Metabolic engineering of Corynebacterium glutamicum for the de novo production of ethylene glycol from glucose

Chen, Zhen; Huang, Jinhai; Wu, Yao‐Wen; Liu, Dehua

doi:10.1016/j.ymben.2015.10.013

Cited by 53 publications

(48 citation statements)

References 40 publications

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“…Ethylene glycol and glycolic acid synthesis by E. cloacae started after ~ 10 h of cultivation, and entered a high rate after around 12 h. However, cell growth rate was highest between [3][4][5][6][7][8][9][10][11][12]5,6,7,8). Thus cell growth and the synthesis of ethylene glycol and glycolic acid were not coincided.…”

Section: Ethylene Glycol Production By E Cloacaementioning

confidence: 93%

“…In another study 40 g/L ethylene glycol was produced with a yield of 0.63 g/g xylose and productivity of 0.55 g/L h after some optimization of the conditions [4]. Using glucose as substrate, 3.5 g/L ethylene glycol was produced by engineering C. glutamicum, with a yield of 0.08 g/g glucose and productivity of 0.05 g/L h [5]. Using E. coli as the host cell, 4.1 g/L ethylene glycol was produced with a yield of 0.14 g/g glucose and productivity of 0.03 g/L h were obtained [6].…”

Section: Ethylene Glycol Production By E Cloacaementioning

confidence: 99%

“…Beside pentose, glucose was also used for ethylene glycol production. This synthesis pathway was constructed in Corynebacterium glutamicum and E. coli by using serine as an intermediate [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae

et al. 2020

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Background: Biological routes for ethylene glycol production have been developed in recent years by constructing the synthesis pathways in different microorganisms. However, no microorganisms have been reported yet to produce ethylene glycol naturally.Results: Xylonic acid utilizing microorganisms were screened from natural environments, and an Enterobacter cloacae strain was isolated. The major metabolites of this strain were ethylene glycol and glycolic acid. However, the metabolites were switched to 2,3-butanediol, acetoin or acetic acid when this strain was cultured with other carbon sources. The metabolic pathway of ethylene glycol synthesis from xylonic acid in this bacterium was identified. Xylonic acid was converted to 2-dehydro-3-deoxy-d-pentonate catalyzed by d-xylonic acid dehydratase. 2-Dehydro-3-deoxy-d-pentonate was converted to form pyruvate and glycolaldehyde, and this reaction was catalyzed by an aldolase. d-Xylonic acid dehydratase and 2-dehydro-3-deoxy-d-pentonate aldolase were encoded by yjhG and yjhH, respectively. The two genes are part of the same operon and are located adjacent on the chromosome. Besides yjhG and yjhH, this operon contains four other genes. However, individually inactivation of these four genes had no effect on either ethylene glycol or glycolic acid production; both formed from glycolaldehyde. YqhD exhibits ethylene glycol dehydrogenase activity in vitro. However, a low level of ethylene glycol was still synthesized by E. cloacae ΔyqhD. Fermentation parameters for ethylene glycol and glycolic acid production by the E. cloacae strain were optimized, and aerobic cultivation at neutral pH were found to be optimal. In fed batch culture, 34 g/L of ethylene glycol and 13 g/L of glycolic acid were produced in 46 h, with a total conversion ratio of 0.99 mol/mol xylonic acid. Conclusions:A novel route of xylose biorefinery via xylonic acid as an intermediate has been established.

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Section: Ethylene Glycol Production By E Cloacaementioning

confidence: 93%

Section: Ethylene Glycol Production By E Cloacaementioning

confidence: 99%

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Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae

et al. 2020

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Section: Ethylene Glycol and Glycolic Acid Synthesis Have An Inherentmentioning

confidence: 99%

Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae

Zhang

Yang

Wang

et al. 2019

Preprint

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“…Currently, EG is mainly produced by chemical synthesis that proceeds through following three steps: steam cracking of petrol, oxidation of ethylene, and thermal hydrolysis of ethylene oxide. As demand for sustainable chemical production increases, several engineered microbes have been developed for EG production from renewable carbon sources such as glucose, xylose, d ‐arabinose, l ‐lyxose, and l ‐arabinose . Among them, most efficient EG production was achieved using xylose as a carbon source.…”

Section: Introductionmentioning

confidence: 99%

Production of ethylene glycol from xylose by metabolically engineered Escherichia coli

Chae

Choi

Ryu³

et al. 2018

AIChE Journal

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Ethylene glycol (EG) is an important chemical used for several industrial applications including poly(ethylene terephthalate) synthesis. In this study, Escherichia coli was metabolically engineered to efficiently produce EG from xylose. To biosynthesize EG, the Dahms pathway was introduced by expressing xylBC genes from Caulobacter crescentus (xylBCccs). Various E. coli strains and glycolaldehyde reductases were screened to find E. coli W3110 strain and glycolaldehyde reductase (yqhD) as optimal combination for EG production. In silico genome‐scale metabolic simulation suggested that increasing the native xylose pathway flux, in the presence of the overexpressed Dahms pathway, is beneficial for EG production. This was achieved by reducing the Dahms pathway flux by employing a synthetic small regulatory RNA targeting xylBccs. Fed‐batch culture of the final engineered E. coli strain produced 108.2 g/L of EG in a xylose minimal medium. The yield on xylose and EG productivity were 0.36 g/g (0.87 mol/mol) and 2.25 g/L/h, respectively. © 2018 American Institute of Chemical Engineers AIChE J, 64: 4193–4200, 2018

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Metabolic engineering of Corynebacterium glutamicum for the de novo production of ethylene glycol from glucose

Cited by 53 publications

References 40 publications

Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae

Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae

Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae

Production of ethylene glycol from xylose by metabolically engineered Escherichia coli

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