Metabolic engineering for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose and propionic acid in recombinant Escherichia coli

Meng, Dong; Miao, Changfeng; Liu, Yuling; Wang, Fang; Chen, Lu; Huang, Zida; Fan, Xiangyu; Gu, Pengfei; Li, Qiang

doi:10.1016/j.biortech.2022.126786

Cited by 14 publications

(4 citation statements)

References 43 publications

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“… 18 Furthermore, it is easily processed, allowing the development of different morphology matrices, as dense and porous films, fibers, 3D scaffolds, and microspheres, relying on different processing techniques. 16 The ability to be processed under different morphologies makes PHBV suitable to be tailored for specific microenvironments and applications, such as drug delivery, 19 , 20 tissue engineering, 16 , 21 antibacterial substrates, 22 and packaging, 23 among others. Porous matrices have been required to mimic porous structures in the human body as the cancellous bone morphology for example.…”

Section: Introductionmentioning

confidence: 99%

Piezoelectric and Magnetically Responsive Biodegradable Composites with Tailored Porous Morphology for Biotechnological Applications

Marques‐Almeida

Correia

Martín

et al. 2022

ACS Appl. Polym. Mater.

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The biomedical area in the scope of tissue regeneration pursues the development of advanced materials that can target biomimetic approaches and, ideally, have an active role in the environment they are placed in. This active role can be related to or driven by morphological, mechanical, electrical, or magnetic stimuli, among others. This work reports on the development of active biomaterials based on poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid), PHBV, a piezoelectric and biodegradable polymer, for tissue regeneration application by tailoring its morphology and functional response. PHBV films with different porosities were obtained using the solvent casting method, resorting to high-boiling-point solvents, as N,N-dimethylformamide (DMF) and dimethylsulfoxide (DMSO), and the combination of chloroform (CF) and DMF for polymer dissolution. Furthermore, magnetoelectric biomaterials were obtained through the combination of the piezoelectric PHBV with magnetostrictive iron oxide (Fe 3 O 4 ) nanoparticles. Independently of the morphology or filler content, all biomaterials proved to be suitable for biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Piezoelectric and Magnetically Responsive Biodegradable Composites with Tailored Porous Morphology for Biotechnological Applications

Marques‐Almeida

Correia

Martín

et al. 2022

ACS Appl. Polym. Mater.

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“…More genes associated with PHA synthesis had since been introduced into Escherichia coli for PHA synthesis. [73][74][75][76] Undoubtedly, the stable and strong expression of genes associated with PHA synthesis is very important to lead the substrate to directly synthesize PHA, thereby boosting the content of PHB accumulated in cells, in which the PhaC occupies the most important position. In order to enhance the activity of PhaC and change the substrate specificity, researchers have done a lot of works on the editing of PhaC.…”

Section: Recombinant Engineering Bacteria Fermentation Methodsmentioning

confidence: 99%

“…As expected, the resulting recombinant Escherichia coli presented an enhanced PHA synthesis capability. More genes associated with PHA synthesis had since been introduced into Escherichia coli for PHA synthesis 73–76 …”

Section: Biological Production Of Phamentioning

confidence: 99%

Current status and future perspectives on the biological production of polyhydroxyalkanoates

Che

Jin

Zhou

et al. 2023

Asia-Pacific J Chem Eng

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Nowadays, the environmental pollution caused by conventional petroleum‐based plastics is one of the most serious problems facing mankind, and it is of great significance to look for alternative plastics with biodegradable feature. Notably, the bioplastics based on polyhydroxyalkanoates (PHA) have been a focus on research and development during the past several decades. In this review, the recent advances on the biological production of PHA were summarized, mainly including biosynthetic pathways and methods, followed by the extraction processes. Furthermore, the current status on PHA production using industrial waste streams was also discussed. For the moment, the relative higher production cost hindered the large‐scale biological production of PHA. Therefore, some insightful strategies were presented about the future research and scale production on biological production of PHA.

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“…For this purpose, the addition of precursors can be combined with engineered strains, as well as other strategies, to obtain optimal results. phaCp and phaABp genes from Propylenella binnzhouense have been cloned in E. coli to produce PHBV, improving its synthesis activity and polymer performance at higher processing temperatures (Meng et al, 2022). Rhodoligotrophos defluvii was proved to be able to synthesize PHBV in the presence of propionate through the successful construction of a PHA synthesis pathway in recombinant E. coli (Miao et al, 2021).…”

Section: Operational Conditions For Biological Phbv Synthesismentioning

confidence: 99%

PHBV cycle of life using waste as a starting point: from production to recyclability

García-Chumillas,

Guerrero-Murcia,

Nicolás-Liza

et al. 2024

Front. Mater.

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Global concern about plastic pollution is forcing new policies and modifications of human consumption as well as promoting new research lines aiming at the replacement of non-degradable plastics with other polymers more environmentally friendly. Addressing food waste and promoting circular economy strategies, among other approaches, are crucial in reducing environmental impacts and fostering sustainability in several sectors like the agri-food industry. The European Union’s Circular Economy Action Plan is a significant initiative in this direction. Biotechnological processes, especially the valorisation of agri-food waste to produce highly marketed biomolecules like poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) using microorganisms as cellular factories, offer promising avenues for achieving these goals. PHBV is a biodegradable polymer firstly characterised as an isolated biopolymer from bacterial biomass. This biopolymer shows interesting physicochemical properties making possible immense potential in various applications due to its biocompatibility and sustainability, thus revealing it as a good candidate to replace plastics produced by chemical synthesis from petroleum (which are highly recalcitrant and consequently pollutants). This review critically analyses the PHBV synthesis and end-of-life scenarios from their synthesis using chemical and biological pathways, through the forms of biotechnological operation and production, to the forms described until the moment of recycling.

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Metabolic engineering for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose and propionic acid in recombinant Escherichia coli

Cited by 14 publications

References 43 publications

Piezoelectric and Magnetically Responsive Biodegradable Composites with Tailored Porous Morphology for Biotechnological Applications

Piezoelectric and Magnetically Responsive Biodegradable Composites with Tailored Porous Morphology for Biotechnological Applications

Current status and future perspectives on the biological production of polyhydroxyalkanoates

PHBV cycle of life using waste as a starting point: from production to recyclability

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