Metabolic Profiling of Vasorelaxant Extract from <i>Malvaviscus arboreus</i> by LC/QTOF‐MS

Rodrı́guez-Morales, Sergio; Ocampo-Medina, Blanca; Romero-Ceronio, Nancy; Alvarado-Sánchez, Cuauhtémoc; Vilchis‐Reyes, Miguel A.; Roa, Luis F.; Ortíz-Andrade, Rolffy; Hernández-Abreu, Oswaldo

doi:10.1002/cbdv.202000820

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…Malvaviscus arboreous Cav. (MAC) is a perennial deciduous shrub of Malvaceae , natively found from Mexico to Brazil, which is also widely cultivated and naturalized in tropical and subtropical regions including southern China [ 6 ]. It is also called sleeping hibiscus due to its bright red petals that are spirally wrapped, and only the slender stamens and pistils protrude from the petals.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity

et al. 2022

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The ultrasonic-assisted aqueous two-phase extraction (UAATPE) of flavonoid glycosides from Malvaviscus arboreous Cav. flower (MACF) was developed using ethanol/ammonia sulfate systems, followed by the ultrasonic-assisted acid hydrolysis (UAAH) of the top extract with HCl solution. The optimization of UAATPE and UAAH processes was accomplished by single-factor experiments and response surface methodology. As a result, the flavonoid glycosides enriched in the top phase could achieve a maximum yield of 35.9 ± 1.1 mg/g by UAATPE and were completely hydrolyzed by UAAH deglycosylation. The flavonoid glycosides and their hydrolyzates were separated and characterized by high-performance liquid chromatography and ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. Ultrasonic enhancement of the extraction and hydrolysis was explored by comparative study. Furthermore, the in vitro activity of the flavonoid glycosides and the aglycones were comprehensively evaluated by antioxidant activity assays, including ferric-reducing antioxidant power and scavenging DPPH, hydroxyl, and superoxide radicals. All of the IC50 values suggest that the antioxidant activity of flavonoid aglycones was stronger than that of their glucosides and even vitamin C, revealing that the deglycosylated flavonoids from MACF were the more powerful antioxidants. This study provided an effective and eco-friendly strategy for the extraction, separation, and purification of flavonoids from MACF, as well as for the development of the potential flavonoid antioxidants.

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Section: Introductionmentioning

confidence: 99%

“…However, the research was confined to the pharmacological activities of crude extracts of hot water and 95% ethanol. Few further studies have been carried out between the chemical constituents and pharmacological effects of Malvaceae species, especially MAC flower (MACF) [ 6 , 7 , 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%