Transcriptome Analysis of Arcobacter butzleri Infection in a Mucus-Producing Human Intestinal
            <i>In Vitro</i>
            Model

Buzzanca, Davide; Alessandria, Valentina; Botta, Cristian; Zadeh, Negin Seif; Ferrocino, Ilario; Houf, Kurt; Cocolin, Luca Simone; Rantsiou, Kalliopi

doi:10.1128/spectrum.02071-22

Cited by 6 publications

(4 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Orthogroups related to motA , tolQ, exbB, exbD and tonB (TonB functions) are negatively related to group 1 and absent in all these species. However, another orthogroup encoding for MotA/TolQ/ExbB proton channel family results positively correlated with group 1 suggesting a specific function of different orthologues related to different Arcobacter species as already suggested for A. butzleri [ 16 , 78 ]. Moreover, another positively correlated orthogroup is linked to β-lactamase activity, present in all species of group 1 indicating antibiotic resistance-related genes [ 79 ].…”

Section: Resultsmentioning

confidence: 58%

Arcobacteraceae comparative genome analysis demonstrates genome heterogeneity and reduction in species isolated from animals and associated with human illness

Buzzanca

Kerkhof

Alessandria

et al. 2023

Heliyon

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 58%

Arcobacteraceae comparative genome analysis demonstrates genome heterogeneity and reduction in species isolated from animals and associated with human illness

Buzzanca

Kerkhof

Alessandria

et al. 2023

Heliyon

Self Cite

View full text Add to dashboard Cite

“…On the other hand, five bacterial families were identified in the DC across both matrices (Table 1). While information is lacking for some regarding their presence in the intestinal microbiota or associated impacts, others are reported with some functions, such as Fusobacteriaceae, known for its association with systemic inflammation and frequent occurrence in obese microbiota [48], and Arcobacteraceae, linked to gastrointestinal symptoms like diarrhea, exhibit a diversity that includes nonpathogenic species capable of producing beneficial short-chain fatty acids (SCFA) and possessing a colonizing capacity [49]. Additionally, Tannerellaceae has been observed to play a role in breaking down flavonoids and benzene rings, with the growth of these bacterial groups stimulated in the presence of such compounds [50].…”

Section: Taxonomic Analysismentioning

confidence: 99%

The Effect of Opuntia ficus Mucilage Pectin and Citrus aurantium Extract Added to a Food Matrix on the Gut Microbiota of Lean Humans and Humans with Obesity

Estrada-Sierra,

Gonzalez-Avila,

Urias-Silvas

et al. 2024

Foods

View full text Add to dashboard Cite

Experimental studies have provided evidence that physicochemical interactions in the food matrix can modify the biologically beneficial effects of bioactive compounds, including their effect on gut microbiota. This work aimed to evaluate the effect of a food gel matrix with Opuntia ficus cladodes mucilage pectin and Citrus Aurantium extract on the growth of four beneficial gut bacteria obtained from the fecal microbiota of people who are lean or who have obesity after digestion in the upper digestive system. To accomplish this, a base formulation of Opuntia ficus cladodes mucilage with or without C. aurantium extract was submitted to an ex vivo fecal fermentation in an automatic and robotic intestinal system. The changes in the intestinal microbiota were determined by means of plate culture and 16S sequencing, while short-chain fatty acids (SCFA) produced in the colon were determined via gas chromatography. In the presence of the extract in formulation, greater growth of Bifidobacterium spp. (+1.6 Log10 Colonic Forming Unit, UFC) and Lactobacillus spp. (+2 Log10 UFC) in the microbiota of lean people was observed. Only the growth in Salmonella spp. (−1 Log10 UFC) from both microbiota was affected in the presence of the extract, which decreased in the ascending colon. SCFA was mainly produced by the microbiota of people who were lean rather than those who had obesity in the presence of the extract, particularly in the ascending colon. The effect of sour orange extract seems to depend on the origin of the microbiota, whether in people who have obesity (25 mM/L) or are lean (39 mM/L).

show abstract

“…The cell model preparation and colonization tests were performed in three biological replicates following a protocol used to study Arcobacter butzleri with some modifications [42,43]. A human colon carcinoma cell line HT29-MTX-E12 (12040401; ECACC) was cultured in Dulbecco's Modified Eagle Medium (DMEM, 6429; Sigma-Aldrich, St. Louis, MO, USA) with 10% v/v FBS (F7524; Sigma-Aldrich) and EmbryoMax penicillinstreptomycin (TMS-AB2-C; Sigma-Aldrich).…”

Section: Evaluation Of a Altamirensis Colonization On Human Cellsmentioning

confidence: 99%

“…In the AAI genome, the genes tonB and exbB, exbD, and tolB (TonB-related functions) are present. The protein TonB has been associated with virulence functions and host colonization in Gram-negative pathogens, like Escherichia coli and A. butzleri [43,50]. Even if the detection of other virulence genes was not possible, similarly to other A. altamirensis [17], the presence of tonB and related genes agrees with its in vitro colonization ability.…”

Section: Gene Enrichment and Virulence-antibiotic Resistance Gene Traitsmentioning

confidence: 99%

Aureimonas altamirensis: First Isolation from a Chicken Slaughterhouse in Italy Followed by Genotype and Phenotype Evaluations

Buzzanca,

Chiarini,

Mania

et al. 2023

Microbiology Research

Self Cite

View full text Add to dashboard Cite

The presence of foodborne pathogens in meat is linked to several contamination sources, and the slaughterhouse environment represents a relevant reservoir of contamination. Aureimonas altamirensis is a Gram-negative bacteria associated with different isolation sources, including human clinical cases. This study aims to identify and characterize an A. altamirensis isolate from chicken guts collected in an Italian slaughterhouse. The study approach includes whole-genome analysis jointly with phenotypical tests. Whole-genome sequencing (WGS) confirms the initial MALDI-TOF MS identification, finding putative virulence and biofilm-related genes. Moreover, the gene class evaluation reveals that the numerically largest gene category in the A. altamirensis genome is related to amino acid metabolism and transport. The analyses performed on a human gut mucus-producing cell line (HT29-MTX-E12) demonstrated the ability of A. altamirensis to colonize the host cell layer. Moreover, the antibiotic resistance test showed a high resistance of A. altamirensis to gentamicin (MIC 0.5 mg/L). The detection of a potential pathogenic and antibiotic-resistant A. altamirensis strain isolated from a slaughterhouse underlines the necessity of active surveillance studies focused on this species and the need for further studies about A. altamirensis in foods.

show abstract

Transcriptome Analysis of Arcobacter butzleri Infection in a Mucus-Producing Human Intestinal In Vitro Model

Cited by 6 publications

References 70 publications

Arcobacteraceae comparative genome analysis demonstrates genome heterogeneity and reduction in species isolated from animals and associated with human illness

Arcobacteraceae comparative genome analysis demonstrates genome heterogeneity and reduction in species isolated from animals and associated with human illness

The Effect of Opuntia ficus Mucilage Pectin and Citrus aurantium Extract Added to a Food Matrix on the Gut Microbiota of Lean Humans and Humans with Obesity

Aureimonas altamirensis: First Isolation from a Chicken Slaughterhouse in Italy Followed by Genotype and Phenotype Evaluations

Contact Info

Product

Resources

About