Meta-analysis and co-expression analysis revealed stable QTL and candidate genes conferring resistances to Fusarium and Gibberella ear rots while reducing mycotoxin contamination in maize

Akohoue, Félicien; Miedaner, Thomas

doi:10.3389/fpls.2022.1050891

Cited by 7 publications

(9 citation statements)

References 145 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Environment-specific GER resistance breeding targets should also be defined for a better and more efficient exploitation of the large existing genetic diversity within landraces. In addition, with recent advances and availability of molecular genomic information on the trait [ 27 , 30 , 53 , 54 ], genomics-assisted selection could be applied for accelerated evaluation for GER resistance in maize.…”

Section: Discussionmentioning

confidence: 99%

“…The introgression of GER resistance genes into elite materials could be accelerated through the application of genomics-assisted selection within populations. Considering the complexity and quantitative nature of GER resistance, backcross selection could be combined with genomic selection and prediction which seem to be the most relevant molecular approaches that could be implemented within each population to rapidly improve GER resistance of elite materials as indicated by Akohoue and Miedaner [ 30 ]. Given the existence of significant genotype by environment interaction within the DH populations, the integration of genomics-assisted breeding for increased selection efficiency could be implemented within environment to better exploit QTL by environment interaction and define area-specific breeding targets.…”

Section: Discussionmentioning

confidence: 99%

“…Gaikpa et al [ 27 ] reported eight QTL significantly associated with GER resistance in “Kemater Landmais Gelb” (KE, from Austria) landrace. Recently, Akohoue and Miedaner [ 30 ] conducted a quantitative trait loci (QTL) meta-analysis which revealed that diverse sources of resistance, including the KE landrace, contributed to the identification of 40 meta-QTL harbouring several putative resistance genes. This demonstrates that the European flint landraces represent important sources of resistance which could be explored for their introgression into elite cultivars.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effectiveness of introgression of resistance loci for Gibberella ear rot from two European flint landraces into adapted elite maize (Zea mays L.)

Akohoue,

Koch,

Lieberherr

et al. 2023

PLoS ONE

Self Cite

View full text Add to dashboard Cite

European flint landraces are a major class of maize possessing favorable alleles for improving host resistance to Gibberella ear rot (GER) disease which reduces yield and contaminates the grains with mycotoxins. However, the incorporation of these landraces into breeding programs requires a clear understanding of the effectiveness of their introgression into elite materials. We evaluated 15 pre-selected doubled haploid (DH) lines from two European flint landraces, “Kemater Landmais Gelb” (KE) and “Petkuser Ferdinand Rot” (PE), together with two adapted elite flint lines and seven standard lines for GER severity as the main trait, and several adaptation traits (plant height, days to silking, seed-set, plant vigor) across four environments. From this evaluation, three KE DH lines and one PE DH line, with the lowest GER severity, were selected and used as donor parents that were crossed with the two adapted and GER susceptible flint lines (Flint1 and Flint2) to develop six bi-parental DH populations with 34−145 DH lines each. Each DH population was evaluated across two locations. Correlations between GER severity, which was the target trait, and adaptation traits were weak (−0.02 to 0.19). GER severity of lines from PE landrace was on average 2-fold higher than lines from KE landrace, indicating a clear superiority of the KE landrace lines. Mean GER severity of the DH populations was 39.4−61.0% lower than the adapted elite flint lines. All KE-derived DH populations were on average more resistant (27.0−36.7%) than the PE-derived population (51.0%). Highly resistant lines (1.3−5.2%) were found in all of the populations, suggesting that the DH populations can be successfully integrated into elite breeding programs. The findings demonstrate that selected KE landrace lines used as donors were effective in improving GER resistance of the adapted elite inbreds.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effectiveness of introgression of resistance loci for Gibberella ear rot from two European flint landraces into adapted elite maize (Zea mays L.)

Akohoue,

Koch,

Lieberherr

et al. 2023

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…No preference of individual maturity groups is given what is similar to the maize mycotoxin data (Figure S1). The inheritance of Fusarium ear rot in maize is also caused by many low‐effect QTLs that are population and environment specific for the most part (Akohoue & Miedaner, 2022; Gaikpa & Miedaner, 2019).…”

Section: Detection Of Fhb Resistance In Other Cereal Cropsmentioning

confidence: 99%

The importance of Fusarium head blight resistance in the cereal breeding industry: Case studies from Germany and Austria

