Pharmacokinetics of conjugated metabolites in rat plasma after oral administration of tectoridin

Qu, Jialin; Gao, Jie; Sun, Jiahong; Zhang, Lin; Makino, Toshiaki; Yuan, Dan

doi:10.1016/j.jchromb.2012.06.017

Cited by 19 publications

(11 citation statements)

References 32 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, calycosin was not detected in rat plasma, probably resulting from its strong phase II metabolism in liver. It was often found that high levels of phase II metabolites circulated in blood with trace amounts of aglycone in the pharmacokinetic profiles of isoflavones (Bai, Qu, Lu, Kano, & Yuan, ; Hosoda, Furuta, & Ishii, ; Qu et al, ; Tian et al, ; Wang, Gong, et al, ; Yuan et al, ; Zhang, Qi, Xu, Kano, & Yuan, ). Considerably different pharmacokinetics of CAG and calycosin have been published in the literature after rats were treated with TCM consisting of Huangqi (Liu et al, ; Liu et al, ; Shaw et al, ; Shi et al, ; Tao, Huang, Yang, Li, & Cai, ; Zeng et al, ; Zhang, Yang, Lin, & Hu, ).…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination of major components of Huangqi–Honghua extract in rat plasma using LC–MS/MS and application to a pharmacokinetic study

Wang

Qiao

et al. 2019

Biomedical Chromatography

View full text Add to dashboard Cite

A sensitive and reliable LC-MS/MS method was developed and validated for simultaneous quantification of the major components of Huangqi-Honghua extact in rat plasma, including hydroxysafflor yellow A (HSYA), astragaloside IV (ASIV), calycosin-calycosin-3′-O-sulfate (C-3′-S). After extraction by protein precipitation with acetonitrile and methanol from plasma, the analytes were separated on a Hypersil BDS C 18 column by gradient elution with acetonitrile and 5 mM ammonium acetate. The detection was carried out on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source switched between negative and positive modes.HSYA was monitored in negative ionization mode from 0 to 4.9 min, and ASIV, CAG, calycosin, C-3′-G and C-3′-S were determined in positive ionization mode from 4.9 to 10 min. The lower limits of quantification of the analytes were 6.25 ng/mL for HSYA, 0.781 ng/mL for CAG and 1.56 ng/mL for ASIV and calycosin. The intra-and inter-assay precision (RSD) values were within 13.43%, and accuracy (RE) ranged from −8.75 to 9.92%. The validated method was then applied to the pharmacokinetic study of HSYA, ASIV, CAG, calycosin, C-3′-G and C-3′-S in rat after an oral administration of Huangqi-Honghua extract.

show abstract

Section: Resultsmentioning

confidence: 99%

Simultaneous determination of major components of Huangqi–Honghua extract in rat plasma using LC–MS/MS and application to a pharmacokinetic study

Wang

Qiao

et al. 2019

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…To calculate the administered dose, the contents of three major ingredients were quantitatively determined by reported HPLC-UV with external standard method [15]. Results indicated that the contents of tectorigenin-7-O-β-D-xylosylglucopyranoside (10), tectoridin (12) and tectorigenin (25) in the extract were 112.5, 96.64 and 19.81 mg/g, respectively.…”

Section: Preparation Of Pueraria Flos Extractsmentioning

confidence: 99%

Pueraria Flos Alleviates Alcoholic Liver Injury Via Regulation of PPARα and MAOA: Deciphering the Effective Forms and Potential Mechanism Based on a Bioinformatics-Integrated Metabolic Profile Strategy and Experimental Validation

