Metabolic requirements associated with GSH synthesis during in vitro maturation of cattle oocytes

Furnus, Cecilia Cristina; Matos, D.G. de; Picco, Sebastián Julio; García, Pilar Peral; Inda, Ana María; Mattioli, Guillermo Alberto; Errecalde, Ana Lı́a

doi:10.1016/j.anireprosci.2007.12.003

Cited by 69 publications

(52 citation statements)

References 46 publications

(55 reference statements)

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“…GSH content at the end of maturation has been identified as a reliable indicator of oocyte viability (Abeydeera et al 1999). GSH synthesis is based on the presence of glycine, glutamine, and cysteine, of which cysteine is the rate-limiting step (Furnus et al 2008). Therefore, supplementation of cysteine during maturation resulted in improved development to the morula and blastocyst stages (Ali et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development

Vandaele¹,

Thys²,

Bijttebier³

et al. 2010

Reproduction

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Recent studies have shown that short-term exposure of oocytes to a stressor such as hydrostatic pressure or osmotic stress might induce stress tolerance in embryos. The aim of the present study was to investigate the consequences of short-term hydrogen peroxide (H 2 O 2 ) exposure to bovine in vitro matured cumulus-oocyte complexes (COCs) on subsequent preimplantation embryo development and apoptosis. In the first experiment, mature COCs were incubated in H 2 O 2 at concentrations ranging between 0.01 and 100 mmol/l, and subsequently fertilized and cultured. Oocyte incubation with 50-100 mmol/l of H 2 O 2 resulted in a significantly higher blastocyst yield (47.3%) in comparison with control medium (31.8%), while apoptotic cell ratio was inversely related with H 2 O 2 concentration. In the second experiment, we showed that the stress tolerance after H 2 O 2 exposure was not mediated by increased glutathione content in treated oocytes nor by enhanced fertilization or penetration. Further research should concentrate on the potential role of players that have been associated with stress tolerance in somatic cell lines.

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Section: Discussionmentioning

confidence: 99%

Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development

Vandaele¹,

Thys²,

Bijttebier³

et al. 2010

Reproduction

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“…Cytoplasmic glutathione (L-γ-glutamyl-L-cysteinyl-glycine; GSH) is the major non protein sulphydryl compound in mammalian gametes; it serves as a reservoir for cysteine and plays an important role in protecting mammalian gametes from apoptosis induced by reactive oxygen species [19][20][21][22]. On the other hand, in several reports, GSH has been introduced as an important indicator of cytoplasmic maturation [20,23,24].…”

Section: Introductionmentioning

confidence: 99%

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Abazari-Kia

Mohammadi‐Sangcheshmeh

Dehghani-Mohammadabadi

et al. 2013

J Assist Reprod Genet

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Purpose To associate glucose-6-phosphate dehydrogenase (G6PDH) activity in goat oocytes with intracellular glutathione (GSH) content, meiotic competence, developmental potential, and relative abundance of Bax and Bcl-2 genes transcripts. Methods Goat oocytes were exposed to brilliant cresyl blue (BCB) staining test and categorized into BCB + (bluecytoplasm), and BCB − (colorless-cytoplasm) groups. A group of oocytes were not exposed to BCB test and was considered as a control group. After maturation in vitro, a group of oocytes were used for determination of nuclear status and intracellular GSH content while another group was subjected to parthenogenetic activation followed by in vitro embryo culture.Results We found that BCB + oocytes not only yielded higher rate of maturation, but also showed an increased level of intracellular GSH content than BCB − and control oocytes.Furthermore, BCB + oocytes produced more blastocysts than BCB − and control oocytes. Our data revealed that the expression of anti-apoptotic (Bcl-2) and pro-apoptotic (Bax) genes were interacted with G6PDH-activity in mature oocyte, their surrounding cumulus cells, and blastocyst-stage embryos.Conclusions The results of this study demonstrate that selection of goat oocytes based on G6PDH-activity through the BCB test improves their developmental competence, increases intracellular GSH content, and affects the expression of the apoptosis-related genes.

