l-Lactic Acid Production Using Engineered Saccharomyces cerevisiae with Improved Organic Acid Tolerance

Jang, Byeong‐Kwan; Ju, Yebin; Jeong, Deokyeol; Jung, Sung-Keun; Kim, Chang-Kil; Chung, Yong‐Suk; Kim, Soo Rin

doi:10.3390/jof7110928

Cited by 19 publications

(7 citation statements)

References 50 publications

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“…Even though the accumulation of lactic acid declined under NN condition, the removal of calcium carbonate did not cause a significant decrease in the rate of glucose uptake as the glucose was completely consumed within only 9 h under both SN and NN conditions. Nonetheless, as shown in Table 2, these results were relatively competitive if compared with the productivity of other microbial hosts as previously reported 25,[34][35][36][37] . Despite its lower titer and yield, the LA2 strain maintained the ability to utilize glucose rapidly and exhibited a lower drop in productivity under NN condition compared to other studies.…”

Section: Resultssupporting

confidence: 82%

Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization

Pangestu

Kahar

Kholida

et al. 2022

Sci Rep

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Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L−1 h−1 under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L−1 h−1, which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient.

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Section: Resultssupporting

confidence: 82%

Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization

Pangestu

Kahar

Kholida

et al. 2022

Sci Rep

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“…The l -LDH from B. coagulans was first successfully expressed and showed better synthetic ability for l -lactic acid production ( Fig. 6B ) than the l -LDH from other bacteria ( 58 ), fungi ( 59 ), and mammals ( 60 ) reported in S. cerevisiae . This provides an optional superior l -LDH that promotes l -lactic acid fermentation in other yeasts.…”

Section: Discussionmentioning

confidence: 99%

“…S16). Fermentation at low pH values decreases the use of neutralizing agents ( 58 ). This means that our strains and medium are more economical, reducing the cost of l -lactic acid fermentation.…”

Section: Discussionmentioning

confidence: 99%

A robust yeast chassis: comprehensive characterization of a fast-growing Saccharomyces cerevisiae

Long,

Han,

Meng

et al. 2024

mBio

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Robust chassis are critical to facilitate advances in synthetic biology. This study describes a comprehensive characterization of a new yeast isolate Saccharomyces cerevisiae XP that grows faster than commonly used research and industrial S. cerevisiae strains. The genomic, transcriptomic, and metabolomic analyses suggest that the fast growth rate is, in part, due to the efficient electron transport chain and key growth factor synthesis. A toolbox for genetic manipulation of the yeast was developed; we used it to construct l -lactic acid producers for high lactate production. The development of genetically malleable yeast strains that grow faster than currently used strains may significantly enhance the uses of S. cerevisiae in biotechnology. IMPORTANCE Yeast is known as an outstanding starting strain for constructing microbial cell factories. However, its growth rate restricts its application. A yeast strain XP, which grows fast in high concentrations of sugar and acidic environments, is revealed to demonstrate the potential in industrial applications. A toolbox was also built for its genetic manipulation including gene insertion, deletion, and ploidy transformation. The knowledge of its metabolism, which could guide the designing of genetic experiments, was generated with multi-omics analyses. This novel strain along with its toolbox was then tested by constructing an l -lactic acid efficient producer, which is conducive to the development of degradable plastics. This study highlights the remarkable competence of nonconventional yeast for applications in biotechnology.

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“…In contrast, the LA-producing S. cerevisiae BK01, constructed and evolved by Jang et al [ 78 ], reached virtually the same titer as the S.c-NO.2-100, 119 g/L in fed-batch mode, but without using any neutralizing agent during cultivation. The BK01 strain was generated by evolving a previously described xylose-consuming strain (SR8L) in several subcultures on 8% LA [ 82 ].…”

Section: Improving Acidity Tolerance In Yeast and Its Influence On La...mentioning

confidence: 99%

Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production

Melo,

de Oliveira Junqueira,

Lima

et al. 2024

JoF

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Lactic acid (LA) production has seen significant progress over the past ten years. LA has seen increased economic importance due to its broadening use in different sectors such as the food, medicine, polymer, cosmetic, and pharmaceutical industries. LA production bioprocesses using microorganisms are economically viable compared to chemical synthesis and can benefit from metabolic engineering for improved productivity, purity, and yield. Strategies to optimize LA productivity in microorganisms on the strain improvement end include modifying metabolic routes, adding gene coding for lactate transporters, inducing tolerance to organic acids, and choosing cheaper carbon sources as fuel. Many of the recent advances in this regard have involved the metabolic engineering of yeasts and filamentous fungi to produce LA due to their versatility in fuel choice and tolerance of industrial-scale culture conditions such as pH and temperature. This review aims to compile and discuss metabolic engineering innovations in LA production in yeasts and filamentous fungi over the 2013–2023 period, and present future directions of research in this area, thus bringing researchers in the field up to date with recent advances.

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l-Lactic Acid Production Using Engineered Saccharomyces cerevisiae with Improved Organic Acid Tolerance

Cited by 19 publications

References 50 publications

Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization

Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization

A robust yeast chassis: comprehensive characterization of a fast-growing Saccharomyces cerevisiae

Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production

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