Metabolic and proteomic analysis of morel fruiting body (Morchella importuna)

Wang, Jinqiu; Xiao, Jing; Geng, Fang; Li, Xiang; Yu, Juan; Zhang, Yuqi; Chen, Yan; Liu, Dayu

doi:10.1016/j.jfca.2018.12.006

Cited by 52 publications

(36 citation statements)

References 32 publications

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“…The extracted TBS total protein samples (200 μg) were reduced with 5 mmol/L dithiothreitol at 56°C for 30 min, and then alkylated with 10 mmol/L of iodoacetamide for 15 min at room temperature in the dark. Subsequently, the TBS total protein was digested using trypsin (Sigma‐Aldrich) at 1:50 trypsin‐to‐protein mass ratio for the first digestion overnight and 1:100 trypsin‐to‐protein mass ratio for a second 4‐hr digestion (Geng et al, ; Wang et al, ). Three biological replicates were performed to ensure protein reproducibility.…”

Section: Methodsmentioning

confidence: 99%

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Analysis of tartary buckwheat ( Fagopyrum tataricum ) seed proteome using offline two‐dimensional liquid chromatography and tandem mass spectrometry

et al. 2019

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The whole seed of tartary buckwheat (Fagopyrum tataricum) is considered as a healthy and functional food, which is rich in kinds of flavonoids and with potential antioxidant effect. An in‐depth analysis of tartary buckwheat seed (TBS) proteome was performed using a shotgun proteomics strategy. Total protein of TBS was extracted and digested, then the peptides were separated by offline two‐dimensional liquid chromatography and identified by tandem mass spectrometry. Total of 3,363 high‐confidence proteins were identified from 13,730 matched peptides, in which, 2,499 proteins were annotated by the Gene Ontology (GO) analysis with 1,720 involved in “biological process,” 2,241 in “molecular function,” and 693 in “cellular components.” Based on the GO functional enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment results, buckwheat seed proteins were mostly enriched in metabolism of nucleic acid, respiration and energy metabolism, as well as synthesis and metabolism of protein. Practical applications This study characterized the tartary buckwheat seed proteome on a scale of 3,000+ proteins and provide important information and clues for future research, especially in the mechanism of seed germination, nutrient composition changes, and metabolite production seed germination and material metabolism.

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Section: Methodsmentioning

confidence: 99%

“…Peptides were then selected for MS/MS using a dynamically excluded time of 30 s, and the fragments were detected at a resolution of 17,500. Automatic gain control was set at 5E4 (Geng, Wang, Liu, Jin, & Ma, 2017;Geng et al, 2018;Wang et al, 2019).…”

Section: Lc-ms/msmentioning

confidence: 99%

Analysis of tartary buckwheat ( Fagopyrum tataricum ) seed proteome using offline two‐dimensional liquid chromatography and tandem mass spectrometry

et al. 2019

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“…Afterward, the samples were diluted by adding 100 mM triethylamine borane (TEAB) to achieve a urea concentration of less than 2 mol/L. Finally, samples were digested using trypsin (Sigma‐Aldrich) at a 1:50 trypsin‐to‐protein mass ratio for the first digestion overnight and at a 1:100 trypsin‐to‐protein mass ratio for the second 4 hr digestion (Wang, Xiao, Geng, et al, ; Wang, Xiao, Liu, et al, ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

In‐depth mapping of the proteome of Tibetan pig tenderloin ( longissimus dorsi ) using offline high‐pH reversed‐phase fractionation and LC‐MS/MS

Gao

Luo

et al. 2019

J Food Biochem

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In recent years, Tibetan pig breeding and meat processing have developed rapidly. However, the basic physiological and biochemical characteristics of Tibetan pork have not been systematically explored. The present study conducted a high‐throughput analysis of the tenderloin (longissimus dorsi) proteome of the Tibetan pigs and performed a functional annotation and bioinformatics analysis of the identified proteins. Based on offline two‐dimensional liquid chromatography fractionation and MS/MS identification, a total of 1,723 proteins were identified in the tenderloin of Tibetan pigs. Gene ontology analysis and pathway enrichment analysis revealed that the proteins involved in respiration (oxidative phosphorylation, glycolysis/gluconeogenesis, citric acid cycle, and pyruvate metabolism) and protein synthesis and metabolism (proteasome, amino acid biosynthesis, endoplasmic reticulum protein processing, and ribosomes) were significantly enriched, indicating that the energy production and protein metabolism are the most important physiological processes in Tibetan pig tenderloin. Practical applicationsThe in‐depth mapping of the tenderloin (longissimus dorsi) proteome of the Tibetan pigs gives a panoramic perspective at the protein molecular level and provides important information on the mechanisms of postmortem muscle physiology and meat quality formation. Furthermore, the development of Tibetan pork storage and processing technologies would also benefit from the characterization of the biochemical properties of Tibetan pork.

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“…Morchella (Morchella esculenta L.) is commonly known as sheep belly, lamb belly mushroom, is recognized as a valuable edible mushroom in the world [1]. It is rich in a variety of biologically active ingredients, and its texture is crisp and delicious, so it is loved by people [2][3]. Morchella has great value in medicines and health foods, and has immunomodulatory [4] and antioxidant activities [5], so the demand is increasing.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Morchella Pathogenic Fungi and Determination of In Vitro Antibacterial Activity of 30 Plant Essential Oils

et al. 2019

E3S Web Conf.

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In order to develop natural antistaling agent for Morchella preservation, reduce environmental pollution problems. In this experiment, the fungus pathogenic fungi were isolated and identified, and the antibacterial activity of the pathogen was determined by using 30 plant essential oils. The results showed that the fungal strain YDJ-S was isolated from the naturally occurring Morchella, belonging to the Fusarium proliferatum, which showed obvious pathogenicity. In vitro antibacterial experiments of essential oils show that in 30 kinds of essential oils, five essential oils of Basil, Cinnamon, Litsea cubeba, Clove and Garlic have obvious inhibitory effect on strain YDJ-S, and the inhibition rate is 100% at 1000 μl/L. Basil essential oil has the most obvious inhibitory effect on the minimum inhibitory concentration and minimum bactericidal concentration of strain YDJ-S, the minimum inhibitory concentration is 250 μl/L, and the minimum bactericidal concentration is 1000 μl/L, to lay the theoretical foundation for further research.

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Metabolic and proteomic analysis of morel fruiting body (Morchella importuna)

Cited by 52 publications

References 32 publications

Analysis of tartary buckwheat ( Fagopyrum tataricum ) seed proteome using offline two‐dimensional liquid chromatography and tandem mass spectrometry

Analysis of tartary buckwheat ( Fagopyrum tataricum ) seed proteome using offline two‐dimensional liquid chromatography and tandem mass spectrometry

In‐depth mapping of the proteome of Tibetan pig tenderloin ( longissimus dorsi ) using offline high‐pH reversed‐phase fractionation and LC‐MS/MS

Isolation and Identification of Morchella Pathogenic Fungi and Determination of In Vitro Antibacterial Activity of 30 Plant Essential Oils

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