Messenger RNA expression of periostin and Twist transiently decrease by occlusal hypofunction in mouse periodontal ligament

Afanador, Elaine; Yokozeki, Masahiko; Oba, Yasuo; Kitase, Yukiko; Takahashi, Takumi; Kudo, Akira; Moriyama, Keiji

doi:10.1016/j.archoralbio.2005.04.002

Cited by 52 publications

(43 citation statements)

References 34 publications

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“…We demonstrated that Twist was detected sparsely within mouse periodontium and relatively intense staining was specifically distributed along the alveolar bone surface in PDL immunohistochemically (Fig. 1A), which is consistent with previous report showing that Twist and its target gene periostin are expressed in mice PDL [Afanador et al, 2005]. It is also known that a calvarial suture is formed where two ossification centers expand and confront each other under the regulation of growth factors [Kim et al, 1998;Rice et al, 2005].…”

Section: Discussionsupporting

confidence: 93%

“…Rajshankar et al [1998] reported that BMP-7 promoted the proliferation but did not significantly induce osteogenic differentiation of cells in mouse periodontal wound model. Occlusal hypofunction decreased Twist gene expression [Afanador et al, 2005] and increased BMP-2-induced transient ankylosis [King and Hughes, 1999] in rodent PDL, suggesting Twist plays a role in maintenance of PDL. It is, therefore, important to understand the mechanisms of PDL to resist mineralization.…”

Section: Discussionmentioning

confidence: 98%

“…Bialek et al [2004] reported that Twist interacted with the DNA-binding domain of Runx2 to inhibit its function, without altering its expression. Afanador et al [2005] reported that Twist was expressed in mice PDL and its expression was transiently decreased by occlusal hypofunction, suggesting the functional role of Twist in PDL. However, the function of Twist in human PDL is entirely unknown.…”

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confidence: 97%

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Twist negatively regulates osteoblastic differentiation in human periodontal ligament cells

Komaki

Karakida

Abé

et al. 2006

J of Cellular Biochemistry

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Periodontal ligament (PDL) is a thin fibrous connective tissue located between two mineralized tissues, alveolar bone and cementum, which maintains a constant width physiologically. The mechanisms by which PDL resists mineralization are not well understood. Twist is a basic helix loop helix protein that plays a central role in regulation of early osteogenesis. We investigated the localization of Twist in PDL and compared the expression of Twist and osteoblast-related genes in PDL cells with those in osteoblast-like cells in the presence or absence of recombinant human bone morphogenetic protein (BMP)-2. Histochemical analysis showed that Twist was expressed along alveolar bone surface in PDL. PDL cells constitutively expressed Twist gene and the expression level was higher than that in osteoblast-like cells. In osteoblast-like cell culture, BMP-2 enhanced osteoblast-related gene expression, while Twist expression was slightly decreased. In contrast, BMP-2 increased runt-related transcription factor (Runx)-2, but failed to enhance alkaline phosphatase (ALP) and osteocalcin (OCN) gene expression in PDL cells. Interestingly, unlike in osteoblast-like cells, Twist expression was upregulated by BMP-2 in PDL cells. We transiently knocked down Twist gene in PDL cells using a short interference RNA expression vector (siTwist) and found that ALP, osteopontin (OPN), bone sialoprotein (BSP) genes expression and basal level of ALP activity were slightly increased, whereas Runx2 and OCN genes were not affected. Collectively, these results suggest that Twist may act as a negative regulator of osteoblastic differentiation in PDL cells.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 97%

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Twist negatively regulates osteoblastic differentiation in human periodontal ligament cells

Komaki

Karakida

Abé

et al. 2006

J of Cellular Biochemistry

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“…In situ hybridization and immunohistochemistry showed that periostin is expressed in the PDL, in dental pulp at the sites of hard/soft tissue interfaces in mouse and human tooth [32,33]. Periostin is secreted by fibroblasts and osteoblasts in PDL.…”

Section: Periostin Is Expressed In Bone and Teethmentioning

confidence: 99%

The multiple facets of periostin in bone metabolism

2012

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Periostin is a matricellular glutamate-containing protein expressed during ontogenesis and in adult connective tissues submitted to mechanical strains including bone and, more specifically, the periosteum, periodontal ligaments, tendons, heart valves, or skin. It is also expressed in neoplastic tissues, cardiovascular and fibrotic diseases, and during wound repair. Its biological functions are extensively investigated in fields such as cardiovascular physiology or oncology. Despite its initial identification in bone, investigations of periostin functions in bone-related physiopathology are less abundant. Recently, several studies have analyzed the potential role of periostin in bone biology and suggest that periostin may be an important regulator of bone formation. The aim of this article is to provide an extensive review on the implications of periostin in bone biology and its potential use in benign and metabolic bone diseases.

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“…Moreover, in vitro application of tensional forces to periodontal cells increases Postn expression. In contrast, following in vivo masticatory unloading, Postn mRNA levels decrease (11,12). Postn-deficient mice (Postn Ϫ/Ϫ ) show severe alterations in tooth (incisor) eruption, resulting from a failure to digest collagen fibers in the shear zone of the periodontal ligament (13,14).…”

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confidence: 99%

The Matricellular Protein Periostin Is Required for Sost Inhibition and the Anabolic Response to Mechanical Loading and Physical Activity

Bonnet

Standley

Bianchi

et al. 2009

Journal of Biological Chemistry

184

198

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Messenger RNA expression of periostin and Twist transiently decrease by occlusal hypofunction in mouse periodontal ligament

Cited by 52 publications

References 34 publications

Twist negatively regulates osteoblastic differentiation in human periodontal ligament cells

Twist negatively regulates osteoblastic differentiation in human periodontal ligament cells

The multiple facets of periostin in bone metabolism

The Matricellular Protein Periostin Is Required for Sost Inhibition and the Anabolic Response to Mechanical Loading and Physical Activity

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