Meta-analysis reveals key features of the improved drought tolerance of plants overexpressing NAC transcription factors

Figueroa, Nicolás; Lodeyro, Anabella F.; Carrillo, Néstor; Gómez, Rodrigo

doi:10.1016/j.envexpbot.2021.104449

Cited by 13 publications

(14 citation statements)

References 87 publications

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“…There may be differences in anthocyanin biosynthesis between heterologous expression and homologous expression. This result is interesting, but similar observations have been reported for WRKY and CBF/DREB TFs ( Dong et al, 2017 ; Guo et al, 2019 ; Figueroa et al, 2021 ). These results also signify the importance of the expression systems of plants in experimental designs and results.…”

Section: Discussionsupporting

confidence: 88%

“…The identity of the donor and receptor plants influenced the efficacy of MYB transformation on each effect size. Arabidopsis is the most studied model system ( Ma et al, 2017 ; Figueroa et al, 2021 ), providing independent and original research on anthocyanidin biosynthesis ( Fan et al, 2020 ; Zhou et al, 2020 ). However, the meta-analysis demonstrates that 92 studies came from Arabidopsis , which was the largest donor genus but not the best.…”

Section: Discussionmentioning

confidence: 99%

“…For most plants, regeneration and transformation remain arduously challenging even after more than 30 years of continuous work, and only a handful of species can be transformed ( Altpeter et al, 2016 ; Maher et al, 2020 ). Nicotiana and Arabidopsis are easy to induce transformants, and to evaluate the function of MYB genes in anthocyanin biosynthesis ( Shen et al, 2019 ; Zhou et al, 2020 ; Figueroa et al, 2021 ). The meta-analysis indicated that more than half of the studies ectopically overexpressed MYB , which significantly increased the contents of anthocyanin, delphinidin, proanthocyanidin and kaempferol and promoted the expression of PAL , F3′5′H , ANS , and LAR .…”

Section: Discussionmentioning

confidence: 99%

“…Meta-analysis can be used for the statistical analysis of a massive number of datasets obtained from individual researches to determine the effect of the various variables on a number of phenotypic responses ( Borenstein et al, 2009 ; Zhang et al, 2020 ). Meta-analysis is a powerful and conducive approach to classify and identify biological responses to regulatory genes, including cation/proton antiporter 1 ( CPA1 ) genes, C-repeat/dehydration responsive element binding proteins ( CBF/DREB ), and the NAC ( NAM/ATAF/CUC ) family ( Dong et al, 2017 , 2018 ; Ma et al, 2017 ; Figueroa et al, 2021 ). The previous meta-analysis mainly focused on the effect of overexpression of family genes on abiotic stress in plants, but no report has revealed the effect of MYB genes overexpression on anthocyanin biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

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Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis

et al. 2021

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MYBs (v-myb avian myeloblastosis viral oncogene homologs) are important transcriptional regulators that play critical roles in the regulation of anthocyanin biosynthesis. The overexpression of MYB genes has been reported in different plant species. However, the inconsistent strategies to assess transgenic plants have made it difficult to explain the complex mechanisms of regulation of anthocyanin biosynthesis by MYBs. We report here a meta-analysis of 608 studies from 206 publications assessing the effects of MYB overexpression on anthocyanins and evaluate the experimental variables that have an influence on transgenic plant performance. We found that MYB expression enhanced the magnitude of 20 out of 26 examined plant parameters by at least of 21% and reduced the magnitude of 1 indicator by at least 37%. We explored the variety of moderating variables causing these variations. A deeper color induced by MYBs caused higher plant attributes as compared to normal color changes. MYB genes from dicots stimulated the accumulation of anthocyanins, flavonols and impacted the expressions of PAL, CHS, CHI, FLS, F3′5′H, ANS, UFGT, and ANR as compared to monocots. Heterologous expression and homologous expression showed a great difference in anthocyanin biosynthesis. Transient gene transformation had a significant effect on the expression of flavonoid biosynthetic genes, and stable transformation had a significant effect on flavonoid accumulation. Stress could result in a significantly increased accumulation of flavonoids, especially anthocyanin, flavonol, and proanthocyanidin. Our study, thus, provides new insights into the function of MYBs in the regulatory mechanisms of flavonoid biosynthesis and the use of genetic engineering for improving anthocyanins contents.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis

et al. 2021

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“…Finally, the SNAC3 gene in rice targets a ROS-scavenging gene, and SNAC3 overexpression upregulates this target gene ( Fang et al, 2015 ). Previous studies suggest that, when plants are under stress, the NAC family act as transcriptional activators or repressors of their downstream genes, such as NAC regulatory network interactions with jasmonic acid-, salicylic acid- and ethylene-mediated stress responses via both ABA-dependent and independent pathways by binding to the promoter of Early Response to Dehydration 1 (ERD1; Figueroa et al, 2021 ). These regulatory networks can reduce transpiration rate by promoting plant root elongation, reducing H 2 O 2 and MDA accumulation, and increasing leaf water content.…”

