Mesoflavibacter aestuarii sp. nov., a zeaxanthin-producing marine bacterium isolated from seawater

Lee, Ji Hee; Hwang, Yeoung Min; Baik, Keun Sik; Choi, Kap Seong; Ka, Jong-Ok; Seong, Chi Nam

doi:10.1099/ijs.0.061085-0

Cited by 20 publications

(14 citation statements)

References 21 publications

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“…Mesoflavibacter (Asker et al, 2007) appeared as paraphyletic in GBDP tree (Figure 8) because the type species of the genus, M. zeaxanthinifaciens (Asker et al, 2007), along with M . sabulilitoris (Park et al, 2014) were more closely related to Olleya (Nichols et al, 2005) than to M. aestuarii (Lee J. H. et al, 2014). When M. aestuarii and M .…”

Section: Resultsmentioning

confidence: 99%

Analysis of 1,000 Type-Strain Genomes Improves Taxonomic Classification of Bacteroidetes

et al. 2019

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Although considerable progress has been made in recent years regarding the classification of bacteria assigned to the phylum Bacteroidetes, there remains a need to further clarify taxonomic relationships within a diverse assemblage that includes organisms of clinical, piscicultural, and ecological importance. Bacteroidetes classification has proved to be difficult, not least when taxonomic decisions rested heavily on interpretation of poorly resolved 16S rRNA gene trees and a limited number of phenotypic features. Here, draft genome sequences of a greatly enlarged collection of genomes of more than 1,000 Bacteroidetes and outgroup type strains were used to infer phylogenetic trees from genome-scale data using the principles drawn from phylogenetic systematics. The majority of taxa were found to be monophyletic but several orders, families and genera, including taxa proposed long ago such as Bacteroides, Cytophaga, and Flavobacterium but also quite recent taxa, as well as a few species were shown to be in need of revision. According proposals are made for the recognition of new orders, families and genera, as well as the transfer of a variety of species to other genera. In addition, emended descriptions are given for many species mainly involving information on DNA G+C content and (approximate) genome size, both of which can be considered valuable taxonomic markers. We detected many incongruities when comparing the results of the present study with existing classifications, which appear to be caused by insufficiently resolved 16S rRNA gene trees or incomplete taxon sampling. The few significant incongruities found between 16S rRNA gene and whole genome trees underline the pitfalls inherent in phylogenies based upon single gene sequences and the impediment in using ordinary bootstrapping in phylogenomic studies, particularly when combined with too narrow gene selections. While a significant degree of phylogenetic conservation was detected in all phenotypic characters investigated, the overall fit to the tree varied considerably, which is one of the probable causes of misclassifications in the past, much like the use of plesiomorphic character states as diagnostic features.

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Section: Resultsmentioning

confidence: 99%

Analysis of 1,000 Type-Strain Genomes Improves Taxonomic Classification of Bacteroidetes

et al. 2019

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“…Mameliella play important roles in the degradation of aromatic compounds 41,42 and can accumulate beneficial poly-β-hydroxybutyrate (PHB) granules in cells 43 . Mesoflavibacter can produce zeaxanthin that have strong antioxidant and anticancer properties 44,45 . Interestingly, based on the KEGG pathway analysis, “nitrotoluene degradation” and “methane metabolism” were increased in the three SA groups, which indicated that dietary RS contribute to the enrichment of the beneficial bacteria that are involved in degrading toxins and producing beneficial metabolites.…”

Section: Discussionmentioning

confidence: 99%

Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch

Duan

Wang

Liu

et al. 2019

Sci Rep

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Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals. However, the roles of RS on shrimp intestine health is unknown. In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei . The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days. The results showed that dietary RS improved the morphology of the intestine mucosa. RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes ( proPO , ALF , Lys , HSP70 , Trx , Muc-1 , Muc-2 , Muc-5AC , Muc-5B , and Muc-19 ). A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial. Specifically, RS increased the abundances of Proteobacteria and decreased the abundance of Bacteroidetes. At the genus level, the beneficial bacteria ( Lutimonas , Ruegeria , Shimia , Mesoflavibacter , and Mameliella ) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens ( Formosa and Pseudoalteromonas ) were decreased in response to dietary RS. Our results revealed that dietary RS could improve the intestine health of L . vannamei , probably via modulating the intestine microbial composition and SCFAs contents, and enhancing the digestion and immunity of the shrimp.

