In order to develop natural antistaling agent for Morchella preservation, reduce environmental pollution problems. In this experiment, the fungus pathogenic fungi were isolated and identified, and the antibacterial activity of the pathogen was determined by using 30 plant essential oils. The results showed that the fungal strain YDJ-S was isolated from the naturally occurring Morchella, belonging to the Fusarium proliferatum, which showed obvious pathogenicity. In vitro antibacterial experiments of essential oils show that in 30 kinds of essential oils, five essential oils of Basil, Cinnamon, Litsea cubeba, Clove and Garlic have obvious inhibitory effect on strain YDJ-S, and the inhibition rate is 100% at 1000 μl/L. Basil essential oil has the most obvious inhibitory effect on the minimum inhibitory concentration and minimum bactericidal concentration of strain YDJ-S, the minimum inhibitory concentration is 250 μl/L, and the minimum bactericidal concentration is 1000 μl/L, to lay the theoretical foundation for further research.
To determine the antibacterial effect of 34 plant essential oils on Alternaria alternata, 34 plant essential oils such as asarum essential oil, garlic essential oil, and mustard essential oil are used as inhibition agents to isolate A. alternata from citrus as indicator bacteria, through the bacteriostasis test and drug susceptibility test, the types of essential oils with the best inhibitory effect were screened and their concentration was determined. The results showed that the best inhibition effect was mustard essential oil with a minimum inhibitory concentration of 250 μl/L and a minimum bactericidal concentration of 250 μl/L. Followed by the Litsea cubeba essential oil and basil oil, the minimum inhibitory concentration is 500 μl/L.
To extend the storage period and shelf life of Morchella and reduce environmental pollution problems, in this experiment, pathogenic fungi were isolated and identified from the naturally-occurring Morchella, and the antibacterial activity of 22 kinds of plant essential oils was determined by the plate dilution antibacterial method. The results showed that a strong pathogenic fungus strain YDJ-P was isolated and identified from the naturally-occurring Morchella, and it was identified as Rhizopus stolonifer based on morphology and molecular biology. The antibacterial activity of 22 essential oils showed that the essential oils of Clove, Styrax and Spearmint had obvious inhibitory effect on the strain YDJ-P, and the inhibitory rate was 100 % at a concentration of 1000 μl/L. The bacteriostatic rate of Patchouli, Inula, Oregano, Neroli and Myrrh essential oils against strain YDJ-P were significantly different from that of completely bacteriostatic essential oils (P < 0.01), all >50%.
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