysis was performed under negative ionization electrospray MS via the multiple reaction monitoring (MRM). Validation was performed according to the analytical method validation guidelines of Food and Drug Administration (FDA) and International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) consisting of selectivity, accuracy, precision, linearity, limit of detection, recovery, matrix effect, and robustness. All validated parameters essentially matched the FDA and ICH requirements. The application of this method to a pharmacokinetic study in postmenopausal women showed that isoflavones are extensively metabolized in vivo. A robust automated analytical approach was developed, which allows the handling of large sample sizes but nevertheless provides detailed information on the isoflavone metabolite profile leading to a better understanding and interpretation of clinical and animal studies.

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“…Recently, several studies have reported the metabolism of puerarin and daidzin [3,[15][16][17][18]. Luo et al [15] have reported that glucuronidation is the major metabolic pathway for puerarin when rats were given puerarin intravenously.…”

Section: Introductionmentioning

confidence: 98%

Pharmacokinetic comparisons of puerarin, daidzin and the glucuronide metabolite of puerarin after administration of total flavonoid from Gegen alone and total flavonoid from Gegen combined with total saponin from Sanqi in rats under different physiological states

Liu

Zhao

Gao

et al. 2013

Journal of Chromatography B

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Metabolism and Disposition of Isoflavone Conjugated Metabolites in Humans after Ingestion of Kinako

Cited by 52 publications

References 30 publications

Pharmacokinetics of conjugated metabolites in rat plasma after oral administration of tectoridin

Pharmacokinetics of conjugated metabolites in rat plasma after oral administration of tectoridin

Quantification of soy isoflavones and their conjugative metabolites in plasma and urine: an automated and validated UHPLC-MS/MS method for use in large-scale studies

Pharmacokinetic comparisons of puerarin, daidzin and the glucuronide metabolite of puerarin after administration of total flavonoid from Gegen alone and total flavonoid from Gegen combined with total saponin from Sanqi in rats under different physiological states

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