Mesoporous Silica Nanoparticles Improve Magnetic Labeling Efficiency in Human Stem Cells

Abstract

Tumblerlike magnetic/fluorescein isothiocyanate (FITC)-labeled mesoporous silica nanoparticles, Mag-Dye@MSNs, have been developed, which are composed of silica-coated core-shell superparamagnetic iron oxide (SPIO@SiO(2)) nanoparticles co-condensed with FITC-incorporated mesoporous silica. Mag-Dye@MSNs can label human mesenchymal stem cells (hMSCs) through endocytosis efficiently for magnetic resonance imaging (MRI) in vitro and in vivo, as manifested by using a clinical 1.5-T MRI system with requirements of simultaneous low incubation dosage of iron, low detection cell numbers, and short incubation time. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes, which can still be readily detected by MRI. Moreover, a higher MRI signal intensity decrease is observed in Mag-Dye@MSN-treated cells than in SPIO@SiO(2)-treated cells. This is the first report that MCM-41-type MSNs are advantageous to cellular uptake, as manifested by a higher labeling efficiency of Mag-Dye@MSNs than SPIO@SiO(2).

Effect of functionalised fluorescence-labelled nanoparticles on mesenchymal stem cell differentiation

“…However, cell differentiation was investigated only qualitatively or restricted to certain lineages. Fluorescence-labelled biocompatible mesoporous silica nanoparticles were reported to have no effect on osteogenic and adipogenic MSC differentiation [21,31]. In contrast, some authors found a negative influence on osteogenic differentiation after treatment with biphasic calcium phosphate particles, related to particle release from bioceramics [32].…”

“…Some authors reported an undisturbed differentiation capacity of MSC towards the osteogenic, adipogenic or chondrogenic lineage when treated with various particles [17,21,22]. In most of these studies, the investigation of the differentiation potential was limited, being only qualitative or restricted to only a small number of cellular differentiation markers.…”

Biodegradable magnetic-fluorescent magnetite/poly(dl-lactic acid-co-α,β-malic acid) composite nanoparticles for stem cell labeling

“…Several types of magnetic-fluorescent nanocomposites have been developed [42][43][44][45][46][47]. Lu et al have prepared a silica encapsulated commercial ferrofluid with the thickness of the silica shell controlled to between 2 and 100 nm by changing the concentration of the TEOS precursor [16].…”

Inhibitory Effects of Silica Nanoparticles Loaded with Hematoporphyrin on Breast Cancer Cell Line

“…The antioxidant effect of MSN is related to their biocompatibility (22,30). In previous studies, it has been shown that MSN possesses biocompatibility features (21,31). These results show that MSN without affecting healthy tissues circulates in the bloodstream and when reaches to the tumor cells, exerts its inhibitory effects.…”