Metabolic engineering Corynebacterium glutamicum to produce triacylglycerols

Dynamic decoupling of biomass and wax ester biosynthesis in Acinetobacter baylyi by an autonomously regulated switch

“…As an example of a more advanced approach, Xu et al (2017) established a semi-continuous culture system with model-aided bioprocess optimization and cell recycling, which allowed efficient TAG production and high productivities even with dilute acetate feed in Yarrowia lipolytica . While also metabolic engineering strategies have been employed for improving TAG ( Runguphan and Keasling, 2014 , Plassmeier et al, 2016 , Tai and Stephanopoulos, 2013 , Santala et al, 2011 ) and WE synthesis ( Lehtinen et al, 2018a , Santala et al, 2014 , Wenning et al, 2017 ) in microbes, efficient overproduction of acetyl-CoA coupled with dynamic resource distribution between biomass and storage lipid synthesis remains a challenge.…”

Engineering a novel biosynthetic pathway in Escherichia coli for the production of caffeine

“…Many bio-based products have been successfully synthesized from renewable resources by heterologous microorganisms, including biodiesel, aromatic compounds, and amino acids. [10][11][12][13][14][15] These examples demonstrated that the microbial platform is economical and environmentally friendly approach for the production of plantderived compounds on an industrial scale. Consequently, synthesis of caffeine by metabolic engineering of microbes is of great scientic and commercial interest.…”

Optimizing recombineering in Corynebacterium glutamicum

“…Over the past 60 years, Corynebacterium glutamicum has been well known as an excellent producer of ʟ‐amino acids (Becker & Wittmann, 2012). Recent advances in metabolic and synthetic biology have expanded the portfolio of chemicals that can be produced from C. glutamicum , but it is still difficult to synthesize these compounds at an industrially relevant scale (S. Y. Kim et al, 2015; C. Li et al, 2020; L. Liu et al, 2013; Otten et al, 2015; Plassmeier et al, 2016; Woo & Park, 2014; Xu et al, 2019; Zha et al, 2018). Due to the increasing worldwide demand of amino acids and other novel compounds, there is a pressing need for rapid improvement in strain engineering and production yields of chemical compounds in C. glutamicum (Wendisch, 2007).…”

“…Due to the increasing worldwide demand of amino acids and other novel compounds, there is a pressing need for rapid improvement in strain engineering and production yields of chemical compounds in C. glutamicum (Wendisch, 2007). To construct novel metabolic pathways and redirect carbon flux, new tools are required that are optimized for C. glutamicum and improve the speed and efficiency of scarless genomic deletions, insertions, and mutations (Plassmeier et al, 2016; Woo & Park, 2014).…”