Mesenchymal to embryonic incomplete transition of human cells by chimeric OCT4/3 (POU5F1) with physiological co-activator EWS

Makino, Hidenari; Toyoda, Masashi; Matsumoto, Kenji; Saito, Hirohisa; Nishino, Koichiro; Fukawatase, Yoshihiro; Machida, Masakazu; Akutsu, Hidenori; Uyama, Taro; Miyagawa, Yoshitaka; Okita, Hajime; Kiyokawa, Nobutaka; Fukushima, Takashi; Ishikawa, Yuichi; Nakamura, Takuro; Umezawa, Akihiro

doi:10.1016/j.yexcr.2009.06.016

Cited by 52 publications

(56 citation statements)

References 49 publications

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“…PAE cells were cultured in EGM-2MV BulletKit (Lonza) containing 5% FBS. Human iPSCs from UtE, PAE, and AM cells were generated according to the procedures described by Yamanaka and colleagues (1) with slight modification (27,28,33). The iPSCs derived from MRC5, UtE, PAE, and AM cells were maintained in iPSellon medium (Cardio Inc.) supplemented with 10 ng/ml recombinant human basic fibroblast growth factor (Wako Pure Chemical Industries, Ltd.) on irradiated MEF feeder cells.…”

Section: Methodsmentioning

confidence: 99%

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Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

Tateno

Toyota

Saito

et al. 2011

Journal of Biological Chemistry

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192

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Induced pluripotent stem cells (iPSCs) can now be produced from various somatic cell (SC) lines by ectopic expression of the four transcription factors. Although the procedure has been demonstrated to induce global change in gene and microRNA expressions and even epigenetic modification, it remains largely unknown how this transcription factor-induced reprogramming affects the total glycan repertoire expressed on the cells. Here we performed a comprehensive glycan analysis using 114 types of human iPSCs generated from five different SCs and compared their glycomes with those of human embryonic stem cells (ESCs; nine cell types) using a high density lectin microarray. In unsupervised cluster analysis of the results obtained by lectin microarray, both undifferentiated iPSCs and ESCs were clustered as one large group. However, they were clearly separated from the group of differentiated SCs, whereas all of the four SCs had apparently distinct glycome profiles from one another, demonstrating that SCs with originally distinct glycan profiles have acquired those similar to ESCs upon induction of pluripotency. Thirty-eight lectins discriminating between SCs and iPSCs/ESCs were statistically selected, and characteristic features of the pluripotent state were then obtained at the level of the cellular glycome. The expression profiles of relevant glycosyltransferase genes agreed well with the results obtained by lectin microarray. Among the 38 lectins, rBC2LCN was found to detect only undifferentiated iPSCs/ESCs and not differentiated SCs. Hence, the high density lectin microarray has proved to be valid for not only comprehensive analysis of glycans but also diagnosis of stem cells under the concept of the cellular glycome.

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Section: Methodsmentioning

confidence: 99%

“…Immunocytochemistry-Immunocytochemical analysis was performed as described previously (29,33,38). Human iPSCs were fixed with 4% paraformaldehyde in PBS for 10 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

Tateno

Toyota

Saito

et al. 2011

Journal of Biological Chemistry

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192

224

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“…Cell Culture R1 mESC lines [38] were maintained on mouse embryonic fibroblasts (MEFs) inactivated with 10 lg/ml mitomycin C (Sigma, Sigma-Aldrich, St. Louis, MO [39] were maintained on inactivated MEFs in hiPSC medium (DMEM/F12 supplemented with 20% Knockout Serum Replacement [Invitrogen], 2 mM L-glutamine [Invitrogen], 1% penicillin/streptomycin, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids) with 10 ng/ml FGF2 (Wako, Osaka, Japan, www.wako-chem.co.jp).…”

Section: Methodsmentioning

confidence: 99%

LacdiNAc (GalNAcβ1-4GlcNAc) Contributes to Self-Renewal of Mouse Embryonic Stem Cells by Regulating Leukemia Inhibitory Factor/STAT3 Signaling

et al. 2011

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Self-renewal of mouse embryonic stem cells (mESCs) is maintained by leukemia inhibitory factor (LIF)/signal transducer and activator of transcription (STAT3) signaling. However, this signaling control does not function in neither mouse epiblast stem cells (mEpiSCs) nor human ESCs (hESCs) or human induced pluripotent stem cells (hiPSCs). To date, the underlying molecular mechanisms that determine this differential LIF-responsiveness have not been clarified. Here, we show that the cell surface glycan LacdiNAc (GalNAcb1-4GlcNAc) is required for LIF/ STAT3 signaling. Undifferentiated state mESCs expressed LacdiNAc at a higher level than differentiated state cells. Knockdown of b4GalNAc-T3 reduced LacdiNAc expression and caused a decrease in LIF/STAT3 signaling that lessened the rate of self-renewal of mESCs. A biochemical analysis showed that LacdiNAc expression on LIF receptor (LIFR) and gp130 was required for the stable localization of the receptors with lipid raft/caveolar components, such as caveolin-1. This localization is required for transduction of a sufficiently strong LIF/STAT3 signal. In primed state pluripotent stem cells, such as hiPSCs and mEpiSC-like cells produced from mESCs, LacdiNAc expression on LIFR and gp130 was extremely weak and the level of localization of these receptors on rafts/caveolae was also low. Furthermore, knockdown of b4GalNAc-T3 decreased LacdiNAc expression and reduced the efficiency of reversion of primed state mEpiSC-like cells into naïve state mESCs. These findings show that the different LIF-responsiveness of naïve state (mESCs) and primed state (mEpiSCs, hESCs, and hiPSCs) cells is dependent on the expression of LacdiNAc on LIFR and gp130 and that this expression is required for the induction and maintenance of the naïve state. STEM CELLS 2011;29:641-650 Disclosure of potential conflicts of interest is found at the end of this article.

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“…Recently, we generated two hybridomas using a hiPS cell line (Tic) (10,11) as an antigen. These hybridomas produced antibodies that specifically bound to hiPS/ES cells but exhibited little or no binding to hEC cells (12).…”

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confidence: 99%

A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells

Matsumoto

Nakao

Kawabe

et al. 2015

Journal of Biological Chemistry

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Background: Carbohydrate epitopes are often used as markers for characterization of hiPS cells. Results: A mouse IgG1 antibody (R-17F) was raised using hiPS cells as an antigen. Conclusion: R-17F recognizes lacto-N-fucopentaose I on glycolipid and exhibits a cytotoxic effect on hiPS/ES cells. Significance: R-17F may be beneficial for safer regenerative medicine by eliminating residual undifferentiated hiPS/ES cells, which are a risk factor for carcinogenesis.

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Mesenchymal to embryonic incomplete transition of human cells by chimeric OCT4/3 (POU5F1) with physiological co-activator EWS

Cited by 52 publications

References 49 publications

Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

LacdiNAc (GalNAcβ1-4GlcNAc) Contributes to Self-Renewal of Mouse Embryonic Stem Cells by Regulating Leukemia Inhibitory Factor/STAT3 Signaling

A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells

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