Metabolic changes in Japanese medaka (Oryzias latipes) during embryogenesis and hypoxia as determined by in vivo 31P NMR

Pincetich, Christopher A.; Viant, Mark R.; Hinton, David E.; Tjeerdema, Ronald S.

doi:10.1016/j.cca.2005.01.010

Cited by 34 publications

(33 citation statements)

References 31 publications

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“…TCE appears to have exceeded this buffering capability since ATP levels were also significantly depleted. A similar depletion of both metabolites was observed during a 1-h hypoxic challenge of 7-day-old (stage 37) medaka embryos (Pincetich et al, 2005), which is consistent with a hypoxia-induced reduction or cessation of aerobic metabolism. The decrease in PCr concentration in TCE-exposed eggs clearly indicates that it is inflicting an energetic cost on the developing medaka embryo.…”

Section: Metabolic Biomarkers Of Tce Toxicitysupporting

confidence: 80%

NMR-derived developmental metabolic trajectories: an approach for visualizing the toxic actions of trichloroethylene during embryogenesis

et al. 2005

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Fish embryo toxicity tests for chemical risk assessment have traditionally been based upon non-specific endpoints including morphological abnormalities, hatching success, and mortality. Here we extend the application of 1 H NMR-based metabolomics in environmental toxicology by adding a suite of metabolic endpoints to the Japanese medaka (Oryzias latipes) embryo assay, with the goal to provide more sensitive, specific and unbiased biomarkers of toxicity. Medaka were exposed throughout embryogenesis to five concentrations of trichloroethylene (TCE; 0, 8.76, 21.9, 43.8, 87.6, 175 mg/L) and the relative sensitivities of the traditional and metabolomic endpoints compared. While the no-observable-adverse-effect-level for hatching success, the most sensitive traditional indicator, was 164 mg/L TCE, metabolic perturbations were detected at all exposure concentrations. Principal components analysis (PCA) highlighted a dose-response relationship between the NMR spectra of medaka extracts. In addition, 12 metabolites that exhibited highly significant dose-response relationships were identified, which indicated an energetic cost to TCE exposure. Next, embryos were exposed to 0, 0.88, 8.76 mg/L TCE and sampled on each of the 8 days of development. Projections of 66 twodimensional J-resolved NMR spectra were obtained, and PCA revealed developmental metabolic trajectories that characterized the basal and TCE-perturbed changes in the entire NMR-visible metabolome throughout embryogenesis. Although no significant increases in mortality, gross deformity or developmental retardation were observed relative to the control group, TCE-induced metabolic perturbations were observed on day 8. In conclusion, these results support the continued development of NMR-based metabolomics as a rapid and reproducible tool for biomarker discovery and environmental risk assessment.

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Section: Metabolic Biomarkers Of Tce Toxicitysupporting

confidence: 80%

NMR-derived developmental metabolic trajectories: an approach for visualizing the toxic actions of trichloroethylene during embryogenesis

et al. 2005

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“…Increases in succinate and valine and decreases in leucine and isoleucine have been observed in 1 H NMR partial ecometabolomic studies of polar metabolites in the mussels Mytilus edulis and Mytlus galloprovincialis grown at different hypoxia levels (Hines et al 2007;Tuffnail et al 2009) ( Table 1). The fish Oryzias latipes has shown increases of soluble phosphates and decreases in ATP and phosphodiesters in response to hypoxia in a PEM of phosphorylated soluble metabolites by 31 P NMR (Pincetich et al 2005 (Li et al 2006). Thus, the responses of the most polar metabolite groups were different depending on the genotypes.…”

