Metabolic types of muscle in the sheep: I. Myosin ATPase, gloycolytic, and mitochondrial enzyme activities

Briand, M.; Talmant, André; Briand, Yves; Monin, Gabriel; Durand, Roger

doi:10.1007/bf00422122

Cited by 111 publications

(45 citation statements)

References 27 publications

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“…Similar observation can be made between the SP and the other muscles in the R, RPF and RAL animals. In lambs, the SP muscle is considered as a slow red muscle (high oxidative capacity), whereas LD and ST muscles are fast red and fast white muscles (high glycolytic capacity), respectively (Briand et al, 1981a and1981b). This was in agreement with the reported findings in the literature where carnosine and anserine concentrations have been demonstrated to increase in muscles with intense glycolytic activity in many species (Sewell et al, 1992;Aristoy and Toldra, 1998;Cornet and Bousset, 1999).…”

Section: Resultssupporting

confidence: 84%

Food restriction and refeeding in lambs influence muscle antioxidant status

Savary-Auzeloux

Durand

Gruffat

et al. 2008

Animal

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Compensatory growth, a frequent phenomenon observed in ruminants due to seasonal variation in food availability, affects protein metabolism including protein oxidation. These oxidation processes may have an impact on animal health as well as on meat protein degradation during post mortem aging (ie meat maturation). Sixteen male lambs were randomly divided into four groups. One group was fed ad libitum (C) and one group was food-restricted to 60% of the intake of the C group (R). The last two groups were restricted similarly to the R group and refed either ad libitum (RAL) or similarly to the C group (pair-feeding) (RPF). Muscles samples were taken immediately after slaughter. The present study showed that the restriction/refeeding pattern had no effect on protein oxidation in the muscles studied (longissimus dorsi (LD), semitendinosus (ST) and supraspinatus (SP)). However, total antioxidant capacity decreased after food restriction (251%, 243%, P , 0.01 for ST and LD muscles, respectively) and re-increased only after ad libitum refeeding. This alteration in the total antioxidant status can partially be explained by the similar pattern of change observed in the glutathione concentration of the muscles (225%, P , 0.05 for ST muscle and NS for the other muscles). However, none of the concentrations of other water-soluble antioxidants studied (carnosine, anserine, glutathione peroxidase and superoxide dismutase) were altered during compensatory growth. This study showed that an inappropriate feeding level following a nutritional stress induced alterations in the total antioxidant status (particularly that of glutathione), which may have consequences on animal health. Other consequences of a decrease of the animal antioxidant status in vivo could be an alteration of the protein oxidation processes during meat maturation.Keywords: antioxidant status, compensatory growth, muscle, sheep IntroductionIn the muscle post mortem, some proteins are oxidized and this process has an impact on meat tenderization (Maltin et al., 2003). Increased proteases oxidation reduces the functional properties of these proteases and decreases their efficiency in the meat protein maturation process (Rowe et al., 2004a and2004b). Consequently, it can be hypothesized that some in vivo oxidation processes in the muscles may have an impact on the enzymes activities and protein structure both in vivo and post mortem (Stadtman and Levine, 2000). To counteract these oxidation processes, many reactive oxygen species (ROS) scavengers exist within the cells (Fang et al., 2002). Among these ROS, the watersoluble antioxidants (some antioxidant enzymes, peptides or vitamins soluble in water) are of major significance to counteract protein oxidation and carbonyl formation (Hipkiss et al., 1998 and.Among the various stress affecting ruminants, the seasonal variations in forage availability during extensive rearing affect protein metabolism with as a result, for example, compensatory growth (Hoch et al., 2003). The alteration of the growth pattern during com...

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Section: Resultssupporting

confidence: 84%

Food restriction and refeeding in lambs influence muscle antioxidant status

Savary-Auzeloux

Durand

Gruffat

et al. 2008

Animal

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“…A small portion of loin muscle was collected adjacent to the 12 th rib as soon as possible (a maximum of 5 hours) post mortem to measure ICDH enzyme activity. A 1 g portion of muscle was snap-frozen in liquid nitrogen and stored at -80 °C until ICDH activity could be measured according to the method of Briand (1981).…”