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“…No entanto, a literatura descreve que a presença de antioxidante é essencial para a maturação de oócitos, particularmente para a maturação citoplasmática, necessária para o desenvolvimento embrionária (Furnus et al, 2008;Silva, et al, 2011). A maturação citoplasmática está diretamente relacionada com a taxa de desenvolvimento embrionário (Rizos et al, 2002) (Rizos et al, 2002;Sananmuang et al, 2011).…”

Section: Discussionunclassified

“…Essa similaridade pode ser pela disponibilidade de aminoácidos precursores de GSH no meio de MIV e pode atuar estimulando a síntese de GSH. Segundo Furnus et al (2008), a concentração de GSH aumenta em oócitos bovinos durante o processo da MIV decorrente dos aminoácidos disponíveis nos meios de maturação. Estudos mais aprofundados serão necessários para entender melhor as relações entre quercetina e a PIV de embriões e os níveis de GSH em oócitos bovinos.…”

Section: Discussionunclassified

Maturação in vitro de oócitos bovinos em meios suplementados com quercetina e seu efeito sobre o desenvolvimento embrionário

Guemra

Monzani

Santos³

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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RESUMOA quercetina é um flavonoide, amplamente encontrada em frutas, vegetais, grãos, flores, com elevada concentração no vinho tinto, e tem sido caracterizada funcionalmente pela atividade antioxidante. Para avaliação da maturação nuclear e do desenvolvimento embrionário bovino, os oócitos foram maturados por 22h na presença de quercetina (0,4, 2, 10 e 50M), cisteamina (100M) e na ausência dos antioxidantes. Os oócitos maturados foram corados com Hoechst para avaliação da maturação in vitro. Para avaliação do desenvolvimento embrionário, os oócitos foram fertilizados e cultivados in vitro, as taxas de desenvolvimento embrionário foram determinadas no sétimo dia de cultivo e o percentual de eclosão e o número de células dos embriões no oitavo dia. Os níveis de glutationa (GSH) dos oócitos foram mensurados por emissão de fluorescência com CMF 2 HC. A porcentagem de maturação nuclear (±89%) não diferiu entre os grupos. O desenvolvimento embrionário variou entre os tratamentos, o percentual de blastocisto foi superior (P<0,05) nos grupos tratados com 0,4, 2, 10 e 50M de quercetina (56,9, 59,5, 53,6 e 49,6%, respectivamente) e com 100M de cisteamina (50,4%) em relação ao grupo controle (42,3%). Na comparação entre os dois antioxidantes, a quercetina (0,4 e 2µM) foi superior na produção de embriões (56,9 e 59,5%, respectivamente) em comparação com cisteamina (50,4%). As taxas de embriões eclodidos foram similares (P>0,05) entre os grupos (±63,0%). O número médio de células dos embriões também foi similar entre os grupos (±233). Os níveis intracelulares de GSH foram superiores nos oócitos maturados com cisteamina, mas similares entre os oócitos tratados com quercetina e o controle. A suplementação da maturação in vitro com antioxidantes melhora as taxas de blastocistos. A quercetina foi superior à cisteamina, que, por sua vez, foi superior ao controle. Mas os níveis de GSH foram superiores somente nos oócitos tratados com cisteamina. Palavra-chave: antioxidante, bovino, embrião, quercetina e oócito ABSTRACTQuercetin is a flavonoid widely found in fruit, vegetables, grains and flowers, with a high concentration in red wine, and has been functionally characterized by its antioxidant activity. For assessment of nuclear maturation and bovine embryo, oocytes were matured for 22h in the presence of quercetin (0.4, 2, 10 and 50µM), cysteamine (100µM)

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Metabolic requirements associated with GSH synthesis during in vitro maturation of cattle oocytes

Cited by 69 publications

References 46 publications

Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development

Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Maturação in vitro de oócitos bovinos em meios suplementados com quercetina e seu efeito sobre o desenvolvimento embrionário

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