Metabolic effects of an experimental histidinemia

Woodworth, Mary E.; Baldridge, Robert C.

doi:10.1016/0006-2944(70)90071-2

Cited by 12 publications

(9 citation statements)

References 16 publications

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Section: Discussionmentioning

confidence: 99%

“…Rats fed the 5-percent histidine diet showed considerably higher levels of histidine in the blood, an 11-to 16-fold increase (23) (table III), compared with that ob served in human histidinemia. The histidine-fed rats showed a 20-percent de crease (23) (table II) in hepatic histidase activity, but an increased excretion of glutamate, produced by the deamination pathway (23). In this study, we showed that rats treated with nitromethane had an increase in plasma histidine concen tration of almost the same magnitude as the human histidinemics and a reduc tion of in vivo CO2 production from histidine, indicating a depression of the deamination pathway.…”

Section: Discussionmentioning

confidence: 99%

“…However, histidinemia produced in this way did not actually mimic the genetic disorder because the histidase activity was rela tively unaffected and glutamate production via the deamination pathway was even elevated in the high histidine-fed rats (23). Givot and Abeles (5) and Givot et al (6) recently found that nitromethane was able to bind covalently to the electrophilic center of the active site of histidase and thus inactivated its activity.…”

Section: Introductionmentioning

confidence: 99%

“…Woodworth and Baldridge (23) reported an experimental procedure to in duce a continuous histidinemia in rats; this consisted of feeding a diet containing 20 % casein and 5 % ¿-histidine. However, histidinemia produced in this way did not actually mimic the genetic disorder because the histidase activity was rela tively unaffected and glutamate production via the deamination pathway was even elevated in the high histidine-fed rats (23).…”

Section: Introductionmentioning

confidence: 99%

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Histidinemia Produced in the Rat by Treatment with Nitromethane

Lee

Wang²

1975

Ann Nutr Metab

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Metabolic effects of in vivo administration of nitromethane, a histidase inhibitor, were studied in the rat. Histidinemia produced by feeding a 5-percent histidine diet to the rat was included for comparison. Rats injected subcutaneously with nitromethane every other day appeared to be histidinemic, with a plasma histidine level of 43 µmol/100 ml (control 9.2 µmol/100 ml) after 12 days of treatment. Histidine concentrations in the brain, liver, kidney, and urine of the nitromethane-treated rats were also increased. Liver histidase activity was decreased about 75 % after nitromethane administration, and the rate of in vivo CO₂ production from histidine was also depressed. No abnormal change in liver and kidney sizes was observed in these animals. Rats fed the 5-percent histidine diet showed markedly higher plasma histidine concentrations (99 µmol/100 ml) than the nitromethane-treated rats. The magnitude of increase in plasma histidine in the histidine-fed rats was considerably greater than that observed in humans with histidinemia. Furthermore, the liver histidase activity was decreased only by about 20 %, and a significant increase in liver and kidney sizes was noted in the histidine-fed animals. These findings indicate that histidinemia produced by nitromethane administration is a better model for studies of the genetic disorder than histidinemia induced by high levels of histidine in the diet.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Histidinemia Produced in the Rat by Treatment with Nitromethane

Lee

Wang²

1975

Ann Nutr Metab

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“…Using the spectrophotometric assays [8] [16]. Using the same experimental method histidine-pyruvate aminotrans ferase but not histidine ammonia-lyase activity was identified in rat brain tissue [20] while histidine ammonia-lyase has also been reported to be present in the liver of cats, rabbits and dogs but absent in the pancreas, kidney, duodenum, stomach, spleen and thyroid of the pig and in horseheart [4].…”

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confidence: 99%