Metabolic profile associated with distinct behavioral coping strategies of 129Sv and Bl6 mice in repeated motility test

Abstract

We investigated the metabolic outcome of different coping strategies in 129S6/SvEvTac (129Sv) and C57BL/6Ntac (Bl6) strains. Two different batches of male 129Sv and Bl6 mice were used. One batch was not subjected to any behavioral manipulations (home cage control; HCC), whereas the other batch was treated with saline for 11 days and exposed after every treatment to the motor activity measurement (repeated motility tested; RMT). Bl6 RMT mice displayed a robust increase in number of rearings during repeated testing. 129Sv RMT mice experienced significant loss of body weight, but showed enhanced weight gain in HCC batch compared to Bl6. Serum metabolites (acylcarnitines, amino acids, biogenic amines, hexoses, glycerophospholipids and sphingolipids) were determined with AbsoluteIDQ p180 kit. Results of the metabolomic study revealed prominent peculiarities between strains in two different conditions. Comparison of both batches of mice demonstrated that in Bl6 biogenic amines (acetyl-ornithine, alpha-amionadipic acid, carnosine) and lysophosphatidylcholine PC(16:1/0:0) dominated. However in 129Sv acylcarnitine C5 clearly dominated, indicating shift towards short-chain acylcarnitines. Stable strain-specific ratios also emerged for both lines, ratio of glycine/PC ae C38:2 for Bl6 and ratios of C5/C0 as well as PC(16:0/0:0)/PC(16:1/0:0) for 129Sv. The described metabolic changes probably reflect different behavioral coping strategies of 129Sv and Bl6 mice.

Role of Sirt3 in Differential Sex-Related Responses to a High-Fat Diet in Mice

“…However, this has been disproven and without foundation [7]. While numerous papers examined the metabolic profiles of inbred mouse strain 129 [8][9][10], little attention has been paid to the impact of sex on the development of metabolic syndrome caused by HFD.…”

Repeated Administration of D-Amphetamine Induces Distinct Alterations in Behavior and Metabolite Levels in 129Sv and Bl6 Mouse Strains

“…Based on above described data of DBA/2 mice we expect that 129Sv mice display stronger sensitization toward AMPH-induced hyper-locomotion compared to Bl6 strain. Previously, we have found the significant differences in the blood metabolite levels in Bl6 and 129Sv mice (Narvik et al, 2018 ). Thus, we expected to see significant differences in metabolite levels between these two mouse strains in response to repeated AMPH as well, especially in the levels of hexoses and lipid metabolites that are necessary to fuel the strong locomotor response.…”

“…Thus, a preliminary analysis was performed to evaluate the impact of repeated saline administration on metabolic markers. The results indicated that altogether 52 metabolites were significantly different between 129Sv and Bl6 (Narvik et al, 2018 ). The values of 33 items were higher in Bl6 and 19 items were respectively elevated in 129Sv.…”

“…After a thorough literature search we may conclude that we are the first to explore the metabolic profile of these two mouse lines. Furthermore, we have recently shown that after repeated saline administration the 129Sv and Bl6 strains display different metabolic profiles and behavioral coping strategies (Narvik et al, 2018 ). This encouraged us to investigate the metabolic outcomes of repeated AMPH in these two mouse strains.…”

“…Second, 129Sv mice display aberrant adaptation in a stressful environment. Repeated testing of Bl6 mice in the motility cages robustly increased their exploratory activity, whereas in 129Sv no such changes occurred and their activity remained almost at the level of the first testing day (Narvik et al, 2018 ). However, as opposed to Bl6 mice, 129Sv mice started to lose body weight.…”