Met-Anandamidin Koç Spermasının Dondurulmasında Hiperaktivasyon, Kryo-Kapasitasyon ve Akrozom Reaksiyonunun Önlenmesi Üzerine Etkisi

Talebiyan, Reza; Amidi, Fardin; Samini, Morteza; Mirshokraei, Pezhman; Dehkordi, Saied Habibian

doi:10.9775/kvfd.2014.12897

Kafkas Univ Vet Fak Derg

2015

DOI: 10.9775/kvfd.2014.12897

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Met-Anandamidin Koç Spermasının Dondurulmasında Hiperaktivasyon, Kryo-Kapasitasyon ve Akrozom Reaksiyonunun Önlenmesi Üzerine Etkisi

Reza Talebiyan¹,

Fardin Amidi²,

Morteza Samini³

et al.

Abstract: Cryopreserved spermatozoon has some defects on membrane causing hyper activation, cryo-capacitation and timeless (early) acrosome reaction that lead to low fertility. While low fertility is generally accepted as a consequence of cryopreservation of sperm cells, a lot of studies are being carried out on cryopreservation techniques to improve spermatozoon membrane integrity and also viability. Some studies point out that using materials such as Cannabinoids can be effective in achieving this aim. The main effect… Show more

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“…It has been alleged that the combination of oleic/linoleic acids with vitamin E and SPPs (6), some other agents, e.g. fatty acid amide hydrolase (FAAH) [32], and ω-3 FА provided protection in frozen-thawed ram sperm.…”

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ADDITIVES USED IN EXTENDERS TO IMPROVE THE FREEZABILITY OF RAM SEMEN IN RECENT YEARS: a mini review

Öztürkler¹,

Ari²

2017

S-h. biol.

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A b s t r a c tOne of the most important reasons why artificial insemination does not spread as much in cattle as sheep is that ram sperm is highly fragile against cryodamage and consequently the optimum fertility results can not be obtained from cervical inseminations compared to laparoscopic insemination. To establish an ideal ram sperm freezing model, many studies have been carried out for more than 60 years and various methods have been tested by making great efforts. One of the topics where these tests have been done intensively in recent years are additives used in freezing extenders in order to increase the tolerance of sperm against oxidative stress in frozen-thawed ram semen. In this review, covering the studies carried out between the years 2000-2016, we have mostly compiled the additives used in ram semen freezing media which have given better results compared to others supplements. It is understood that, usually, seminal plasma and proteins, thiol compounds, enzymatic antioxidants, sugars, fatty acids and vitamins have been studied as additive agents in recent years. As a result, this review suggests us that further studies are needed to explore the novel techniques and new additives, and their combinations should be tested in different doses in order to install an ideal cryopreservation template for ram semen. Furthermore, even though addition of various compounds to freezing media have improved freezability of ram semen in experimental conditions, anyway these results must be confirmed in field studies. Therefore, the value of sperm which potential fertility is predicted from laboratory survey must be compared with conception or lambing rates.Keywords: ram semen, additives, freezability, extender While the basic techniques related to sperm freezing have done positive effects and important advances mostly on dairy cattle husbandry, any remarkable success has not been provided in sheep. Substantially, these matter is caused mainly by from two reasons following as: i] intolerance of ram sperm against cryopreservation, ii] the physiological and anatomic factors in sperm transport. It is well known subject for a long time that freezing and thawing of ram sperm have caused significant membran and genetic damage [1]. As cervical insemination has given poor fertility results compared to laparascopic inseminations, artificial insemination with frozen semen has not become widespread in sheep husbandry [2]. On the other hand, the expenses of ram semen freezing are almost 20 times more than those of bull semen freezing cost [3]. В Recently, investigators have focused mostly on especially composition, determination of in vivo properties and effects of different cryoprotectants, antioxidants and specific supplements in sperm freezing [4].We understood from literature documented here, no comprehensive review of the researches on frozen ram sperm has been found from that date into the past 16 years. During 2000s, the attentions of researchers have mostly concentrated on additives for improving membrane repair and ...

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confidence: 99%

ADDITIVES USED IN EXTENDERS TO IMPROVE THE FREEZABILITY OF RAM SEMEN IN RECENT YEARS: a mini review

Öztürkler¹,

Ari²

2017

S-h. biol.

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Met-Anandamidin Koç Spermasının Dondurulmasında Hiperaktivasyon, Kryo-Kapasitasyon ve Akrozom Reaksiyonunun Önlenmesi Üzerine Etkisi

Cited by 1 publication

References 37 publications

ADDITIVES USED IN EXTENDERS TO IMPROVE THE FREEZABILITY OF RAM SEMEN IN RECENT YEARS: a mini review

ADDITIVES USED IN EXTENDERS TO IMPROVE THE FREEZABILITY OF RAM SEMEN IN RECENT YEARS: a mini review

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