Metabolic changes in deafferented central neurons of an insect, Acheta domesticus. I. Effects upon amino acid uptake and incorporation

Meyer,; Edwards, JS

doi:10.1523/jneurosci.02-11-01651.1982

Cited by 7 publications

(9 citation statements)

References 27 publications

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“…These cells respond to removal of cereal sensory axon terminals during postembryonic development with substantial retardation ofgrowth of dendritic processes and spines and by alterations in physiological response properties. We have shown that these changes coincide with a marked decrease in protein synthetic capacity within the dendrites and somata of deafferented giant interneurons (Meyer and Edwards, 1982). These findings suggested that the presence of afferent terminals might serve to regulate transneuronally the level of protein metabolism within giant interneurons during postembryonic development.…”

mentioning

confidence: 63%

“…Procedures for rearing, maintaining, and deafferentating crickets by cercectomy are described in detail elsewhere (Edwards and Pallor, 1974;Meyer and Edwards, 1982;Palka and Edwards, 1974). Briefly, deafferentation was achieved by removing one or both of the paired cereal appendages ( Fig.…”

Section: Deafferentationmentioning

confidence: 99%

“…and protein content may be significant during chronic deafferentation (-30%; Meyer and Edwards, 1982) these changes are accounted for in measurements of specific activity of the binding, which are always adjusted for total ganglionic protein.…”

Section: Morphological Correlates Of Deaferentationmentioning

confidence: 99%

“…

Long-term deafferentation sustained throughout postembryonic development markedly reduces the densities of both muscarinic and nicotinic binding sites in the TG, short-term deafferentation of adult TG also leads to characteristic alterations in the properties of all three choline@ markers within several days.

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mentioning

confidence: 99%

“…Following the finding that cereal deafferentation of developing giant interneurons in the terminal abdominal ganglion (TG) of the cricket Ache& dome&us reduces TG protein metabolism within target interneuron dendrites and somata (Meyer and Edwards, 1982), it is now shown that deafferentation alters the turnover of three macromolecules associated with cholinergic transmission in the cereal sensory-giant interneuron pathway.…”

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confidence: 99%

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Metabolic changes in deafferented central neurons of an insect, Acheta domesticus. II. Effects on cholinergic binding sites and acetylcholinesterase

Meyer¹,

Reddy²,

Edwards³

1986

J. Neurosci.

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Following the finding that cereal deafferentation of developing giant interneurons in the terminal abdominal ganglion (TG) of the cricket Ache& dome&us reduces TG protein metabolism within target interneuron dendrites and somata (Meyer and Edwards, 1982), it is now shown that deafferentation alters the turnover of three macromolecules associated with cholinergic transmission in the cereal sensory-giant interneuron pathway.The labeled specific ligands 3H-quinuclidinyl benzilate and 1251-cY-bungarotoxin were used to assay populations of putative TG muscarinic and nicotinic cholinergic receptors, respectively, in control and deafferented groups of ganglia. The AChE activity of TG was also determined by assay and histochemical methods.Long-term deafferentation sustained throughout postembryonic development markedly reduces the densities of both muscarinic and nicotinic binding sites in the TG, short-term deafferentation of adult TG also leads to characteristic alterations in the properties of all three choline@ markers within several days. Rapid changes seen in adults thus correlate with ultrastructural data demonstrating loss of afferent terminals within hours of sensory appendage removal.We propose that peripheral innervation-dependent regulatory mechanisms operate in both the developing and adult insect nervous system. Such mechanisms may influence transsynaptitally the synthesis and turnover of specilic macromolecules, some of which may reside on the cell surface of insect central neurons that are part of the cereal sensory-giant intemeuron system.

