Mesenteric adenitis caused by Yersinia pseudotubercolosis in a patient subsequently diagnosed with Crohn’s disease of the terminal ileum

Zippi, Maddalena; Colaiacomo, Maria Chiara; Marcheggiano, Adriana; Pica, Roberta; Paoluzi, P.; Iaiani, Giancarlo; Caprilli, R.; Maccioni, Francesca

doi:10.3748/wjg.v12.i24.3933

Cited by 16 publications

(14 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Infection by enteropathogenic Yersinia has been observed, on the other hand, in some patients with inflammatory bowel diseases 38 – 40. Higher rates of human pseudotuberculosis were also reported in geographical areas with a high incidence of these chronic immunopathologies 41 42.…”

Section: Discussionmentioning

confidence: 99%

Toll-like receptor 2 is critical for induction of Reg3 expression and intestinal clearance of Yersinia pseudotuberculosis

Dessein

Gironella

Vignal

et al. 2009

Gut

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Toll-like receptor 2 is critical for induction of Reg3 expression and intestinal clearance of Yersinia pseudotuberculosis

Dessein

Gironella

Vignal

et al. 2009

Gut

View full text Add to dashboard Cite

show abstract

“…Another consideration in this differential diagnosis of right lower quadrant abdominal pain accompanied by diarrhea and fever is acute ileitis caused by Yersinia infection. Yersinia infection may present with a clinical picture resembling appendicitis or the initial onset of appendicitis and there have been reports of Yersinia infections evolving into Crohn's disease [318]. At laparotomy, the acute Yersinia ileitis may be indistinguishable from Crohn's ileitis.…”

Section: Differential Diagnosismentioning

confidence: 99%

Inflammatory Bowel Disease

Stenson

Hanauer

Cohen

2008

Textbook of Gastroenterology

View full text Add to dashboard Cite

In the US, H. pylori vacA shows allelic variation in the signal sequence (which may be type sla, slb or s2) and the mid-region (type ml or m2). Previous PCR-based vacA mid-region typing classified most, but not all, Asian and South American strains tested as ml or m2. We now sought to investigate vacA mid-region diversity further.MotlmodL We studied 13 Japanese, 6 Chinese, 9 Thai, and 8 Peruvian H. pylori isolates. cagA was identified by colony hybridisation (CH), vacA signal sequence was typed by PCR, and vacA mid-region was typed proximally by CH and distally by PCR. Sections of the vacA mid-region from 8 strains were PCR-amplified, sequenced, and compared with known sequences from 8 other strains.Rcuts Of the 36 Asian and South American strains studied, 35 were cagA+ (the cagA-was Peruvian) and 35 were vacA sla (1 cagA + Peruvian was slb). vacA mid-regions from the 13 Japanese strains were not PCR-amplified by ml or m2-specific primers, but hybridised weakly with an ml probe. Sequence analysis of vacA from 1 Japanese strain revealed 91% nucleotide identity with the ml probe but only 71% identity with the m2 probe. The previously equivocal Thai and Peruvian strains also had ml-like mid-region sequences. A Chinese strain was ml in the proximal mid-region and m2 distally, showing a clear crossover site. Final mid-region types were: Japanese, all 13 ml; Chinese, 1 ml, 1 ml/m2, 4 m2; Thai, 3 ml, 6 m2; Peruvian 4 ml, 4 m2. Distal mid-region sequences of 16 strains, compared over 294 bp, clustered into 2 groups, ml and m2. Nucleotide identity between ml and m2 strains ranged from 73-78%. Within groups, m2 strains were less diverse than ml strains (m2 range 94-99.7%, ml 88-99.3%, p<0.001). Sequence analysis of 7 ml and 3 m2 strains over 1.1kb proximally showed maintenance of clustering outside the 294bp region C duions These Asian and South American strains are similar in terms of cagA status and vacA sla genotype, but fall into 2 vacA midregion groups. ml sequences are more diverse than m2, and thus may be phylogenetically older. The vacA sequence of 1 Chinese strain suggests recombination in vivo between ml and m2 alleles. Introduction: H.pylori antimicrobial susceptibility is an important determinant ofthe efficacy of eradication therapies'. The prevalence of antimicrobial resistance varies within the UK and may increase given the increased use of eradication therapy. This multicentre study assesses the prevalence and possible associations ofH.pylon antimicrobial resistance. Methods: H.pylon was isolated from antral biopsies of patients undergoing routine endoscopy and cultured according to standard microbiological methods. Antimicrobial resistance was determined using "E-tests" or disc tests (tinidazole only) with breakpoints defined by previous studies. Results: H.pyloni was isolated from 32% (1222/3823) of patients and antimicrobial susceptibility determined in 90% (1077/1222) Previous case-control studies of IBD have used hospital or community derived controls. There are obvious inherent biases in both metho...

