Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

Zeng, Cong; Liu, Xiao-lian; Wang, Weimin; Tong, Jingou; Luo, Wei; Zhang, Jie; Gao, Zexia

doi:10.1016/j.gene.2013.11.027

Cited by 14 publications

(10 citation statements)

References 50 publications

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“…Moreover, M. amblycephala leptinR had nearest genetic distance with C. idella , followed by C. auratus , C. carpio “ jian ” and D. rerio respectively . These results were in accordance with conventional taxonomy of teleosts, similar results were found in GHR s, IGF s and MSTN s genes of M. amblycephala [ 28 ]. Additionally, two forms of leptinR have been identified in previous studies.…”

Section: Discussionsupporting

confidence: 90%

Leptin Genes in Blunt Snout Bream: Cloning, Phylogeny and Expression Correlated to Gonads Development

Zhao

Zeng

et al. 2015

IJMS

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To investigate the leptin related genes expression patterns and their possible function during the gonadal development in fish, the cDNA and genomic sequences of leptin, leptin receptor (leptinR), and leptin receptor overlapping transcript like-1 (leprotl1) were cloned and their expression levels were quantified in the different gonadal development stages of Megalobrama amblycephala. The results showed that the full length cDNA sequences of leptin, leptinR and leprotl1 were 953, 3432 and 1676 bp, coding 168, 1082, and 131 amino acid polypeptides, and the genomic sequences were 1836, 28,528 and 5480 bp, which respectively had 3, 15 and 4 exons, respectively. The phylogenetic analysis revealed that three genes were relatively conserved in fish species. Quantitative real-time PCR results showed that the three genes were ubiquitously expressed in all examined tissues during the different gonadal development stages. The leptin and leptinR took part in the onset of puberty, especially in female M. amblycephala, by increasing the expression levels in brain during the stage I to III of ovary. The expression levels of leptin and leptinR had significant differences between male and female in hypothalamic-pituitary-gonadal (HPG) axis tissues (p < 0.05). The leptinR had the same variation tendency with leptin, but the opposite changes of expression levels were found in leprotl1, which may resist the expression of leptinR for inhibiting the function of leptin in target organ. These findings revealed details about the possible role of these genes in regulating gonadal maturation in fish species.

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Section: Discussionsupporting

confidence: 90%

Leptin Genes in Blunt Snout Bream: Cloning, Phylogeny and Expression Correlated to Gonads Development

Zhao

Zeng

et al. 2015

IJMS

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“…It is reported that a novel phenotype for Laron dwarfism in Bos indicus cattle was associated with underexpression of GHR 1A mRNA, but not other GHR mRNA variants in the liver [14]. The study of Zeng et al [27] showed that GRH 1 and 2 were correlated with each other in adult tissues of blunt snout bream and megalobrama amblycephala. During development, GHR 1 had a similar expression pattern to GHR 2 during the examination.…”

Section: Discussionmentioning

confidence: 97%

Expression and localisation of FSHR, GHR and LHR in different tissues and reproductive organs of female yaks

Chen¹,

Cui²,

Yu³

2018

Folia Morphol

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“…Growth hormone (GH), a single-chain polypeptide hormone secreted by the pituitary gland of animals, plays an indispensable role in the growth and metabolism of the body by regulating the metabolism of carbohydrates, lipids, and proteins [ 16 , 17 ]. As the main hormone of the growth axis, GH plays an important role in regulating the growth and development of animals [ 18 ]. Growth hormone receptor (GHR) is the receptor protein of GH.…”

Section: Introductionmentioning

confidence: 99%

Effects of zearalenone on the localization and expression of the growth hormone receptor gene in the uteri of post-weaning piglets

Zhou

Yang

et al. 2018

Asian-Australas J Anim Sci

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ObjectiveIn this study, we investigated the adverse effects of dietary zearalenone (ZEA) (0.5 to 1.5 mg/kg diet) on the localization and expression of the growth hormone receptor (GHR) in the uteri of post-weaning gilts and explored alternative mechanism of the reproductive toxicity of ZEA on piglets.MethodsA total of forty healthy piglets (Duroc×Landrace×Large White) aged 28 d were selected for study. Piglets were transferred to single cages after 10 days’ adaptation on an obstetric table. The animals were allocated to one of four treatments: a normal basal diet supplemented with 0 (Control), 0.5 (ZEA0.5), 1.0 (ZEA1.0), or 1.5 (ZEA1.5) mg/kg purified ZEA, and fed for 35 d after the 10-d adaptation. Analyzed ZEA concentrations in the diets were 0, 0.52±0.07, 1.04±0.03, and 1.51±0.13 mg/kg, respectively. At the end of the feeding trial, piglets were euthanized after being fasted for 12 h. Two samples of uterine tissue from each pig were rapidly collected, one of which was stored at −80°C for analysis of the relative mRNA and protein expression of GHR, and the second was promptly fixed in Bouin’s solution for immunohistochemical analysis.ResultsThe relative weight of the uteri and thickness of the myometrium and endometrium increased linearly (p<0.001) and quadratically (p<0.001) with an increasing level of ZEA. The results of immunohistochemical analysis indicated that GHR immunoreactive substance was mainly localizated in the cytoplasm of uterine smooth muscle, glandular epithelial, luminal epithelial, stromal, and vascular endothelial cells. In contrast, nuclear staining was rarely observed. The immunoreactive integrated optic density of GHR in the myometrium, luminal epithelium, glandular epithelium, and whole uteri of weaning gilts increased linearly (p<0.001) and quadratically (p<0.05) with an increasing level of ZEA. The mRNA and protein expression of GHR in the uteri of weaning gilts increased linearly (p<0.001) and quadratically (p<0.05) with an increasing level of ZEA.ConclusionIn conclusion, ZEA at a concentration of 0.5 mg/kg was sufficient to significantly thicken the myometrium and endometrium, and at a concentration of 1.0 mg/kg induced a high level of GHR expression to promote growth and development of the uteri. This revealed an alternative molecular mechanism whereby ZEA induces growth and development of the uteri and provides a theoretical basis for the revision of Chinese feed hygiene standards.

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Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

Cited by 14 publications

References 50 publications

Leptin Genes in Blunt Snout Bream: Cloning, Phylogeny and Expression Correlated to Gonads Development

Leptin Genes in Blunt Snout Bream: Cloning, Phylogeny and Expression Correlated to Gonads Development

Expression and localisation of FSHR, GHR and LHR in different tissues and reproductive organs of female yaks

Effects of zearalenone on the localization and expression of the growth hormone receptor gene in the uteri of post-weaning piglets

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