Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives

Noda, Shinshi; Shirai, Toshiaki; Oyama, Sachiko; Kondo, Akihiko

doi:10.1016/j.ymben.2015.11.007

Cited by 102 publications

(108 citation statements)

References 53 publications

Supporting

Mentioning

106

Contrasting

Order By: Relevance

“…13,14 To overcome the toxicity of vanillin, a gene encoding family 1 UDP-glycosyltransferase from Arabidopsis thaliana was expressed in Schizosaccharomyces pombe and the engineered enzyme catalyzed the conversion of vanillin to vanillin -D-glucoside, which is not toxic to the yeast cells and thus may be accumulated in larger amounts. 4,6 There are four different pathways for biosynthesis of MA using chorismate as precursor from renewable feedstocks, but the titers reported are lower than that of produced via AroZ-AroY pathway ( Fig. 4,6 There are four different pathways for biosynthesis of MA using chorismate as precursor from renewable feedstocks, but the titers reported are lower than that of produced via AroZ-AroY pathway ( Fig.…”

Section: Production Of Dhs Derivativesmentioning

confidence: 99%

“…4,6 There are four different pathways for biosynthesis of MA using chorismate as precursor from renewable feedstocks, but the titers reported are lower than that of produced via AroZ-AroY pathway ( Fig. 6 p-Hydroxybenzoic acid and anthranilic acid, two intermediates of MA biosynthetic pathways, can be halogenated by corresponding flavin-dependent halogenases (Fig. Production of chorismate and its derivatives will release pyruvate that causes the reduced yields from renewable feedstocks.…”

Section: Production Of Dhs Derivativesmentioning

confidence: 99%

“…Then, chorismate is converted into phenylpyruvate (PP) or 4-hydroxyphenylpyruvate (4HPP) via prephenate by Phe-Tyr branch that is catalyzed by chorismate mutase/prephenate dehydratase (PheA) or the chorismate mutase/prephenate dehydrogenase (TyrA) complex. 1,3,4,6 Microorganisms, those with defined genetic background and effective genetic toolkits such as Escherichia coli, Saccharomyces cerevisiae and Corynebacterium glutamicum, are usually used as host strains to produce industrially valuable aromatic chemicals through metabolic engineering and synthetic biology approaches. Lastly, Phe, Tyr and Trp are synthesized via their own biosynthetic pathways using phenylpyruvate, 4-hydroxyphenylpyruvate or anthranilate as precursors, respectively (Fig.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Expanding the repertoire of aromatic chemicals by microbial production

Song

Chen

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

Microbial production of aromatic chemicals would greatly contribute to solving the problems with fossil resource supply and environmentally sustainable development. Engineering and extending the shikimate/aromatic amino acid biosynthetic pathways are important routes for microbial production of various aromatic chemicals. With advances in metabolic engineering and synthetic biology, we can broaden the product spectrum and obtain several valuable and novel aromatic chemicals from renewable feedstocks. Here, in this review, the latest research progress on microbial production of various aromatic chemicals, and recent metabolic engineering and synthetic biology strategies targeting the central carbon metabolism, the shikimate and aromatic amino acid biosynthetic pathways are summarized and discussed. This work aims to provide some valuable tips for the construction of cost‐effective engineered strains for producing various aromatic chemicals. © 2018 Society of Chemical Industry

show abstract

Section: Production Of Dhs Derivativesmentioning

confidence: 99%

Section: Production Of Dhs Derivativesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expanding the repertoire of aromatic chemicals by microbial production

Song

Chen

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…Other intermediates within the shikimate pathway can redirected to the synthesis of phenol and cis , cis ‐muconic acid. The formation of the key metabolites PEP and E4P has been manipulated in order to increase the overall flux through the shikimate route, and, in a recent example, these manipulations lead to a significant increase in salicylic acid formation (Noda et al ., ). Another strategy adopted for this purpose has been to relieve the feedback regulation that occurs in one of the first enzymes of the pathway, AroF.…”

Section: Bacterial Species Adopted As a Chassis: From Historical Exammentioning

confidence: 99%

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Calero

Nikel

2018

Microbial Biotechnology

228

184

View full text Add to dashboard Cite

The last few years have witnessed an unprecedented increase in the number of novel bacterial species that hold potential to be used for metabolic engineering. Historically, however, only a handful of bacteria have attained the acceptance and widespread use that are needed to fulfil the needs of industrial bioproduction - and only for the synthesis of very few, structurally simple compounds. One of the reasons for this unfortunate circumstance has been the dearth of tools for targeted genome engineering of bacterial chassis, and, nowadays, synthetic biology is significantly helping to bridge such knowledge gap. Against this background, in this review, we discuss the state of the art in the rational design and construction of robust bacterial chassis for metabolic engineering, presenting key examples of bacterial species that have secured a place in industrial bioproduction. The emergence of novel bacterial chassis is also considered at the light of the unique properties of their physiology and metabolism, and the practical applications in which they are expected to outperform other microbial platforms. Emerging opportunities, essential strategies to enable successful development of industrial phenotypes, and major challenges in the field of bacterial chassis development are also discussed, outlining the solutions that contemporary synthetic biology-guided metabolic engineering offers to tackle these issues.

show abstract

“…Chorismate, for example, is a versatile endogenous intermediate with the potential to be converted to various aromatic end products. Following extensive host engineering to enhance endogenous chorismate production, Noda and co‐workers introduced seven unique downstream pathway modules to produce seven unique chorismate‐derived products, including salicylate, phenol, 3‐hydroxybenzoate, and cis,cis ‐muconic acid, among others . This approach was further exploited by Shen et al ., in this case to convert chorismate to either salicyl alcohol (precursor to the anti‐inflammatory, salicin) or gentisyl alcohol (an antioxidant and antibiotic); both through salicylate as a common intermediate …”

Section: Modular Engineering Strategies For Optimizing Pathway Flux Amentioning

confidence: 99%

Emerging tools, enabling technologies, and future opportunities for the bioproduction of aromatic chemicals

Machas

Kurgan

Jha

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

Aromatic compounds, which are traditionally derived from petroleum feedstocks, represent a diverse class of molecules with a wide range of industrial and commercial applications. Significant progress has been made to alternatively and sustainably produce many aromatics from renewable substrates using microbial biocatalysts. While the construction of both natural and non‐natural pathways has expanded the number and diversity of aromatic bioproducts, pathway modularization in both single‐ and multi‐strain systems continues to support the enhancement of key production metrics towards economically‐viable levels. Emerging tools for implementing more precise metabolic control (e.g. CRISPRi, sRNA) as well as the engineering of novel high‐throughput screening platforms utilizing in vivo aromatic biosensors, meanwhile, continue to facilitate further optimization of both pathways and hosts. While product toxicity persists as a key challenge limiting the production of many aromatics, various successful strategies have been demonstrated towards improving tolerance, including via membrane and efflux pump engineering as well as by exploiting alternative production hosts. Finally, as a further step towards sustainable and economical aromatic bioproduction, non‐model substrates including lignin‐derived compounds continue to emerge as viable feedstocks. This review highlights recent and notable achievements related to such efforts while offering future outlooks towards engineering microbial cell factories for aromatic production. © 2018 Society of Chemical Industry

show abstract

Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives

Cited by 102 publications

References 53 publications

Expanding the repertoire of aromatic chemicals by microbial production

Expanding the repertoire of aromatic chemicals by microbial production

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Emerging tools, enabling technologies, and future opportunities for the bioproduction of aromatic chemicals

Contact Info

Product

Resources

About