show abstract

“…Two independent groups have shown increased frequency of DNA of Y. enterocolitica and Y. pseudotuberculosis in intestinal samples of CD patients, 22,23 and several case reports have linked Yersinia-induced ileitis and lymphadenitis to the early phases of clinical CD. 24,25 Yersinia pseudotuberculosis expresses two different virulenceassociated adhesion proteins anchored to the outer membrane, ie, invasin and YadA. Together, these two proteins mediate initial adhesion, uptake and translocation of Yersinia across the intestinal epithelium.…”

mentioning

confidence: 99%

Yersinia pseudotuberculosis induces transcytosis of nanoparticles across human intestinal villus epithelium via invasin-dependent macropinocytosis

Ragnarsson

Schoultz

Gullberg

et al. 2008

Laboratory Investigation

View full text Add to dashboard Cite

Crohn's disease is characterized by a defect in intestinal barrier function, where bacteria are considered the most important inflammation-driving factor. Enteric bacteria, including E. coli and Yersinia spp, affect tight junctions in enterocytes, but little is known about bacterial effects on the transcellular pathway. Our objective was to study the short-term effects of Y. pseudotuberculosis on uptake of nanoparticles across human villus epithelium. Monolayers of human colon epitheliumderived Caco-2 cells and biopsies of normal human ileum were studied after 2 h exposure to Y. pseudotuberculosis expressing (inv þ ) or lacking (invÀ) the bacterial adhesion molecule, invasin. Transepithelial transport of fluorescent nanoparticles (markers of transcytosis) was quantified by flow cytometry, and mechanisms explored by using inhibitors of endocytosis. Epithelial expressions of b1-integrin and particle uptake pathways were studied by confocal microscopy. The paracellular pathway was assessed by electrical resistance (TER), mannitol flux, and expression of tight junction proteins occludin and caludin-4 by confocal microscopy. Inv þ Y. pseudotuberculosis adhered to the apical surface of epithelial cells and induced transcytosis of exogenous nanoparticles across Caco-2 monolayers (30-fold increase, Po0.01) and ileal mucosa (268 ± 47% of control; Po0.01), whereas invÀ bacteria had no effect on transcytosis. The transcytosis was concentration-, particle size-and temperature-dependent, and possibly mediated via macropinocytosis. Y. pseudotuberculosis also induced apical expression of b1-integrin on epithelial cells. A slight drop in TER was seen after exposure to inv þ Y. pseudotuberculosis, whereas mannitol flux and tight junction protein expression was unchanged. In summary, Y. pseudotuberculosis induced apical expression of b1-integrin and stimulated uptake of nanoparticles via invasindependent transcytosis in human intestinal epithelium. Our findings suggest that bacterial factors may initiate transcytosis of luminal exogenous particles across human ileal mucosa, thus presenting a novel mechanism of intestinal barrier dysfunction.

show abstract

Mesenteric adenitis caused by Yersinia pseudotubercolosis in a patient subsequently diagnosed with Crohn’s disease of the terminal ileum

Cited by 16 publications

References 10 publications

Toll-like receptor 2 is critical for induction of Reg3 expression and intestinal clearance of Yersinia pseudotuberculosis

Toll-like receptor 2 is critical for induction of Reg3 expression and intestinal clearance of Yersinia pseudotuberculosis

Inflammatory Bowel Disease

Yersinia pseudotuberculosis induces transcytosis of nanoparticles across human intestinal villus epithelium via invasin-dependent macropinocytosis

Contact Info

Product

Resources

About