Metabolic engineering of a novel strain of electrogenic bacterium Arcobacter butzleri to create a platform for single analyte detection using a microbial fuel cell

Szydlowski, Lukasz; Lan, Tammy C. T.; Shibata, Noriko; Goryanin, Igor

doi:10.1016/j.enzmictec.2020.109564

Cited by 16 publications

(14 citation statements)

References 51 publications

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“…Strain ED-1 is the exception, as it can grow in the presence of lactate and acetate and moderately in the presence of succinate. Therefore, A. butzleri strains vary in terms of their organic acid metabolism ( 39 , 40 ). The metabolic pathways of human cells are also influenced by acetate and lactate concentrations ( 41 ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis of Arcobacter butzleri Infection in a Mucus-Producing Human Intestinal In Vitro Model

et al. 2023

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Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis of Arcobacter butzleri Infection in a Mucus-Producing Human Intestinal In Vitro Model

et al. 2023

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“…Samples from Okinawa mangrove forests (M), as described in Szydlowski et al ([16]), and air-conditioning unit outflow in Ishikawa, Japan (26.43°N, 127.84°E; AC) were mixed (1:3) with basal medium containing 200 mg/L CaCl 2 •2H 2 O, 250 mg/L MgCl 2 •6H 2 O, 500 mg/L NH 4 Cl, trace elements and vitamins solution (medium 141 DSMZ) with 1g/L acetate and 2 g/L lactate as carbon source was used, with pH adjusted to either 4 or 7, and incubated overnight at 25°C. To inoculate 96-well plate, five carbon veil (7 gsm, Elite Motoring, USA) disks (0.385 cm 2 each) were immersed in each solution overnight and transferred to the 96-well plate as indicated in Fig.1c.…”

Section: Methodsmentioning

confidence: 99%

High-throughput screening and selection of PCB-bioelectrocholeaching, electrogenic microbial communities using single chamber microbial fuel cells based on 96-well plate array

Szydlowski

Ehlich

Shibata

et al. 2021

Preprint

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We demonstrate a single chamber, 96-well plate based Microbial Fuel Cells (MFCs). This invention is aimed at robust selection of electrogenic microbial community under specific conditions, (pH, external resistance, inoculum) that can be altered within the 96 well plate array. Using this device, we selected and multiplicated electrogenic microbial communities fed with acetate and lactate that can operate under different pH and produce current densities up to 19.4 A/m3 (0.6 A/m2) within 5 days past inoculation. Moreover, studies shown that Cu mobilization through PCB bioleaching occurred, thus each community was able to withstand presence of Cu2+ ions up to 600 mg/L. Metagenome analysis reveals high abundance of Dietzia spp., previously characterized in MFCs, but not reported to grow at pH 4, as well as novel species, closely related to Actinotalea ferrariae, not yet associated with electrogenicity. Microscopic observations (combined SEM and EDS) reveal that some of the species present in the anodic biofilm were adsorbing copper on their surface, probably due to the presence of metalloprotein complexes on their outer membranes. Taxonomy analysis indicated that similar consortia populate anodes, cathodes and OCP controls, although total abundances of aforementioned species are different among those groups. Annotated metagenomes showed high presence of multicopper oxidases and Cu-resistance genes, as well as genes encoding aliphatic and aromatic hydrocarbon-degrading enzymes. Comparison between annotated and binned metagenomes from pH 4 and 7 anodes, as well as their OCP controls revealed unique genes present in all of them, with majority of unique genes present in pH 7 anode, where novel Actinotalea spp. was present.

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“…have engineered Arcobacter butzleri to either only produce current in the presence of acetate or only produce current in the presence of lactate. [ 14 ] To achieve this, they used mutants of the electroactive species A. butzleri lacking either the acetate or lactate oxidation pathway, therefore limiting each mutant to oxidation of a sole carbon source. The current production by A. butzleri could then be correlated to either acetate or lactate with an R 2 of 0.95.…”

Section: Synthetic Biology For Sensingmentioning

confidence: 99%

“…The current production by A. butzleri could then be correlated to either acetate or lactate with an R 2 of 0.95. [14] Similarly, the carbon source arabinose was sensed by a genetically engineered strain of wellstudied exoelectrogen S. oneidensis by Golitsch et al [15] This study used S. oneidensis mutants deficient in the Mtr pathway responsible for electroactivity in conjunction with a plasmid containing the Mtr pathway regulated by an arabinose-dependent promoter. This genetic construction expressed the Mtr pathway in the presence of arabinose and resulted in the restoration of electricity production.…”

Section: Synthetic Biology For Sensingmentioning

confidence: 99%

Applying synthetic biology strategies to bioelectrochemical systems

Dong

Simoska

Gaffney

2021

Electrochemical Science Adv

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Although the past 20 years have seen significant advances in tailoring materials for improving the performance of bioelectrochemical systems, recently, there have been efforts in utilizing the synthetic biology toolkit for engineering organisms for bioelectrochemical systems. This review discusses the use of synthetic biology to engineer non-native properties into bioelectrochemical systems for increasing the diversity of fuel utilization in energy applications, allowing for novel electrosynthetic strategies, and improving the selectivity of biosensors. The review also discusses synthetic biology strategies for improving the abiotic/biotic interface, which improves the performance of bioelectrochemical systems. Both strategies are required and need to be combined with materials innovation to produce commercially viable bioelectrochemical systems in the future.

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Metabolic engineering of a novel strain of electrogenic bacterium Arcobacter butzleri to create a platform for single analyte detection using a microbial fuel cell

Cited by 16 publications

References 51 publications

Transcriptome Analysis of Arcobacter butzleri Infection in a Mucus-Producing Human Intestinal In Vitro Model

Transcriptome Analysis of Arcobacter butzleri Infection in a Mucus-Producing Human Intestinal In Vitro Model

High-throughput screening and selection of PCB-bioelectrocholeaching, electrogenic microbial communities using single chamber microbial fuel cells based on 96-well plate array

Applying synthetic biology strategies to bioelectrochemical systems

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