Metabolic engineering of microorganisms for the production of multifunctional non‐protein amino acids: γ‐aminobutyric acid and δ‐aminolevulinic acid

Su, Anping; Yu, Qijun; Ying, Luo; Yang, Jinshui; Wang, En Tao; Yuan, Hongli

doi:10.1111/1751-7915.13783

Cited by 11 publications

(12 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surprisingly, the products of the Δstc27 mutant showed significant growth‐promoting activity. This indicates that STE biosynthesis probably competes with some biosynthesis pathway of natural products (Min et al, 2019 ; Su et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

New application of Aspergillus versicolor in promoting plant growth after suppressing sterigmatocystin production via genome mining and engineering

Zhao

Han

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

Aspergillus genus is a key component in fermentation and food processing. However, sterigmatocystin (STE)-a mycotoxin produced by several species of Aspergillus-limits the use of some Aspergillus species (such as Aspergillus versicolor, Aspergillus inflatus, and Aspergillus parasiticus) because of its toxicity and carcinogenicity. Here, we engineered an STE-free Aspergillus versicolor strain based on genome mining techniques. We sequenced and assembled the Aspergillus versicolor D5 genome (34.52 Mb), in which we identified 16 scaffolds and 54 biosynthetic gene clusters (BGCs). We silenced cytochrome P450 coding genes STC17 and STC27 by insertional inactivation. The production of STE in the Δstc17 mutant strain was increased by 282% but no STE was detected in the Δstc27 mutant. Metabolites of Δstc27 mutant exhibited growth-promoting effect on plants. Our study makes significant progress in improving the application of some Aspergillus strains by restricting their production of toxic and carcinogenic compounds.

show abstract

Section: Discussionmentioning

confidence: 99%

New application of Aspergillus versicolor in promoting plant growth after suppressing sterigmatocystin production via genome mining and engineering

Zhao

Han

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

show abstract

“…Currently, the commercial production of ALA relies on chemical synthesis and the complex synthesis reactions and low production efficiency led to the high price of ALA (Kang et al, 2017 ; Su et al, 2021 ). Microbial production of ALA is an alternative to chemical synthesis and might have advantages in economical practicability and sustainability (Kang et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

“…In microorganisms, ALA can be synthesized via two natural pathways, including the C4 pathway in purple non‐sulphur photosynthetic bacteria ( Rhodobacter sphaeroides ) (Kang et al, 2017 ), and the C5 pathway in many bacteria, such as Escherichia coli (Li et al, 1989 ) and Salmonella typhimurium (Wang et al, 1999 ). Previous studies had mainly focused on increasing ALA production efficiency by engineering E. coli and Corynebacterium glutamicum strains (Su et al, 2021 ). One bioengineering strategy was the heterologous expression of alaS gene (encoding ALA synthase) from R. sphaeroides into E. coli (Zhu et al, 2019 ) or C. glutamicum (Feng et al, 2016 ; Yang et al, 2016 ) to produce ALA via C4 pathway.…”

Section: Introductionmentioning

confidence: 99%

“…To date, glucose as a carbon source (Su et al, 2021 ), and tryptone, yeast extract (Yu et al, 2015 ) or ammonium sulphate (Zhang et al, 2019 ) as nitrogen sources had been used in almost all the reported ALA production procedures, in which addition of inducers and antibiotics were also required for the engineering of strains. The high cost of these procedures limited ALA microbial production and application (Chen et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Production of 5‐aminolevulinic acid from hydrolysates of cassava residue and fish waste by engineered Bacillus cereusPT1

Ying

Yang

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

The economical production of 5‐aminolevulinic acid (ALA) has recently received increasing attention for its extensive use in agriculture. In this study, a strain of Bacillus cereus PT1 could initially produce ALA at a titre of 251.72 mg/L by using a hydrolysate mixture of low‐cost cassava residue and fish waste. The integration of endogenous hemA encoding glutamyl‐tRNA reductase led to a 39.30% increase in ALA production. Moreover, improving cell permeability by deletion of the LytR‐CpsA‐Psr (LCP) family gene tagU led to a further increase of 59.73% in ALA production. Finally, the engineered strain B. cereus PT1‐ hemA ‐Δ tagU produced 2.62 g/L of ALA from the previously mentioned hydrolysate mixture in a 7‐L bioreactor. In a pot experiment, foliar spray of the ALA produced by B. cereus PT1‐ hemA ‐Δ tagU from the hydrolysates increased salt tolerance of cucumber by improving chlorophyll content and catalase activity, while decreasing malondialdehyde content. Overall, this study demonstrated an economic way to produce ALA using a microbial platform and evidenced the potential of ALA in agricultural application.

show abstract

“…5-aminolevulinic acid (5-ALA) is a natural non-protein amino acid that exists as a common precursor of heme in animals [ 1 ]. Endogenous 5-ALA is synthesized from succinate coenzyme A and glycine catalyzed by the 5-aminolevulinate synthase.…”

Section: Introductionmentioning

confidence: 99%

Evolutionary Trend Analysis of Research on 5-ALA Delivery and Theranostic Applications Based on a Scientometrics Study

Zhou

Luo

et al. 2022

Pharmaceutics

View full text Add to dashboard Cite

5-aminolevulinic acid (5-ALA) has been extensively studied for its sustainability and broad-spectrum applications in medical research and theranostics, as well as other areas. It’s a precursor of protoporphyrin IX (PpIX), a sustainable endogenous and naturally-existing photosensitizer. However, to the best of our knowledge, a scientometrics study based on the scientific knowledge assay of the overall situation on 5-ALA research has not been reported so far, which would be of major importance to the relevant researchers. In this study, we collected all the research articles published in the last two decades from the Web of Science Core Collection database and employed bibliometric methods to comprehensively analyze the dataset from different perspectives using CiteSpace. A total of 1595 articles were identified. The analysis results showed that China published the largest number of articles, and SBI Pharmaceuticals Co., Ltd. was the most productive institution that sponsored several of the most productive authors. The cluster analysis and burst detections indicated that the improvement of photodynamic efficacy theranostics is the up-to-date key direction in 5-ALA research. Furthermore, we emphatically studied nanotechnology involvement in 5-ALA delivery and theranostics research. We envision that our results will be beneficial for researchers to have a panorama of and deep insights into this area, thus inspiring further exploitations, especially of the nanomaterial-based systems for 5-ALA delivery and theranostic applications.

show abstract

Metabolic engineering of microorganisms for the production of multifunctional non‐protein amino acids: γ‐aminobutyric acid and δ‐aminolevulinic acid

Cited by 11 publications

References 54 publications

New application of Aspergillus versicolor in promoting plant growth after suppressing sterigmatocystin production via genome mining and engineering

New application of Aspergillus versicolor in promoting plant growth after suppressing sterigmatocystin production via genome mining and engineering

Production of 5‐aminolevulinic acid from hydrolysates of cassava residue and fish waste by engineered Bacillus cereusPT1

Evolutionary Trend Analysis of Research on 5-ALA Delivery and Theranostic Applications Based on a Scientometrics Study

Contact Info

Product

Resources

About