Metabolic effects of dinoseb, diazinon and esfenvalerate in eyed eggs and alevins of Chinook salmon (Oncorhynchus tshawytscha) determined by 1H NMR metabolomics

Fish & Shellfish Immunology

et al. 2017

a b s t r a c tSeawater salinity is one of the most important changeable environmental factors influencing the behavior, survival, growth and production of marine organisms. In this work, metabolite and gene expression profiles were used to elucidate the biological effects of reduced salinities in juvenile flounder Paralichthys olivaceus. Metabolic profiling indicated that both reduced salinities (23.3‰ and 15.6‰) enhanced proteolysis and disturbed osmotic regulation and energy metabolism in juvenile flounder P. olivaceus. Furthermore, the low salinity (15.6‰) enhanced anaerobic metabolism indicated by the elevated lactate in flounder tissue extracts. Gene expression profiles exhibited that reduced salinities could induce immune stress and oxidative stress and disturb energy metabolism in juvenile flounder P. olivaceus. In addition, reduced salinities might promote the growth and gonadal differentiation in juvenile flounder P. olivaceus.

Section: Resultssupporting

confidence: 85%

Metabolite and gene expression responses in juvenile flounder Paralichthys olivaceus exposed to reduced salinities

Fish & Shellfish Immunology

et al. 2017

“…High resolution proton nuclear magnetic resonance (HR-1 H NMR) spectroscopy is particularly suitable for the measurement of a large number of metabolites in biological samples because it is rapid, non-invasive and is rich in structural and quantitative information (Brindle et al, 2002;Lindon et al, 2000). NMRbased metabolomics has been effi ciently used in several recent studies in marine environmental toxicology (Viant et al, 2001;Viant et al, 2006aViant et al, , 2006bJones et al, 2008;Williams et al, 2009;Gordon and Leggat, 2010;Lannig et al, 2010;Santos et al, 2010;Tikunov et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…In the post-genomic era, metabolomics represents a systems biology approach that has been successfully applied in multiple studies, including drug toxicity, and disease diagnosis toxicology (Brindle et al, 2002;Bundy et al, 2004;Viant et al, 2006aViant et al, , 2006bWu et al, 2005aWu et al, , 2005b. Metabolomics focuses on all of the low molecular weight (<1 000 Da) metabolites, which are the end-products of metabolism, and which represent the functional responses of biological systems (e.g., in cells, tissues, urine, or plasma) (Lindon et al, 2000;Viant et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

Wu²,

Chin. J. Ocean. Limnol.

et al. 2013

Ruditapes philippinarum , a clam that thrives in intertidal zones of various salinities, is a useful biomonitor to marine contaminants. We investigated the infl uence of dilution to 75% and 50% of normal seawater salinity (31.1) on the responses of the digestive gland of R . philippinarum to arsenic exposure (20 μg/L), using nuclear magnetic resonance (NMR)-based metabolomics. After acute arsenic exposure for 48 h, salinity-dependent differential metabolic responses were detected. In normal seawater, arsenic exposure increased the concentrations of branched-chain amino acids, and of threonine, proline, phosphocholine and adenosine, and it decreased the levels of alanine, hypotaurine, glucose, glycogen and ATP in the digestive glands. Differential changes in metabolic biomarkers observed at lower salinity (~23.3) included elevation of succinate, taurine and ATP, and depletion of branched-chain amino acids, threonine and glutamine. Unique effects of arsenic at the lowest salinity (~15.6) included down-regulation of glutamate, succinate and ADP, and up-regulation of phosphocholine. We conclude that salinity infl uences the metabolic responses of this clam to arsenic.

“…Proton NMR ( 1 H-NMR) spectroscopy-based metabolomics is a post-genomic approach that combines the high throughput meta-bolic profiling capabilities of 1 H-NMR with pattern recognition (PR) techniques to identify the metabolic differences (metabolic biomarkers) between samples [14]. Such study of the low molecular weight (<1000 Da) metabolite profiles has been widely used in drug toxicity, disease diagnosis, functional genomics, and environmental toxicology [18,19]. It is well established and developed that NMRbased metabolomics can provide valuable biochemical information on the perturbations that are induced by both endogenous (e.g., inborn diseases) and exogenous factors (e.g., environmental contaminants), through the analysis of biofluids and tissues [20,21].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR‐Based Metabolomics

Zhang

You

et al. 2011

CLEAN Soil Air Water

Research Article Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based MetabolomicsThere are mainly distributed three pedigrees (White, Liangdao Red, and Zebra) of Manila clam Ruditapes philippinarum in Yantai population along the Bohai marine and coast. However, the biological differences to environmental stressors have been ignored in toxicology studies, which could lead to the distortion of biological interpretations of toxicological effects induced by environmental contaminants. In this study, we applied a system biology approach, metabolomics to compare the metabolic profiles in digestive gland from three pedigrees of clam and characterize and compare the metabolic responses induced by mercury in clam digestive gland tissues to determine a sensitive pedigree of clam as a preferable bioindicator for metal pollution monitoring and toxicology research. The most abundant metabolites, respectively, included branched-chain amino acids, alanine, and arginine in White samples, glutamate, dimethylglycine, and glycine in Zebra clams and acetylcholine, betaine, glucose, and glycogen in Liangdao Red clams. After 48 h exposure of 20 mg L À1 Hg 2þ , the metabolic profiles from the three pedigrees of clams showed differentially significant changes in alanine, glutamate, succinate, taurine, hypotaurine, glycine, arginine, glucose, etc. Our findings indicate the toxicological effects of mercury exposure in Manila clams including the neurotoxicity, disturbances in energetic metabolisms and osmoregulation in the digestive glands and suggest that Liangdao Red pedigree of clam could be a preferable bioindicator for the metal pollution monitoring based on the more sensitive classes of metabolic changes from digestive glands compared with other two (White and Zebra) pedigrees of clams.