Metabolic fingerprinting of rat urine by LC/MSPart 1. Analysis by hydrophilic interaction liquid chromatography–electrospray ionization mass spectrometry

Idborg, Helena; Zamani, Leila; Edlund, Per-Olof; Schuppe-Koistinen, Ina; Jacobsson, Sven P.

doi:10.1016/j.jchromb.2005.07.031

Cited by 145 publications

(103 citation statements)

References 13 publications

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“…Two main analytical techniques are predominantly reported and described in metabolomics studies: 1H nuclear magnetic resonance spectroscopy (NMR) (Nicholson et al 1999) and gaz or high-performance liquid chromatography coupled to mass spectrometry (Bajad and Shulaev 2007;Theodoris et al 2008). NMR which has traditionally been used in this context present clear advantages in terms of robustness and reproducibility but MS based methods have recently proved to be valuable for such studies, especially thanks to recent technological advances (Tolstikov and Fiehn 2002;Tolstikov et al 2003;Idborg et al 2005) and furthermore present some incomparable advantages over NMR either in terms of sensitivity or capabilities for structural elucidation purposes. Several ionization/desorption techniques have been used in MS metabolomics, including FAB (Platt et al 1988), MALDI (Jarman et al 2000) or Electrospray (McDougall et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Generation and processing of urinary and plasmatic metabolomic fingerprints to reveal an illegal administration of recombinant equine growth hormone from LC-HRMS measurements

et al. 2010

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Growth hormones are proteins produced by the anterior pituitary gland responsible for bone and tissue growth through their effects on carbohydrates, lipids and proteins metabolisms. Despite strict regulations banning the use of recombinant equine growth hormone, this substance is suspected to be misused to improve the horse physical performances. In order to check whether the regulation is fulfilled or not, controls are organized and a new analytical screening tool potentially able to detect such abuse was investigated in this paper. An untargeted metabolomics approach, based on liquid chromatography coupled to high resolution mass spectrometry, was developed and applied to characterize and compare horse urinary and plasmatic metabolic profiles upon reGH administrations. After minimal sample preparation, biological fluids were analyzed by LC-ESI(±)-Q-TOF. Data processing was performed by XCMS software and multivariate data analysis applied to the generated data set allowed building OPLS models to discriminate control versus treated populations. Results demonstrated significant metabolic modifications consecutively to the reGH treatment. A comparative study between urinary and plasmatic signatures was performed to evaluate the resulting metabolomic models and to asses their respective interests in the scope of real application for screening reGH administration.

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Section: Introductionmentioning

confidence: 99%

Generation and processing of urinary and plasmatic metabolomic fingerprints to reveal an illegal administration of recombinant equine growth hormone from LC-HRMS measurements

et al. 2010

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“…Recently an increasing number of publications have described HPLC-MS-based methods in metabonomic study, either alone (Plumb et al, 2002;Wilson et al, 2005b) or in combination with NMR (Lenz et al, 2004). Many reports demonstrate considerable promise for HPLC-MS-based strategies for metabonomic research (Yang et al, 2005;Idborg et al, 2005;Li et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

A pharmaco‐metabonomic study on the therapeutic basis and metabolic effects of Epimedium brevicornum Maxim. on hydrocortisone‐induced rat using UPLC‐MS

Li¹,

Lu²,

Liu³

et al. 2007

Biomedical Chromatography

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This paper describes the pharmaco-metabonomic study on Epimedium brevicornum Maxim. treated rats with a pathologic condition similar to the 'kidney deficiency syndromes' in traditional Chinese medicine and its therapeutic basis. UPLC-MS technique was used for the development of chemical profile of Epimedium brevicornum Maxim. and endogenous metabolite profiles of rats pre- and post-hydrocortisone interfered and treated with this herbal medicine. The comparison among profiles was performed with a statistical technique, principle component analysis (PCA). Significant difference in endogenous metabolite profiles was observed in the intervention rats and the abnormality of metabolism recovered towards the normal level after administration with Epimedium brevicornum Maxim. extract. Four active constituents of Epimedium brevicornum Maxim. were found into the blood circulation of kidney-deficient rats and two of their metabolites in the urine. This work suggests that the metabonomic approach is a potentially powerful tool to explore the therapeutic basis and to clarify the possible action mechanism of traditional Chinese medicine.

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“…HILIC is a new liquid chromatography mode that allows for the separation of highly polar compounds [26,27]. Since Idborg et al [28,29] first reported the HILIC-based metabolite profiling study of rat urine in 2005, there is a steady increase in reports about the applications of general high-performance HILIC/MS in metabolite profiling and metabonomic studies [25,[30][31][32][33][34][35][36][37]. Most of these studies used urine samples, which are relatively simple to prepare.…”

Section: Introductionmentioning

confidence: 99%

Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS

et al. 2011

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In this study, plasma samples were collected from 28 health volunteers and 66 lung cancer patients. Ultraperformance HILIC/Q-TOF MS-based metabonomic techniques were employed to compare the highly polar metabolites (HPMs) in the plasma of patients with lung cancer and those in the plasma of healthy volunteers. High separation efficiency and good repeatability of the HILIC/ Q-TOF MS method were observed. Partial least squares discriminant analysis (PLS-DA) of the data from both ESI? and ESI-mode revealed good classifications between the health volunteer and the lung cancer patients. This result showed that the HPMs from lung cancer patients and healthy controls were significantly different. The correct classification rate of models in both ion modes reached 100%, indicating that the metabonomics method established in this study could predict the plasma samples with high accuracy. Besides, we found 19 ions that show a significant difference in levels between lung cancer patients and healthy controls. Finally, we conducted a primary study of the effect of radiotherapy on HPMs in lung cancer patients. Clear differences among the HPMs of patients in different radiotherapy periods and significant changes in levels of the 19 ions after radiotherapy were noted. This demonstrated that radiotherapy influences the metabolism system of patients.

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Metabolic fingerprinting of rat urine by LC/MSPart 1. Analysis by hydrophilic interaction liquid chromatography–electrospray ionization mass spectrometry

Cited by 145 publications

References 13 publications

Generation and processing of urinary and plasmatic metabolomic fingerprints to reveal an illegal administration of recombinant equine growth hormone from LC-HRMS measurements

Generation and processing of urinary and plasmatic metabolomic fingerprints to reveal an illegal administration of recombinant equine growth hormone from LC-HRMS measurements

A pharmaco‐metabonomic study on the therapeutic basis and metabolic effects of Epimedium brevicornum Maxim. on hydrocortisone‐induced rat using UPLC‐MS

Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS

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