Chen

Wei

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Pueraria Flos, a representative medicinal and edible antidote for alcoholism, has rich clinical experience and remarkable curative effect in the treatment of alcoholic liver disease (ALD). However, its effective forms and hepatoprotective mechanism are remained unknown. Methods: A strategy based on UPLC-QTOF-MS combined with mass defect filtering technique was established for comprehensively identifying prototypes and metabolites absorbed and excreted into rat plasma, urine, bile and feces after oral administration. Then, the absorbed constituents with a relative high level were subjected to the network pharmacology, functional enrichment analysis and molecular docking to clarify the potential mechanism in the treatment of ALD. Furthermore, the therapeutic effect of PF on ALD and predicted mechanisms was further evaluated using a rat model of alcohol-induced liver injury and Western blot analysis. Results: 25 absorbed prototype constituents and 82 metabolites were identified or tentatively characterized with glucuronidation, sulfation, methylation, hydroxylation and reduction as their major metabolic pathways. The constructed absorbed constituent-target-pathway-disease network and docking analysis revealed that 4 metabolic components Te-7XG, genistein-7G-4'S, tectoridin-4'S and Te-7XG-4'S, 2 targets MAOA and PPARA, and 6 pathways related to lipid regulation and amino acid metabolism may play crucial roles in the PF mediated protection against ALD. An in vivo validation in rat further demonstrated that PF alleviated liver injury via activating and suppressing the PPARA and MAOA expression, respectively. Conclusions: The present results not only increase the understanding on the effective form and molecular mechanism of PF mediated protection against ALD, but also promote better application of PF as supplement food and herbal medicine for the treatment of ALD.

show abstract

“…The extensive Phase II metabolism plays an important role in the pharmacokinetics of tectoridin and tectorigenin in vivo . 36 …”

Section: Hawthorn (山楂 Shān Zhā)mentioning

confidence: 99%

Pharmacokinetic profile of phytoconstituent(s) isolated from medicinal plants—A comprehensive review

Mehta

Shah

Lohidasan

et al. 2015

Journal of Traditional and Complementary Medicine

View full text Add to dashboard Cite

Herbal medicine, the backbone of traditional medicine, has played an important role in human health and welfare for a long period. Traditional therapeutic approaches of regional significance are found in Africa, South and Central America, China, India, Tibet, Indonesia, and the Pacific Islands. The considerable scientific significance and commercial potential of traditional medicines have resulted in increased international attention and global market demands for herbal medicines, especially Chinese herbal medicines. Herbal medicines currently are the primary form of health care for the poor in the developing countries, and also are widely used as a supplement or substitute for conventional drugs in developed countries. These traditional medicines have a pivotal role in the treatment of various ailments and more than 50% of drugs used in Western pharmacopoeia are isolated from herbs or derived from modifications of chemicals found in plants. Herbal medicines usually contain a complex mixture of various bioactive molecules, which make its standardization complicated, and there is little information about all compounds responsible for pharmacological activity. Several research papers have been published that claim pharmacological activity of herbal medicines but few are discussing the role of the exact phytoconstituent. Understanding the pharmacokinetic profile of such phytoconstituents is essential. Although there are research papers that deal with pharmacokinetic properties of phytoconstituents, there are a number of phytoconstituents yet to be explored for their kinetic properties. This article reviews the pharmacokinetic profile of 50 different therapeutically effective traditional medicinal plants from the year 2003 onward.

show abstract

Pharmacokinetics of conjugated metabolites in rat plasma after oral administration of tectoridin

Cited by 19 publications

References 32 publications

Simultaneous determination of major components of Huangqi–Honghua extract in rat plasma using LC–MS/MS and application to a pharmacokinetic study

Simultaneous determination of major components of Huangqi–Honghua extract in rat plasma using LC–MS/MS and application to a pharmacokinetic study

Pueraria Flos Alleviates Alcoholic Liver Injury Via Regulation of PPARα and MAOA: Deciphering the Effective Forms and Potential Mechanism Based on a Bioinformatics-Integrated Metabolic Profile Strategy and Experimental Validation

Pharmacokinetic profile of phytoconstituent(s) isolated from medicinal plants—A comprehensive review

Contact Info

Product

Resources

About