Metabolic and mitogenic effects of IGF-II in rainbow trout (Oncorhynchus mykiss) myocytes in culture and the role of IGF-II in the PI3K/Akt and MAPK signalling pathways

Codina, Marta; serrana, Daniel García de la; Sánchez-Gurmaches, Joan; Montserrat, Núria; Chistyakova, O. V.; Navarro, Isabel; Gutiérrez, Joaquím

doi:10.1016/j.ygcen.2008.04.009

Cited by 98 publications

(72 citation statements)

References 61 publications

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“…Mammalian IGF-I and TNF peptides are effective in fish systems in vitro, and numerous examples have been reported (Duan 1998, Albalat et al 2005, Saera-Vila et al 2007, Codina et al 2008. Fish and mammalian IGF-I also give similar results in binding studies (Leibush et al 1996, Navarro et al 1999.…”

Section: Discussionmentioning

confidence: 79%

Regulation of proliferation and differentiation of adipocyte precursor cells in rainbow trout (Oncorhynchus mykiss)

Bouraoui¹,

Gutiérrez²,

Navarro³

2008

Journal of Endocrinology

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Here, we describe optimal conditions for the culture of rainbow trout (Oncorhynchus mykiss) pre-adipocytes obtained from adipose tissue and their differentiation into mature adipocytes, in order to study the endocrine control of adipogenesis. Pre-adipocytes were isolated by collagenase digestion and cultured on laminin or 1% gelatin substrate. The expression of proliferating cell nuclear antigen was used as a marker of cell proliferation on various days of culture. Insulin growth factor-I stimulated cell proliferation especially on days 5 and 7 of culture. Tumor necrosis factor a (TNFa) slightly enhanced cell proliferation only at a low dose. We verified the differentiation of cells grown in specific medium into mature adipocytes by oil red O (ORO) staining. Quantification of ORO showed an increase in triglycerides throughout culture.Immunofluorescence staining of cells at day 11 revealed the expression of CCAAT/enhancer-binding protein and peroxisome proliferator-activator receptor g, suggesting that these transcriptional factors are involved in adipocyte differentiation in trout. We also examined the effect of TNFa on the differentiation of these adipocytes in primary culture. TNFa inhibited the differentiation of these cells, as indicated by a decrease in glycerol-3-phosphate dehydrogenase activity, an established marker of adipocyte differentiation. In conclusion, the culture system described here for trout pre-adipocytes is a powerful tool to study the endocrine regulation of adipogenesis in this species.

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Section: Discussionmentioning

confidence: 79%

Regulation of proliferation and differentiation of adipocyte precursor cells in rainbow trout (Oncorhynchus mykiss)

Bouraoui¹,

Gutiérrez²,

Navarro³

2008

Journal of Endocrinology

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“…While barramundi and salmon Igf1 were more potent in stimulating protein synthesis than Igf2 in rat myoblasts (Degger et al 2001, Wilkinson et al 2004, injection of homologous Igf2 resulted in similar elevations in weight gain in tilapia as injection with Igf1 (Chen et al 2000). Furthermore, Igf2 has recently been shown to have significant mitogenic effects in trout myocytes (Codina et al 2008). In HSB, total hepatic igf2 mRNA levels fell dramatically during feed deprivation and rose rapidly upon realimentation, eventually exceeding those of control fish by day 21 of the experiment.…”

Section: Discussionmentioning

confidence: 93%

Regulation of endocrine and paracrine sources of Igfs and Gh receptor during compensatory growth in hybrid striped bass (Morone chrysops×Morone saxatilis)

Picha

Turano

Tipsmark

et al. 2008

Journal of Endocrinology

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Compensatory growth (CG) is a period of growth acceleration that exceeds normal rates after animals are alleviated of certain growth-stunting conditions. In hybrid striped bass (HSB, Morone chrysops!Morone saxatilis), 3 weeks of complete feed restriction results in a catabolic state that, when relieved, renders a subsequent phase of CG. The catabolic state was characterized by depressed levels of hepatic Type I and II GH receptor (ghr1, ghr2) and igf1 mRNA, along with considerable decreases in plasma Igf1. The state of catabolism also resulted in significant declines in hepatic igf2 mRNA and in circulating 40 kDa Igfbinding protein (Igfbp). Skeletal muscle expression of ghr2 mRNA was significantly increased. Upon realimentation, specific growth rates (SGRs) were significantly higher than sized-matched controls, indicating a period of CG. Hepatic ghr1, ghr2, igf1 and igf2 mRNA levels along with plasma Igf1 and 40 kDa Igfbp increased rapidly during realimentation. Plasma Igf1 and total hepatic igf2 mRNAwere significantly correlated to SGR throughout the study. Skeletal muscle igf1 mRNA also increased tenfold during CG. These data suggest that endocrine and paracrine/autocrine components of the GH-Igf axis, namely igf1, igf2, and ghr1 and ghr2, may be involved in CG responses in HSB, with several of the gene expression variables exceeding normal levels during CG. We also demonstrate that normalization of hepatic mRNA as a function of total liver production, rather than as a fraction of total RNA, may be a more biologically appropriate method of quantifying hepatic gene expression when using real-time PCR.

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“…In contrast to IGF-I, the information about the organ-specific cells which synthesize IGF-II was limited. Codina et al (Codina et al, 2008) found that IGF-II, like IGF-I, could act not only as a growth factor but also as a metabolic hormone, and also IGF-II gene was controlled by GH (Tse et al, 2003;Vong et al, 2003) in all organs. In our study, the transcripts of IGF-II decreased during the post-hatching development, which was consistent with common carp (Cyprinus carpio) (Vong et al, 2003) channel catfish (Ictalurus punctatus) (Peterson et al, 2004) and gilthead sea bream (S. aurata) (Duguay et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

Zeng

Liu

Wang

et al. 2014

Gene

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Metabolic and mitogenic effects of IGF-II in rainbow trout (Oncorhynchus mykiss) myocytes in culture and the role of IGF-II in the PI3K/Akt and MAPK signalling pathways

Cited by 98 publications

References 61 publications

Regulation of proliferation and differentiation of adipocyte precursor cells in rainbow trout (Oncorhynchus mykiss)

Regulation of proliferation and differentiation of adipocyte precursor cells in rainbow trout (Oncorhynchus mykiss)

Regulation of endocrine and paracrine sources of Igfs and Gh receptor during compensatory growth in hybrid striped bass (Morone chrysops×Morone saxatilis)

Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

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