Metabolic and Functional Characterization of Human Adipose-Derived Stem Cells in Tissue Engineering

Mischen, Blaine T.; Follmar, Keith E.; Moyer, Kurtis E.; Buehrer, Benjamin M.; Olbrich, Kevin C.; Levin, L. Scott; Klitzman, Bruce; Erdmann, Detlev

doi:10.1097/prs.0b013e318180ec9f

Cited by 44 publications

(46 citation statements)

References 30 publications

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“…We have previously shown that reductions in the oxygen availability affects chondrogenic and adipogenic differentiation of ASCs and even influences their electrophysiological properties (Refs 2, 104, 105).The values around 1% probably represent an oxygen borderline zone and further dramatic lowering, as has been, for example, shown for 0.1% (Ref. 89), may seriously impair cell proliferation. From the point of final clinical application, it is of interest that the secretion of the proangiogenic factor VEGF appears to increase in inverse proportion to oxygen (Ref.…”

Section: Hypoxiamentioning

confidence: 91%

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Porsborg

Zachar

Boucher

et al. 2015

Expert Rev. Mol. Med.

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Since the discovery of adipose-derived stem cells (ASCs), there have been high expectations of their putative clinical use. Recent advances support these expectations, and it is expected that the transition from pre-clinical and clinical studies to implementation as a standard treatment modality is imminent. However ASCs must be isolated and expanded according to good manufacturing practice guidelines and a basic assurance of quality, safety, and medical effectiveness is needed for authorisation by regulatory agencies, such as European Medicines Agency and US Food and Drug Administration. In this review, a collection of studies investigating the influence of different steps of the isolation and expansion protocol on the yield and functionality of ASCs has been presented in an attempt to come up with best recommendations that ensure potential beneficial clinical outcome of using ASCs in any therapeutic setting. If the findings confirm the initial observations of beneficial effects of ASCs, the path is paved for implementing these ASC-based therapies as standard treatment options.

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Section: Hypoxiamentioning

confidence: 91%

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Porsborg

Zachar

Boucher

et al. 2015

Expert Rev. Mol. Med.

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“…Remarkable progress has been made with respect to the culture of hASCs on both natural and synthetic scaffolds, in vitro and in vivo, and for various culture time frames. In addition to exploring hASC viability and differentiation capacity on various materials, 18,38,50,59,77,[130][131][132][133] contributions of hASCs toward vascularization 36,70,85,134 and wound healing 135 have been investigated.…”

Section: Cell Sources For Adipose Tissue Engineeringmentioning

confidence: 99%

“…Human bone-marrow-derived mesenchymal stem cells 37,39,42,43,49,66,76,90,122 Human adipose-derived mesenchymal stem cells 7,18,36,38,41,44,50,54,59,77,83,85,87,91,103,126,[130][131][132][133][134][135] Human pluripotent cells 145 studies have been conducted to investigate the differentiation potential of embryonic cells. [139][140][141][142][143] In particular, the derivation of multipotent MSCs from human ESCs (hESCs) has been demonstrated, and these cells were able to differentiate into adipocytes.…”

Section: Cell Source Referencesmentioning

confidence: 99%

Adipose Tissue Engineering for Soft Tissue Regeneration

Choi

Gimble

Lee

et al. 2010

Tissue Engineering Part B: Reviews

213

183

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Current treatment modalities for soft tissue defects caused by various pathologies and trauma include autologous grafting and commercially available fillers. However, these treatment methods present a number of challenges and limitations, such as donor-site morbidity and volume loss over time. As such, improved therapeutic modalities need to be developed. Tissue engineering techniques offer novel solutions to these problems through development of bioactive tissue constructs that can regenerate adipose tissue in both structure and function. Recently, a number of studies have been designed to explore various methods to engineer human adipose tissue. This review will focus on these developments in the area of adipose tissue engineering for soft tissue replacement. The physiology of adipose tissue and current surgical therapies used to replace lost tissue volume, specifically in breast tissue, are introduced, and current biomaterials, cell sources, and tissue culture strategies are discussed. We discuss future areas of study in adipose tissue engineering.

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“…21,22 Then it was used for cosmetic surgery, initially facial and gluteal contours. 6,[21][22][23][24] Currently, its use for breast surgery, both reconstructive and aesthetic, is in vogue. 25 At the beginning, several critics of this technique focused on the high percentage of fat absorption, but later, several authors improved the fat preparation technique, the injection procedure, and the plane used to place the fat graft.…”

Section: Since Description On Autologous Fat Transfer By Van Dermentioning

confidence: 99%

“…[5][6][7] Due to regenerative stem cell effect of fat, author consider AFT as scar remodelling but with that feasibility, efficacy and acceptance of fat transfer is of same importance.…”

Section: Introductionmentioning

confidence: 99%

An intervention study of autologous fat transfer for scar tissue rejuvenation in the setup

Parikh

Darshan

2018

Int Surg J

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Background: There are lot of work going on AFT procedures. This procedure has been taken recent advances nowadays and very helpful in many conditions to rejuvenate and restoration of damaged tissues. The use of autologous fat for soft tissue augmentation seems attractive because of its relatively ubiquitous availability. Autologous fat transfer is a technique shown to be beneficial as a reconstructive procedure for patients with volume loss of tissue due to disease, trauma, and congenital defects.Methods: In the present study, 20 patients were taken as sample size. It is an intervention study. Whole procedure has been taken as day case which included local anaesthesia and patient received all the standard monitoring, medications, safety precautions and care that is provided for any patient undergoing any surgery, including antibiotics, pain medicine and other supportive care as needed. The Autologous fat was harvested by surgeon via syringe aspiration technique with a long atraumatic cannula. After processing, the material was injected immediately under designated skin grafts/scars using a small needle. Scar was cleansed with normal saline and dry dressing is applied for a day. Then patient was discharged on the same day.Results: Results obtained were efficacious with 60 to 80% improvement in all parameters in 15-20 days after the procedure.Conclusions: AFT procedure was found feasible, efficacious and accepted in patients with scar. It brings scar tissue rejuvenation up to great extent.

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Metabolic and Functional Characterization of Human Adipose-Derived Stem Cells in Tissue Engineering

Cited by 44 publications

References 30 publications

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Adipose Tissue Engineering for Soft Tissue Regeneration

An intervention study of autologous fat transfer for scar tissue rejuvenation in the setup

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