Metabolic engineering of soybean seeds for enhanced vitamin E tocochromanol content and effects on oil antioxidant properties in polyunsaturated fatty acid-rich germplasm

Konda, Anji Reddy; Nazarenus, Tara J.; Nguyen, Hanh; Yang, Junsi; Gelli, Malleswari; Swenson, Samantha; Shipp, Jamie M.; Schmidt, Monica A.; Cahoon, Rebecca E.; Ciftci, Ozan N.; Zhang, Chunyu; Clemente, Tom; Cahoon, Edgar B.

doi:10.1016/j.ymben.2019.10.005

Cited by 29 publications

(31 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As expected, higher yields of γ/β-tocotrienol and α-tocotrienol were obtained. Although overexpression of the Arabidopsis γ-TMT in plant seeds resulted in the near-complete conversion of γtocopherol to α-tocopherol 57,62 and γ-tocotrienol to αtocotrienol 15 , the activity of γ-TMT seemed to be low when expressed in yeast, with γ-tocotrienol remaining as the main product. After comprehensive optimization of the whole pathway, the tocotrienols yield was improved to 7.6 mg/g DCW.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, natural tocotrienols are mainly derived from crude palm oil which contains up to 800 mg/kg of tocotrienols 13 . Recently, metabolic engineering efforts in soybean seeds have turned soybean into a viable platform for producing high levels of vitamin E tocochromanols 14 , 15 . Meanwhile, recent advances in synthetic biology have enabled heterologous biosynthesis of various natural products in model microorganisms 16 – 19 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fermentative production of Vitamin E tocotrienols in Saccharomyces cerevisiae under cold-shock-triggered temperature control

et al. 2020

View full text Add to dashboard Cite

The diverse physiological functions of tocotrienols have listed them as valuable supplementations to α-tocopherol-dominated Vitamin E products. To make tocotrienols more readily available, tocotrienols-producing S. cerevisiae has been constructed by combining the heterologous genes from photosynthetic organisms with the endogenous shikimate pathway and mevalonate pathway. After identification and elimination of metabolic bottlenecks and enhancement of precursors supply, the engineered yeast can produce tocotrienols at yield of up to 7.6 mg/g dry cell weight (DCW). In particular, proper truncation of the N-terminal transit peptide from the plant-sourced enzymes is crucial. To further solve the conflict between cell growth and tocotrienols accumulation so as to enable high-density fermentation, a cold-shock-triggered temperature control system is designed for efficient control of two-stage fermentation, leading to production of 320 mg/L tocotrienols. The success in high-density fermentation of tocotrienols by engineered yeast sheds light on the potential of fermentative production of vitamin E tocochromanols.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fermentative production of Vitamin E tocotrienols in Saccharomyces cerevisiae under cold-shock-triggered temperature control

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Nowadays, structure and role of about 400 different fatty acids are known in the plant kingdom [4]. Some of them are inevitable in the proper function of plant cells and some have positive effects on human health (e.g., antiinflammatory [5][6][7], anticancer [8,9], antibacterial [10], and antiparasitic activity [11]) or are demanded in the different branches of industry, like food, pharmaceuticals, and cosmetics production [12][13][14].…”

Section: Classification Of Fatty Acids In Plantsmentioning

confidence: 99%

Lipids Composition in Plant Membranes

Reszczyńska

Hanaka

2020

Cell Biochem Biophys

116

View full text Add to dashboard Cite

The paper focuses on the selected plant lipid issues. Classification, nomenclature, and abundance of fatty acids was discussed. Then, classification, composition, role, and organization of lipids were displayed. The involvement of lipids in xantophyll cycle and glycerolipids synthesis (as the most abundant of all lipid classes) were also discussed. Moreover, in order to better understand the biomembranes remodeling, the model (artificial) membranes, mimicking the naturally occurring membranes are employed and the survey on their composition and application in different kind of research was performed. High level of lipids remodeling in the plant membranes under different environmental conditions, e.g., nutrient deficiency, temperature stress, salinity or drought was proved. The key advantage of lipid research was the conclusion that lipids could serve as the markers of plant physiological condition and the detailed knowledge on lipids chemistry will allow to modify their composition for industrial needs.

show abstract

“…One can distinguish two major groups of vitEs in plants called tocopherol and tocotrienol, both of which have antioxidative capacities [ 177 , 178 , 179 , 180 , 181 ]. Tocopherol and tocotrienol share a chromane ring and mainly differ in their hydrophobic tails ( Figure 6 a).…”

Section: Vitamin-based Antioxidantsmentioning

confidence: 99%

Antioxidants in Potatoes: A Functional View on One of the Major Food Crops Worldwide

2021

View full text Add to dashboard Cite

With a growing world population, accelerating climate changes, and limited arable land, it is critical to focus on plant-based resources for sustainable food production. In addition, plants are a cornucopia for secondary metabolites, of which many have robust antioxidative capacities and are beneficial for human health. Potato is one of the major food crops worldwide, and is recognized by the United Nations as an excellent food source for an increasing world population. Potato tubers are rich in a plethora of antioxidants with an array of health-promoting effects. This review article provides a detailed overview about the biosynthesis, chemical and health-promoting properties of the most abundant antioxidants in potato tubers, including several vitamins, carotenoids and phenylpropanoids. The dietary contribution of diverse commercial and primitive cultivars are detailed and document that potato contributes much more than just complex carbohydrates to the diet. Finally, the review provides insights into the current and future potential of potato-based systems as tools and resources for healthy and sustainable food production.

show abstract

Metabolic engineering of soybean seeds for enhanced vitamin E tocochromanol content and effects on oil antioxidant properties in polyunsaturated fatty acid-rich germplasm

Cited by 29 publications

References 55 publications

Fermentative production of Vitamin E tocotrienols in Saccharomyces cerevisiae under cold-shock-triggered temperature control

Fermentative production of Vitamin E tocotrienols in Saccharomyces cerevisiae under cold-shock-triggered temperature control

Lipids Composition in Plant Membranes

Antioxidants in Potatoes: A Functional View on One of the Major Food Crops Worldwide

Contact Info

Product

Resources

About