2023

Self Cite

View full text Add to dashboard Cite

Fusarium head blight (FHB) resistance in wheat and triticale has a high priority in the European Union because of the strict guidelines for the major mycotoxins deoxynivalenol (DON) and zearalenone (ZON) and the admission policy of the regulatory authorities. Potentially 70% of the arable land in Germany and about 60% in Austria can be affected by Fusarium. Although epidemics occur only in some years and/or some regions, DON and ZON are detected every year in varying amounts in wheat, rye and maize. Despite a high significance of FHB resistance in breeding companies, as validated by a recent survey, breeding progress in wheat is basically absent for FHB resistance in both countries. The main reasons are the complex inheritance of FHB resistance and the high proportions of the dwarfing allele Rht‐D1b in high‐yielding varieties promoting susceptibility. Despite this, some varieties with high FHB resistance (score 2–3 on the 1–9 scale) have been released that account, however, only for 11% and 18% of the multiplication area in Germany and Austria, respectively. For triticale, an official testing system for FHB resistance in terms of DON content exists in Germany and Austria, but not for the other cereals. Susceptibility to maize ear rot has been described in Austria, but not in Germany. Additionally, a testing system for stalk rot resistance in both countries should be established.

show abstract

“…Similar to placental mammals ( Chettoor et al., 2016 ), in flowering plants, an embryo develops inside the surrounding tissue of the mother, and its well-being depends on the protection and nurture provided by the maternal tissue, including against pathogens ( Robert et al., 2018 ; Nguyen et al., 2021 ). Quantitative trait loci (QTLs) have been identified that improve style resistance to disease ( Reid et al., 1996a ; Reid et al, 1996b ; Akohoue and Miedaner, 2022 ), suggestive that the maternal parent may protect the stigma-style passage, but little appears to be known about the underlying mechanism(s).…”

Section: Introductionmentioning

confidence: 99%

Bacteria existing in pre-pollinated styles (silks) can defend the exposed male gamete fertilization channel of maize against an environmental Fusarium pathogen

Shrestha,

Limay-Rios,

Brettingham

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

In flowering plants, fertilization requires exposing maternal style channels to the external environment to capture pollen and transmit its resident sperm nuclei to eggs. This results in progeny seed. However, environmental fungal pathogens invade developing seeds through the style. We hypothesized that prior to environmental exposure, style tissue already possesses bacteria that can protect styles and seed from such pathogens. We further hypothesized that farmers have been inadvertently selecting immature styles over many generations to have such bacteria. We tested these hypotheses in maize, a wind-pollinated crop, which has unusually long styles (silks) that are invaded by the economically-important fungal pathogen Fusarium graminearum (Fg). Here, unpollinated silk-associated bacteria were cultured from a wild teosinte ancestor of maize and diverse maize landraces selected by indigenous farmers across the Americas, grown in a common Canadian field for one season. The bacteria were taxonomically classified using 16S rRNA sequencing. In total, 201 bacteria were cultured, spanning 29 genera, 63 species, and 62 unique OTUs, dominated by Pseudomonas, Pantoea and Microbacterium. These bacteria were tested for their ability to suppress Fg in vitro which identified 10 strains belonging to 6 species: Rouxiella badensis, Pantoea ananatis, Pantoea dispersa, Pseudomonas koreensis, Rahnella aquatilis, and Ewingella americana. Two anti-Fg strains were sprayed onto silks before/after Fg inoculation, resulting in ≤90% reductions in disease (Gibberella ear rot) and 70-100% reductions in associated mycotoxins (deoxynivalenol and zearalenone) in progeny seeds. These strains also protected progeny seeds post-harvest. Confocal fluorescent imaging showed that one silk bacterium (Rouxiella AS112) colonized susceptible entry points of Fg on living silks including stigmatic trichomes, wounds, and epidermal surfaces where they formed thick biofilms. Post-infection, AS112 was associated with masses of dead Fg hyphae. These results suggest that the maize style (silk) is endowed with potent bacteria from the mother plant to protect itself and progeny from Fusarium. The evidence suggests this trait may have been selected by specific indigenous peoples, though this interpretation requires further study.

show abstract

Meta-analysis and co-expression analysis revealed stable QTL and candidate genes conferring resistances to Fusarium and Gibberella ear rots while reducing mycotoxin contamination in maize

Cited by 7 publications

References 145 publications

Effectiveness of introgression of resistance loci for Gibberella ear rot from two European flint landraces into adapted elite maize (Zea mays L.)

Effectiveness of introgression of resistance loci for Gibberella ear rot from two European flint landraces into adapted elite maize (Zea mays L.)

The importance of Fusarium head blight resistance in the cereal breeding industry: Case studies from Germany and Austria

Bacteria existing in pre-pollinated styles (silks) can defend the exposed male gamete fertilization channel of maize against an environmental Fusarium pathogen

Contact Info

Product

Resources

About