Section: Hypoxiamentioning

confidence: 99%

Ecological metabolomics: overview of current developments and future challenges

2011

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Ecometabolomics, which aims to analyze the metabolome, the total number of metabolites and its shifts in response to environmental changes, is gaining importance in ecological studies because of the increasing use of new technical advances, such as modern HNMR spectrometers and GC-MS coupled to bioinformatic advances. We review here the state of the art and the perspectives of ecometabolomics. The studies available demonstrate ecometabolomic techniques have great sensitivity in detecting the phenotypic mechanisms and key molecules underlying organism responses to abiotic environmental changes to biotic interactions. But such studies are still scarce, and in most cases they are limited to the direct effects of a single abiotic factor or of biotic interactions between two trophic levels under controlled conditions. Several exciting challenges remain to be achieved through the use of ecometabolomics in field conditions, involving more than two trophic levels, or combining the effects of abiotic gradients with intra-and inter-specific relationships. The coupling of ecometabolomic studies with genomics, transcriptomics, ecosystem stoichiometry, community biology and biogeochemistry may provide a further step forward in many areas of ecological sciences, including stress responses, species lifestyle, life history variation, population structure, trophic interaction, nutrient cycling, ecological niche and global change.

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“…For the latter scenario, stage 32 was chosen because it coincided with onset of formation of the swim bladder and initiation of blood circulation (Iwamatsu, 2004). In addition, we have shown several metabolic changes to be influenced by hypoxia in medaka at or near this developmental stage (Pincetich et al, 2005). All scenarios were run with 5 embryos in each of 3 replicates, and exposures were performed in a Heraeus Heracell 150 Tri-Gas Cell Culture Incubator (Thermo Scientific, Waltham, MA) with oxygen adjustment by nitrogen injection.…”

Section: Methodsmentioning

confidence: 97%

“…During summer, dissolved oxygen (DO) concentrations in tidal creeks can fall below 2 mg/L from several minutes to several hours daily (Tyler et al, 2009; Yang et al, 2013). Effects arising from hypoxia include: growth and reproductive impairments; altered gene expression; altered energy metabolism; increased resistance of offspring to hypoxia; and disruption of sex hormones (Cheek, 2011; Cheung et al, 2014; Ho and Burggren, 2012; Lai et al, 2016; Pincetich et al, 2005; Shang et al, 2006; Wu et al, 2003; Yu et al, 2015; Yu et al, 2012; Yu et al, 2008). …”

Section: Introductionmentioning

confidence: 99%

Early life co-exposures to a real-world PAH mixture and hypoxia result in later life and next generation consequences in medaka (Oryzias latipes)

Chernick

Wang

et al. 2017

Aquatic Toxicology

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Acute effects of individual and complex mixtures of polycyclic aromatic hydrocarbons (PAHs) are well documented in vertebrate species. Hypoxia in fish reduces metabolic rate and reproduction. However, less is known about the later life consequences stemming from early-life exposure to PAHs or hypoxia, particularly their co-exposure. To address this, medaka (Oryzias latipes) embryos were exposed to a complex PAH mixture sediment extract from the Elizabeth River, VA (ERSE) at concentrations of 0.1, 0.5, or 1.0% or to one of three different hypoxia scenarios: continuous, nocturnal, or late stage embryogenesis hypoxia. Co-exposures with 0.1% ERSE and each of the hypoxia scenarios were conducted. Results included decreased survival with ERSE, hatching delays with hypoxia, and higher occurrences of deformities with each. The continuous hypoxia scenario caused the most significant changes in all endpoints. These early-life exposures altered later-life growth, impaired reproductive capacity, and reduced the quality of their offspring. ERSE alone resulted in a female-biased sex ratio while continuous or nocturnal hypoxia produced significantly greater numbers of males; and co-exposure produced an equal sex ratio. Exposure to a PAH mixture and hypoxia during early life stages has meaningful later-life and next generational consequences.

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Metabolic changes in Japanese medaka (Oryzias latipes) during embryogenesis and hypoxia as determined by in vivo 31P NMR

Cited by 34 publications

References 31 publications

NMR-derived developmental metabolic trajectories: an approach for visualizing the toxic actions of trichloroethylene during embryogenesis

NMR-derived developmental metabolic trajectories: an approach for visualizing the toxic actions of trichloroethylene during embryogenesis

Ecological metabolomics: overview of current developments and future challenges

Early life co-exposures to a real-world PAH mixture and hypoxia result in later life and next generation consequences in medaka (Oryzias latipes)

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