Messenger ribonucleic acid content of Bacillus amyloliquefaciens throughout its growth cycle compared with Bacillus subtilis 168

Brown, S.; Coleman, G.

doi:10.1016/0022-2836(75)90353-8

Cited by 17 publications

(12 citation statements)

References 12 publications

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“…As described above, the total mRNA content of B. amyloliquefaciens assayed by hybridization with DNA at a ratio of DNA to RNA of 10:1 doubles as the culture enters stationary phase (31). This increased mRNA pool is composed of equal amounts of exoprotein and cellular protein mRNA, reflecting a 25-fold increase in exo-VOL.…”

Section: Downloaded Frommentioning

confidence: 87%

“…This newly-synthesized mRNA was resolved into exoprotein or cellular protein fractions by measuring the rate of incorporation of labeled valine into extracellular and cellular proteins (assuming that both mRNA species are translated with equal efficiency). The results indicated that the increase in total mRNA observed in late exponentialphase cultures is due almost entirely to a 25fold increase in exoprotein mRNA synthesis (31). This pattern of events has been compared with the synthesis of mRNA in B. subtilis in which a postexponential-phase increase in total mRNA was not observed, presumably because the technique is insufficiently sensitive to detect the small variations in mRNA that would be associated with the low level of exoenzyme synthesis in this organism.…”

Section: Regulation Of Synthesismentioning

confidence: 91%

“…41,1977 on July 6, 2020 by guest http://mmbr.asm.org/ Downloaded from (rRNA) hybridization occurs. Brown and Coleman (31) have used this procedure to assay the mRNA content of B. amyloliquefaciens throughout the growth cycle. During exponential growth, mRNA accounted for a constant proportion (about 3%) of the total RNA in the cell.…”

Section: Regulation Of Synthesismentioning

confidence: 99%

“…Coleman observed a 17% reduction in the total RNA content ofB. subtilis 168 during the transition from exponentialto stationary-phase growth (31). However, although net synthesis stops at to, measurement of the incorporation of radioactive precursors into RNA indicates that it is undergoing a rapid intracellular turnover.…”

Section: Downloaded Frommentioning

confidence: 99%

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Extracellular enzyme synthesis in the genus Bacillus.

Priest¹

1977

Bacteriological Reviews

482

164

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Section: Downloaded Frommentioning

confidence: 87%

Section: Regulation Of Synthesismentioning

confidence: 91%

Section: Regulation Of Synthesismentioning

confidence: 99%

Section: Downloaded Frommentioning

confidence: 99%

See 2 more Smart Citations

Extracellular enzyme synthesis in the genus Bacillus.

Priest¹

1977

Bacteriological Reviews

482

164

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“…Archaea are microscopic, single-celled organisms with no nucleus, no mitochondria and no chloroplasts. Regarding mRNA, they are more similar to bacteria than to eukaryotes: mRNA does not have introns, it is polycistronic, is not modified and does not have long stabilizing poly(A) tails at the 3 0 end (Brown & Coleman, 1975;Brown & Reeve, 1986). However, in Sulfolobus and Methanothermobacter, the existence of an archaeal exosome with characteristics of the eukaryotic exosome was demonstrated (Evguenieva-Hackenberg et al, 2003;Farhoud et al, 2005).…”

Section: Rna-degrading Machinesmentioning

confidence: 99%

The critical role of RNA processing and degradation in the control of gene expression

et al. 2010

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The continuous degradation and synthesis of prokaryotic mRNAs not only give rise to the metabolic changes that are required as cells grow and divide but also rapid adaptation to new environmental conditions. In bacteria, RNAs can be degraded by mechanisms that act independently, but in parallel, and that target different sites with different efficiencies. The accessibility of sites for degradation depends on several factors, including RNA higher-order structure, protection by translating ribosomes and polyadenylation status. Furthermore, RNA degradation mechanisms have shown to be determinant for the post-transcriptional control of gene expression. RNases mediate the processing, decay and quality control of RNA. RNases can be divided into endonucleases that cleave the RNA internally or exonucleases that cleave the RNA from one of the extremities. Just in Escherichia coli there are >20 different RNases. RNase E is a single-strand-specific endonuclease critical for mRNA decay in E. coli. The enzyme interacts with the exonuclease polynucleotide phosphorylase (PNPase), enolase and RNA helicase B (RhlB) to form the degradosome. However, in Bacillus subtilis, this enzyme is absent, but it has other main endonucleases such as RNase J1 and RNase III. RNase III cleaves double-stranded RNA and family members are involved in RNA interference in eukaryotes. RNase II family members are ubiquitous exonucleases, and in eukaryotes, they can act as the catalytic subunit of the exosome. RNases act in different pathways to execute the maturation of rRNAs and tRNAs, and intervene in the decay of many different mRNAs and small noncoding RNAs. In general, RNases act as a global regulatory network extremely important for the regulation of RNA levels.

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