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mentioning

confidence: 63%

Section: Deafferentationmentioning

confidence: 99%

Section: Morphological Correlates Of Deaferentationmentioning

confidence: 99%

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…”

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confidence: 99%

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Metabolic changes in deafferented central neurons of an insect, Acheta domesticus. II. Effects on cholinergic binding sites and acetylcholinesterase

Meyer¹,

Reddy²,

Edwards³

1986

J. Neurosci.

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Afferent influences on brain stem auditory nuclei of the chicken: Time course and specificity of dendritic atrophy following deafferentation

Deitch

Rubel

1984

J of Comparative Neurology

160

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The time course and specificity of the changes in dendritic morphology following deafferentation were examined in nucleus laminaris of young chickens. The dendrites of nucleus laminaris neurons are segregated into dorsal and ventral domains, which are innervated separately from the ipsilateral and contralateral nucleus magnocellularis, respectively. Transection of the crossed dorsal cochlear tract deafferents the ventral dendrites of nucleus laminaris bilaterally without interrupting the matching input to the dorsal dendrites. In 10-day-old chicks, atrophy of the ventral dendrites began immediately after transecting the tract; the ventral dendrites were 10% shorter by 1 hour and 16% shorter by 2 hours after deafferentation. The length of the ventral dendrites progressively decreased over the next 2 weeks, resulting in at least a 60% loss of ventral dendrite 16 days after surgery. The dorsal dendrites of the same cells, whose afferents remained intact, did not change in length during the time course of this study. However, 16 days after the lesion, spines appeared on the normally smooth dorsal and ventral dendrites. The time course of dendritic atrophy and its restriction to the deafferented postsynaptic surface are related to possible mechanisms by which afferents regulate and maintain their target neurons.

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Afferent influences on brain stem auditory nuclei of the chicken: Cessation of amino acid incorporation as an antecedent to age‐dependent transneuronal degeneration

Steward

Rubel

1985

J of Comparative Neurology

113

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Previous studies of the avian auditory system have revealed that removal of the peripheral receptor (the cochlea) leads to a transneuronal degeneration of auditory relay neurons in nucleus magnocellularis (NM) of the brain stem. An early manifestation of the degeneration which can be observed within 12 hours is a decrease of histochemical staining for RNA (Nissl staining); such a decrease could reflect an alteration in protein synthetic activity within the NM neurons. The present study evaluates this possibility by determining whether the cochlea removal led to an alteration incorporation of protein precursors in the target neurons which exhibit transneuronal degeneration and if so, how early the changes appeared. The cochlea was removed unilaterally in seventeen 10-day-old chicks and two 66-week-old mature chickens, and incorporation of protein precursors was evaluated in the neurons of NM at 0.5, 1.5, 3, 6, 12, and 24 hours following the cochlea removal. Each chick received an intravenous injection of 3H leucine, and was allowed to survive for 30 minutes after the injection of precursor. The brains were then prepared for autoradiography. The extent of incorporation by neurons in NM was determined by counting grains overlying each cell body and determining grain density/micrometers2 of neuron cross-sectional area. We found that auditory relay neurons whose synaptic inputs have been silenced exhibit dramatic decreases in protein synthesis within 30 minutes after removal of the cochlea; leucine incorporation was reduced by about 50%. In chicks sacrificed 3 to 24 hours after removal of the cochlea, some neurons (about 1/3) were entirely unlabeled despite heavy labeling of their neighbors and heavy labeling of all NM neurons on the opposite side of the brain. The remaining neurons exhibited about a 15% reduction in incorporation in comparison with the cells in the contralateral (control) NM. While the decreases in incorporation were apparent at all survival intervals, there was no consistent decrease in Nissl staining until 6 hours after cochlea removal. There were no changes in protein precursor incorporation following removal of the cochlea in adult birds, a result which is in keeping with the relative absence of transneuronal degeneration following removal of the cochlea at maturity. The results suggest a very rapid transneuronal regulation of protein metabolism within target neurons in young animals, perhaps by activity-related events.

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Metabolic changes in deafferented central neurons of an insect, Acheta domesticus. I. Effects upon amino acid uptake and incorporation

Cited by 7 publications

References 27 publications

Metabolic changes in deafferented central neurons of an insect, Acheta domesticus. II. Effects on cholinergic binding sites and acetylcholinesterase

Metabolic changes in deafferented central neurons of an insect, Acheta domesticus. II. Effects on cholinergic binding sites and acetylcholinesterase

Afferent influences on brain stem auditory nuclei of the chicken: Time course and specificity of dendritic atrophy following deafferentation

Afferent influences on brain stem auditory nuclei of the chicken: Cessation of amino acid incorporation as an antecedent to age‐dependent transneuronal degeneration

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