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“…Subsequently, one further species of the genus Mesoflavibacter, Mesoflavibacter aestuarii, was described from seawater collected from the South Sea of South Korea (Lee et al, 2014). Phylogenetic analyses based on 16S rRNA gene sequences showed that the genus Mesoflavibacter belongs to the family Flavobacteriaceae of the phylum Bacteroidetes (Asker et al, 2007;Lee et al, 2014).…”

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“…that strain GJMS-9 T is a member of the genus Mesoflavibacter (Table 2, Fig. S1; Asker et al, 2007;Lee et al, 2014).…”

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Mesoflavibacter sabulilitoris sp. nov., isolated from seashore sand

Park

Jung

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, gliding and rod-shaped bacterial strain, designated GJMS-9 T , was isolated from seashore sand collected at Geoje island in the South Sea, South Korea. Strain GJMS-9 T grew optimally at 30 6C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJMS-9 T clustered with the type strain of Mesoflavibacter zeaxanthinifaciens, showing the highest sequence similarity of 99.1 %. Strain GJMS-9 T exhibited 16S rRNA gene sequence similarity of 96.5 % to the type strain of Mesoflavibacter aestuarii and of less than 96.1 % to the type strains of other recognized species. Strain GJMS-9 T contained MK-6 as the only menaquinone and iso-C 15 : 1 G, iso-C 16 : 0 3-OH, iso-C 15 : 0 and iso-C 17 : 0 3-OH as the major fatty acids. The polar lipid profile of strain GJMS-9 T containing phosphatidylethanolamine, one unidentified lipid and one unidentified glycolipid as major components was similar to that of the type strain of M. zeaxanthinifaciens. The DNA G+C content of strain GJMS-9 T was 32.2 mol% and its DNA-DNA relatedness with M. zeaxanthinifaciens DSM 18436 T was 38±6.1 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJMS-9 T is separated from other species of the genus Mesoflavibacter. On the basis of the data presented, strain GJMS-9 T is considered to represent a novel species of the genus Mesoflavibacter, for which the name Mesoflavibacter sabulilitoris sp. nov. is proposed. The type strain is GJMS-9 T (5KCTC 42117 T 5CECT 8597 T ).The genus Mesoflavibacter was proposed by Asker et al. (2007Asker et al. ( , 2008 with the description of a single species, Mesoflavibacter zeaxanthinifaciens, which was isolated from seawater collected on the Pacific coastline of Japan. Subsequently, one further species of the genus Mesoflavibacter, Mesoflavibacter aestuarii, was described from seawater collected from the South Sea of South Korea (Lee et al., 2014). Phylogenetic analyses based on 16S rRNA gene sequences showed that the genus Mesoflavibacter belongs to the family Flavobacteriaceae of the phylum Bacteroidetes (Asker et al., 2007;Lee et al., 2014). In this report, we describe a bacterial strain, designated GJMS-9 T , which was isolated from seashore sand collected at Geoje island in the South Sea, South Korea. Comparative 16S rRNA gene sequence analysis indicated that strain GJMS-9 T is phylogenetically most closely related to members of the genus Mesoflavibacter. The aim of the present work was to investigate the possibility that strain GJMS-9 T represents a novel species of the genus Mesoflavibacter by using a polyphasic characterization that included the determination of chemotaxonomic and other phenotypic properties, a detailed phylogenetic investigation based on 16S rRNA gene sequences, and DNA-DNA hybridization.Seashore sand was collected from Geoje island in the South Sea, South Korea, and used as a source f...

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Mesoflavibacter aestuarii sp. nov., a zeaxanthin-producing marine bacterium isolated from seawater

Cited by 20 publications

References 21 publications

Analysis of 1,000 Type-Strain Genomes Improves Taxonomic Classification of Bacteroidetes

Analysis of 1,000 Type-Strain Genomes Improves Taxonomic Classification of Bacteroidetes

Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch

Mesoflavibacter sabulilitoris sp. nov., isolated from seashore sand

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