Repeated Administration of D-Amphetamine Induces Distinct Alterations in Behavior and Metabolite Levels in 129Sv and Bl6 Mouse Strains

Vanaveski, Taavi; Narvik, Jane; Innos, Jürgen; Philips, Mari-Anne; Ottas, Aigar; Plaas, Mario; Haring, Liina; Zilmer, Mihkel; Vasar, Eero

doi:10.3389/fnins.2018.00399

Cited by 12 publications

(7 citation statements)

References 65 publications

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“…A prior study found that methamphetamine exposure reduced the levels of glucose in the plasma of humans [16]. Another study reported an increase in the levels of leucine in mice exposed to repeated administration of d-amphetamine [17]. These findings indicate that exposure to drugs of abuse can induce impairments in protein expressions or functions that regulate the metabolism of glucose and other metabolomics biomarkers.…”

Section: Introductionmentioning

confidence: 85%

Serum Metabolomic Analysis of Male Patients with Cannabis or Amphetamine Use Disorder

et al. 2022

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Studies have demonstrated that chronic consumption of abused drugs induces alterations in several proteins that regulate metabolism. For instance, methamphetamine exposure reduces glucose levels. Fatty and amino acid levels were altered in groups exposed to abused drugs. Therefore, in our study, we investigated the serum metabolomic profile of patients diagnosed with cannabis and/or amphetamine use disorders. Blood was obtained from subjects (control, amphetamine, and cannabis). Detection of serum metabolites was performed using gas chromatography. The ratio peak areas for metabolites were analyzed across the three groups. Both cannabis and amphetamine groups showed higher d-erythrotetrafuranose, octadecanoic acid, hexadecenoic acid, trans-9-octadecanoic acid, lactic acid and methyl thio hydantoin metabolites compared with the control group. Moreover, cannabis patients were found to possess higher glycine, 9,12 octadecanoic acid malonic acid, phosphoric acid and prostaglandin F1a than controls. Our analysis showed that the identified metabolic profile of cannabis or amphetamine use disorder patients was different than control group. Our data indicated that chronic exposure to cannabis or amphetamine dysregulated metabolites in the serum. Future studies are warranted to explore the effects of these abused drugs on the metabolic proteins.

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Section: Introductionmentioning

confidence: 85%

Serum Metabolomic Analysis of Male Patients with Cannabis or Amphetamine Use Disorder

et al. 2022

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“…Particularly in the studied serum samples, a number of amino acids, acyl-carnitines, intermediates of the tricarboxylic acid cycle (TCA), bile and fatty acids have been identified as affected by GHB. The majority of these endogenous compounds relates to energy metabolism in the broadest sense and have also been described in relation to consumption of other DOAs [ 20 , 23 , 35 , 36 , 37 , 38 ]. As such, their suitability as biomarkers for identification of GHB in particular seems questionable, as first the observed fcs were small, particularly considering the highly controlled conditions of the study, and second they seem unlikely to allow differentiation of GHB intake from other DOAs.…”

Section: Discussionmentioning

confidence: 99%

Towards Extending the Detection Window of Gamma-Hydroxybutyric Acid—An Untargeted Metabolomics Study in Serum and Urine Following Controlled Administration in Healthy Men

et al. 2021

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In forensic toxicology, gamma-hydroxybutyrate (GHB) still represents one of the most challenging drugs of abuse in terms of analytical detection and interpretation. Given its rapid elimination, the detection window of GHB in common matrices is short (maximum 12 h in urine). Additionally, the differentiation from naturally occurring endogenous GHB, is challenging. Thus, novel biomarkers to extend the detection window of GHB are urgently needed. The present study aimed at searching new potential biomarkers of GHB use by means of mass spectrometry (MS) metabolomic profiling in serum (up to 16.5 h) and urine samples (up to 8 h after intake) collected during a placebo-controlled crossover study in healthy men. MS data acquired by different analytical methods (reversed phase and hydrophilic interaction liquid chromatography; positive and negative electrospray ionization each) were filtered for significantly changed features applying univariate and mixed-effect model statistics. Complementary to a former study, conjugates of GHB with glycine, glutamate, taurine, carnitine and pentose (ribose) were identified in urine, with particularly GHB-pentose being promising for longer detection. None of the conjugates were detectable in serum. Therein, mainly energy metabolic substrates were identified, which may be useful for more detailed interpretation of underlying pathways but are too unspecific as biomarkers.

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“…Vanaveski et al observed increases of medium-chain acyl carnitines after repeated amphetamine administration in 129Sv mice. From the elevation of the ratio between long chain acyl carnitines to carnitine, the authors also proposed an increased participation of acyl carnitines to account for an additional energy demand [49]. It is also known that immune cells such as leukocytes are enriched in carnitines.…”

Section: Acyl Carnitinesmentioning

confidence: 99%

Comparative Untargeted Metabolomics Analysis of the Psychostimulants 3,4-Methylenedioxy-Methamphetamine (MDMA), Amphetamine, and the Novel Psychoactive Substance Mephedrone after Controlled Drug Administration to Humans

et al. 2020

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Psychoactive stimulants are a popular drug class which are used recreationally. Over the last decade, large numbers of new psychoactive substances (NPS) have entered the drug market and these pose a worldwide problem to human health. Metabolomics approaches are useful tools for simultaneous detection of endogenous metabolites affected by drug use. They allow identification of pathways or characteristic metabolites, which might support the understanding of pharmacological actions or act as indirect biomarkers of consumption behavior or analytical detectability. Herein, we performed a comparative metabolic profiling of three psychoactive stimulant drugs 3,4-methylenedioxymethamphetamine (MDMA), amphetamine and the NPS mephedrone by liquid chromatography-high resolution mass spectrometry (LC-HRMS) in order to identify common pathways or compounds. Plasma samples were obtained from controlled administration studies to humans. Various metabolites were identified as increased or decreased based on drug intake, mainly belonging to energy metabolism, steroid biosynthesis and amino acids. Linoleic acid and pregnenolone-sulfate changed similarly in response to intake of all drugs. Overall, mephedrone produced a profile more similar to that of amphetamine than MDMA in terms of affected energy metabolism. These data can provide the basis for further in-depth targeted metabolome studies on pharmacological actions and search for biomarkers of drug use.

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Repeated Administration of D-Amphetamine Induces Distinct Alterations in Behavior and Metabolite Levels in 129Sv and Bl6 Mouse Strains

Cited by 12 publications

References 65 publications

Serum Metabolomic Analysis of Male Patients with Cannabis or Amphetamine Use Disorder

Serum Metabolomic Analysis of Male Patients with Cannabis or Amphetamine Use Disorder

Towards Extending the Detection Window of Gamma-Hydroxybutyric Acid—An Untargeted Metabolomics Study in Serum and Urine Following Controlled Administration in Healthy Men

Comparative Untargeted Metabolomics Analysis of the Psychostimulants 3,4-Methylenedioxy-Methamphetamine (MDMA), Amphetamine, and the Novel Psychoactive Substance Mephedrone after Controlled Drug Administration to Humans

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