Metabolic engineering of Saccharomyces cerevisiae for the production of top value chemicals from biorefinery carbohydrates

Baptista, Sara Isabel Leite; Costa, Carlos E.; Cunha, Joana T.; Soares, Pedro; Domingues, Lucı́lia

doi:10.1016/j.biotechadv.2021.107697

Cited by 76 publications

(35 citation statements)

References 204 publications

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“…The cell growth, lactic acid production, and ethanol production of BK01 were significantly better than SR8LDH under the acetic acid stress. Lactic acid production by engineered S. cerevisiae was demonstrated mostly using complex and synthetic medium [50], and cellulosic lactic acid production was reported only in a few recent studies [8,51]. Using spent coffee ground hydrolysates and wheat straw hydrolysates, 11.5 g/L [8] and 10 g/L [51] lactic acid were produced.…”

Section: Discussionmentioning

confidence: 99%

l-Lactic Acid Production Using Engineered Saccharomyces cerevisiae with Improved Organic Acid Tolerance

Jang

Jeong

et al. 2021

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Lactic acid is mainly used to produce bio-based, bio-degradable polylactic acid. For industrial production of lactic acid, engineered Saccharomyces cerevisiae can be used. To avoid cellular toxicity caused by lactic acid accumulation, pH-neutralizing agents are used, leading to increased production costs. In this study, lactic acid-producing S. cerevisiae BK01 was developed with improved lactic acid tolerance through adaptive laboratory evolution (ALE) on 8% lactic acid. The genetic basis of BK01 could not be determined, suggesting complex mechanisms associated with lactic acid tolerance. However, BK01 had distinctive metabolomic traits clearly separated from the parental strain, and lactic acid production was improved by 17% (from 102 g/L to 119 g/L). To the best of our knowledge, this is the highest lactic acid titer produced by engineered S. cerevisiae without the use of pH neutralizers. Moreover, cellulosic lactic acid production by BK01 was demonstrated using acetate-rich buckwheat husk hydrolysates. Particularly, BK01 revealed improved tolerance against acetic acid of the hydrolysates, a major fermentation inhibitor of lignocellulosic biomass. In short, ALE with a high concentration of lactic acid improved lactic acid production as well as acetic acid tolerance of BK01, suggesting a potential for economically viable cellulosic lactic acid production.

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Section: Discussionmentioning

confidence: 99%

l-Lactic Acid Production Using Engineered Saccharomyces cerevisiae with Improved Organic Acid Tolerance

Jang

Jeong

et al. 2021

JoF

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“…The yeast S. cerevisiae, being the preferred microorganism for the production of bioethanol, has been the most explored microorganism for application in CBP processes. In addition, this yeast plays a central role in lignocellulosic valorization processes not only to bioethanol [104] but also to top chemicals [105] due to its tolerance to adverse lignocellulose-based process conditions [106]. Taking advantage of the extensive genetic toolbox available, a wide variety of modifications has been applied to this yeast in order to provide it with efficient cellulolytic activity, following three distinct approaches: enzyme secretion, cellulosomes and cell surface display [104].…”

Section: Engineering Ethanologenic Microorganisms For Cellulase Productionmentioning

confidence: 99%

Strategies towards Reduction of Cellulases Consumption: Debottlenecking the Economics of Lignocellulosics Valorization Processes

et al. 2021

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Lignocellulosic residues have been receiving growing interest as a promising source of polysaccharides, which can be converted into a variety of compounds, ranging from biofuels to bioplastics. Most of these can replace equivalent products traditionally originated from petroleum, hence representing an important environmental advantage. Lignocellulosic materials are theoretically unlimited, cheaper and may not compete with food crops. However, the conversion of these materials to simpler sugars usually requires cellulolytic enzymes. Being still associated with a high cost of production, cellulases are commonly considered as one of the main obstacles in the economic valorization of lignocellulosics. This work provides a brief overview of some of the most studied strategies that can allow an important reduction of cellulases consumption, hence improving the economy of lignocellulosics conversion. Cellulases recycling is initially discussed regarding the main processes to recover active enzymes and the most important factors that may affect enzyme recyclability. Similarly, the potential of enzyme immobilization is analyzed with a special focus on the contributions that some elements of the process can offer for prolonged times of operation and improved enzyme stability and robustness. Finally, the emergent concept of consolidated bioprocessing (CBP) is also described in the particular context of a potential reduction of cellulases consumption.

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“…S. cerevisiae is a GRAS microorganism widely used for bioethanol production (Cunha et al, 2020), being also applied in the production of valueadded chemicals (Baptista et al, 2021). The existing genetic toolbox allied to its high fermentative capacity facilitates the use of this yeast as a microbial cell factory.…”

Section: Exploiting Saccharomyces Cerevisiae For Cheese Whey-to-ethanol Valorisationmentioning

confidence: 99%

“…Some yeast strains, such as Kluyveromyces lactis, Kluyveromyces marxianus, Kluyveromyces fragilis (now considered a K. marxianus species, accordingly to the current nomenclature), and Candida pseudotropicalis, have been used as platform cell factories due to their natural ability to consume lactose (Guimarães et al, 2010). The yeast Saccharomyces cerevisiae, widely recognized as an ideal microbial cell factory to produce bioethanol (Cunha et al, 2020) and to be used in lignocellulosic biorefineries (Baptista et al, 2021), can also be tailored for the valorisation of cheese whey through lactose consumption (Guimarães et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Yeast cell factories for sustainable whey-to-ethanol valorisation towards a circular economy

et al. 2021

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Cheese whey is the major by-product of the dairy industry, and its disposal constitutes an environmental concern. The production of cheese whey has been increasing, with 190 million tonnes per year being produced nowadays. Therefore, it is emergent to consider different routes for cheese whey utilization. The great nutritional value of cheese whey turns it into an attractive substrate for biotechnological applications. Currently, cheese whey processing includes a protein fractionating step that originates the permeate, a lactose-reach stream further used for valorisation. In the last decades, yeast fermentation has brought several advances to the search for biorefinery alternatives. From the plethora of value-added products that can be obtained from cheese whey, ethanol is the most extensively explored since it is the alternative biofuel most used worldwide. Thus, this review focuses on the different strategies for ethanol production from cheese whey using yeasts as promising biological systems, including its integration in lignocellulosic biorefineries. These valorisation routes encompass the improvement of the fermentation process as well as metabolic engineering techniques for the introduction of heterologous pathways, resorting mainly to Kluyveromyces sp. and Saccharomyces cerevisiae strains. The solutions and challenges of the several strategies will be unveiled and explored in this review.

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Metabolic engineering of Saccharomyces cerevisiae for the production of top value chemicals from biorefinery carbohydrates

Cited by 76 publications

References 204 publications

l-Lactic Acid Production Using Engineered Saccharomyces cerevisiae with Improved Organic Acid Tolerance

l-Lactic Acid Production Using Engineered Saccharomyces cerevisiae with Improved Organic Acid Tolerance

Strategies towards Reduction of Cellulases Consumption: Debottlenecking the Economics of Lignocellulosics Valorization Processes

Yeast cell factories for sustainable whey-to-ethanol valorisation towards a circular economy

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