Section: Discussionmentioning

confidence: 99%

NAC Transcription Factor TwNAC01 Positively Regulates Drought Stress Responses in Arabidopsis and Triticale

et al. 2022

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The NAC transcription factors play important roles in regulating plant growth, development, and senescence, and responding to biotic and abiotic stressors in plants. A novel coding sequence (1,059 bp) was cloned from hexaploid triticale in this study. The putative protein (352 amino acids) encoded by this sequence was over 95% similar to the amino acid sequence of a NAC protein from Aegilops tauschii (XP020161331), and it formed a clade with Ae. tauschii, durum wheat, and barley. The putative protein contained a conserved nature actomyosin (NAM) domain (129 consecutive amino acids) between the 20th and 148th amino acids at the N-terminus and three transcription activation regions at the C-terminus. The novel gene was identified as a triticale NAC gene localized in the nucleus and designated as TwNAC01 (GenBank accession MG736919). The expression levels of TwNAC01 were the highest in roots, followed by leaves and stems when triticale lines were exposed to drought, polyethylene glycol 6,000 (PEG6000), NaCl, cold, methyl jasmonate (MeJA), and abscisic acid (ABA). Transgenic Arabidopsis thaliana overexpressing TwNAC01 had significantly lower leaf water loss rates and longer roots than wild-type (WT) A. thaliana. Virus-induced silencing of the TwNAC01 gene in triticale delayed root development and decreased length of primary root. Under drought stress, leaves of TwNAC01-silenced triticale had higher levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2), but lower relative water content (RWC), net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, and transpiration rate than the leaves of the WT. Gene overexpression and silencing experiments suggested that TwNAC01 improves plant stress tolerance by increasing root length, regulating the water content of plant leaves by reducing MDA and H2O2 content, and adjusting respiration rate. The results suggest that TwNAC01 is a novel NAC transcription factor gene that can be exploited for triticale and cereal improvement.

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Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance

Mano,

Shaikh,

Widhalm

et al. 2024

Plant Direct

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The transcription factor GT2‐LIKE 1 (GTL1) has been implicated in orchestrating a transcriptional network of diverse physiological, biochemical, and developmental processes. In response to water‐limiting conditions, GTL1 is a negative regulator of stomatal development, but its potential rolein other water‐deficit responses is unknown. We hypothesized that GTL1 regulates transcriptome changes associated with drought tolerance over leaf developmental stages. To test the hypothesis, gene expression was profiled by RNA‐seq analysis in emerging and expanding leaves of wild‐type and a drought‐tolerant gtl1‐4 knockout mutant under well‐watered and water‐deficit conditions. Our comparative analysis of genotype‐treatment combinations within leaf developmental age identified 459 and 1073 differentially expressed genes in emerging and expanding leaves, respectively, as water‐deficit responsive GTL1‐regulated genes. Transcriptional profiling identified a potential role of GTL1 in two important pathways previously linked to drought tolerance: flavonoid and polyamine biosynthesis. In expanding leaves, negative regulation of GTL1 under water‐deficit conditions promotes biosynthesis of flavonoids and anthocyanins that may contribute to drought tolerance. Quantification of polyamines did not support a role for GTL1 in these drought‐responsive pathways, but this is likely due to the complex nature of polyamine synthesis and turnover. Our global transcriptome analysis suggests that transcriptional repression of GTL1 by water deficit allows plants to activate diverse pathways that collectively contribute to drought tolerance.

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Meta-analysis reveals key features of the improved drought tolerance of plants overexpressing NAC transcription factors

Cited by 13 publications

References 87 publications

Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis

Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis

NAC Transcription Factor TwNAC01 Positively Regulates Drought Stress Responses in Arabidopsis and Triticale

Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance

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