Section: Carcass and Muscle Sampling Detailsmentioning

confidence: 99%

Production factors influence fresh lamb longissimus colour more than muscle traits such as myoglobin concentration and pH

et al. 2016

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M.longissimus colour was measured from 8165 lambs at 24 hours post-mortem using a chromameter. The impact of production factors (site and year of production, slaughter group, sex, age and breed type) and muscle traits (hot carcass weight, pH 24 , isocitrate dehydrogenase (ICDH) activity, myoglobin, iron and zinc concentrations) on meat lightness (L*), redness (a*), yellowness (b*), hue and chroma were analysed. Greater differences in meat colour were seen between different slaughter groups and sites of production than across the range of any muscle traits. Of the muscle traits analysed, changes in pH 24 had the greatest effect on meat a* (2.5 units), while myoglobin had the greatest effect on meat L* (2.9 units). The 3.1 L* unit darkening of meat with increasing lamb age (from 140 to 400 days) was accounted for by increased myoglobin concentration. These results suggest that production factors are having substantial effects on lamb colour independent of known influencing muscle traits such as myoglobin concentration and pH.

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“…This will result in a metabolic shift towards a more glycolytic metabolism in the muscle. Higher proportions of type IIX muscle fibres and glycolytic metabolism are also associated with a greater expression of glycogen phosphorylase (Briand et al, 1981;Saltin and Gollnick, 1983;Greenwood et al, 2006;Gardner et al, 2006a), the key enzyme in glycogen breakdown. A metabolic shift of these muscle types are likely to make the animal more susceptible to adrenaline-induced glycogen depletion during stress (Gardner et al, 1999), reflected by the elevation of plasma lactate concentration.…”

Section: Introductionmentioning

confidence: 99%

Progeny of high muscling sires have reduced muscle response to adrenaline in sheep

Martin

McGilchrist

Thompson

et al. 2011

Animal

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This study investigated the impact of variation in Australian sheep breeding values (ASBVs) for yearling eye muscle depth (YEMD) within Merino and Poll Dorset sires on intermediary metabolism of progeny. Specifically, the change in the blood concentrations of lactate, non-esterified fatty acids (NEFA) and glucose in response to administration of an exogenous dose of adrenaline was studied. The experiment used 20 Merino and Merino cross Poll Dorset mixed sex sheep. The sires were selected across a range of YEMD ASBVs. The sheep were fitted with indwelling jugular catheters and administered seven levels of adrenaline over a period of 4 days at 4 months of age (0.1, 0.2, 0.4, 0.6, 0.9, 1.2 and 1.6 mg/kg liveweight (LW)) and 16 months of age (0.1, 0.2, 0.6, 1.2, 1.8, 2.4 and 3.0 mg/kg LW). A total of 16 blood samples were collected between 230 min and 130 min relative to administration of the adrenaline challenge and were later measured for the plasma concentrations of lactate, NEFA and glucose. These data were then used to calculate the time to maximum substrate concentration, the maximum concentration and the area under curve (AUC) between 0 and 10 min, thus reflecting the substrate's response to exogenous adrenaline. Selection for muscling led to decreased muscle response due to adrenaline, as indicated by lower maximum concentrations and AUC for lactate. The muscles' response to adrenaline was more prominent at 16 months of age than at 4 months of age. Thus, animals selected for increased muscling have lower levels of glycogenolysis in situations where endogenous adrenaline levels are increased like pre-slaughter. This may minimise the risk of poor meat quality in these animals, as they will express higher muscle concentrations of glycogen at slaughter. Adipose tissue was more sensitive to adrenaline in young lambs from high YEMD sires. This shows that high muscled growing lambs utilise their adipose tissue deposits in times of stress to produce energy. This may explain the phenotypic leanness of these animals. Blood glucose levels that are indicative of liver response to adrenaline decreased with selection for muscling. This response may indicate a potential limiting of glucose that is available within animals selected for muscling, leanness and growth for brain function.

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Metabolic types of muscle in the sheep: I. Myosin ATPase, gloycolytic, and mitochondrial enzyme activities

Cited by 111 publications

References 27 publications

Food restriction and refeeding in lambs influence muscle antioxidant status

Food restriction and refeeding in lambs influence muscle antioxidant status

Production factors influence fresh lamb longissimus colour more than muscle traits such as myoglobin concentration and pH

Progeny of high muscling sires have reduced muscle response to adrenaline in sheep

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